ABSTRACT
Background: Shiga toxin producing Escherichia coli O157:H7 (STEC) is considered the most prevalent food borne pathogen that has gained increasing attention worldwide in recent years. Methods: A cross-sectional study was carried out at Bedele Municipal abattoir on cattle that were reported healthy from detailed ante-mortem inspections and having various body conditions scores. A total of 516 samples were collected and examined after enriched in modified peptone water. Following an enrichment, the samples were plated onto MacConkey agar and then onto Eosin methylene blue agar. Finally after a few similar procedures, 14 E. coli O157:H7 (STEC) isolates were confirmed through latex agglutination test. The collected data were analyzed using SPSS version 20 statistical software. Results: This study finding revealed that the overall prevalence of E. coli O157:H7 out of 516 samples was found to be 2.7%. However, on sample type basis, the prevalence of E. coli O157:H7 from feacal samples, carcass swabs, butcher hand swabs and knife swabs were 4.7%, 3.3%, 1.1% and 1.1%, respectively. It was also found that that the prevalence of E. coli O157:H7 was significantly affected by age groups of slaughtered cattle (p<0.05). Moreover, in vitro antimicrobial susceptibility test result on average showed that almost all of E. coli O157:H7 isolates were highly susceptible to kanamycin and no resistance was shown to ciprofloxacin and gentamicin. Finally, the conventional PCR detection of stx1, st2 and hylA genes revealed that only 21.4% and 14.3% were found to contain stx1 and hylA genes respectively. Conclusion: To wrap up, this study showed that Shiga toxin producing E. coli O157:H7 (STEC) isolates were found with almost low overall prevalence rate from all sample sources in this study site. Therefore, improving abattoir facilities and slaughter house workers' personal hygiene are recommended to curtail E. coli O157:H7 meat contamination in this abattoir.
ABSTRACT
This study was conducted to examine the effect of feeding a mixture of different proportions of Ficus sur fruits (FSF) and ground maize grain (MG) on intake, digestibility, growth, and blood profile on Yorkshire pigs. Dietary treatments comprised 100 % FSF and 0 % MG (100FSF), 67 % FSF and 33 % MG (67FSF), 33 % FSF and 67 % MG (33FSF), and 0 % FSF and 100 % MG (0FSF). Noug cake and soybean meal were included to meet nutrient requirement of the animals at isonitrogenous (18 % crude protein (CP)) level with graded levels of energy. Twenty pigs with an average initial weight of 27.75 ± 1.4 kg were grouped according to their body weight, and animals from each group were randomly assigned to four dietary treatments. There was no difference (P > 0.05) in dry matter, crude protein, and metabolizable energy intakes among the treatments. Organic matter (P < 0.05), ether extract (P < 0.01), and nitrogen-free extract (NFE) intakes were higher (P < 0.0001) for pigs fed with 0FSF than 100FSF. The digestibility of dry matter, crude protein, ether extract, and NFE did not differ (P > 0.05) among treatments. However, organic matter digestibility (P < 0.05) was highest for pigs in 0FSF than 100FSF. Conversely, crude fiber digestibility was in the reverse trend. No significant differences were observed in body weight gain among treatments (P > 0.05). The blood counts and sera metabolites of pigs across the treatments fall within the optimum range. Packed cell volume (PCV) and hemoglobin (Hgb) were higher, but creatinine and cholesterol concentrations were lower in 100FSF than 0FSF indicating better health conditions in pigs fed with FSF. It is, therefore, concluded that feeding FSF has comparable effects with MG on the metabolic performance, growth, and blood profile of pigs.
