ABSTRACT
La hipertensión pulmonar asociada es un padecimiento poco frecuente en los pacientes con lupus (LES), que por su morbimortalidad conlleva un pronóstico grave en el embarazo y posparto inmediato. El aumento de la resistencia arterial pulmonar asociado a los cambios fisiológicos cardiovasculares del fin del embarazo y del trabajo de parto generan una situación clínica de dificultoso manejo...Nuestro objetivo es presentar el caso de una paciente con LES previamente diagnosticado, en la que se desarrolla hipertensión pulmonar asociada (HPA)al final del segundo trimestre de un embarazo gemelar, que responde adecuadamente a sildenafil y evoluciona favorablemente (ella y sus dos niños) luego de realizarse cesárea programada en la semana 32.(AU)
Subject(s)
Adult , Humans , Female , Hypertension, Pulmonary/diagnosis , Hypertension, Pulmonary/pathology , Lupus Erythematosus, Systemic/diagnosis , Pregnancy Complications/pathologyABSTRACT
La hipertensión pulmonar asociada es un padecimiento poco frecuente en los pacientes con lupus (LES), que por su morbimortalidad conlleva un pronóstico grave en el embarazo y posparto inmediato. El aumento de la resistencia arterial pulmonar asociado a los cambios fisiológicos cardiovasculares del fin del embarazo y del trabajo de parto generan una situación clínica de dificultoso manejo...Nuestro objetivo es presentar el caso de una paciente con LES previamente diagnosticado, en la que se desarrolla hipertensión pulmonar asociada (HPA)al final del segundo trimestre de un embarazo gemelar, que responde adecuadamente a sildenafil y evoluciona favorablemente (ella y sus dos niños) luego de realizarse cesárea programada en la semana 32.
Subject(s)
Adult , Humans , Female , Hypertension, Pulmonary/diagnosis , Hypertension, Pulmonary/pathology , Lupus Erythematosus, Systemic/diagnosis , Pregnancy ComplicationsABSTRACT
Multiple endocrine neoplasia type 1 (MEN1) is an inherited syndrome characterized by development of multiple endocrine tumors in affected individuals. The gene responsible for the disease has been mapped to chromosome 11q13 by linkage analysis, but the gene itself has not yet been identified. We allelotyped 33 affected individuals from an extensive MEN1 kindred using eight polymorphic markers located on chromosome 11q13, including two new markers (D11S4907 and D11S4908) that we derived and mapped to the SEA-D11S913 region. Analysis of affected individuals revealed two separate recombination events, providing new centromeric and telomeric boundaries for the MEN1 gene. The present data indicate the MEN1 gene is located between markers D11S1883 and D11S4907, an approximate 2 Mb region on chromosome 11q13.
Subject(s)
Chromosomes, Human, Pair 11/genetics , Haplotypes/genetics , Multiple Endocrine Neoplasia Type 1/genetics , Alleles , Chromosome Mapping , DNA, Neoplasm/genetics , Female , Genetic Markers , Humans , Male , Pedigree , Polymorphism, Genetic , Recombination, GeneticABSTRACT
Levuglandin (LG) E2 is rapidly sequestered by covalent binding with proteins. The reaction of LGE2 with a protein in neutral aqueous solution exhibits two phases. A metastable adduct rapidly accumulates initially. In the second phase, a protein-bound pyrrole is generated. Pyrrole formation and stability were monitored with an immunoassay using antibodies that were raised against a stable isostere. That LG-derived pyrroles are the major products (> 76%) of the LGE2-protein reaction is suggested by the level of antibody binding found for LG-protein adducts compared with that found for a pyrrole derived from LGE2 and 6-amino-1-hexanol. Because the initial metastable LG-protein adduct is a reactive electrophile, it can be trapped with amines, such as glycine, to give stable ternary adducts that do not cross-react with the antibodies. Although highly alkylated pyrroles are chemically sensitive compounds, the protein-bound LG-derived pyrrole appears to be stable in aqueous solution at pH 7.4. Thus, it shows no decrease in immunoreactivity over several weeks. This discovery leads to the expectation that such pyrroles will accumulate in vivo, especially in proteins that do not turn over rapidly. Thus, the LG-derived protein-bound pyrrole may be a useful marker of oxidative lipid damage, and an immunoassay for this post-translational protein modification can be exploited as a mild, sensitive method for detecting and quantifying the generation of LGs in chronic inflammatory states.
Subject(s)
Prostaglandins E/metabolism , Pyrroles/metabolism , Animals , Drug Stability , Enzyme-Linked Immunosorbent Assay , Hemocyanins/immunology , Hemocyanins/metabolism , Prostaglandins E/immunology , Protein Binding , Pyrroles/chemistry , RabbitsABSTRACT
Infection with transmissible gastroenteritis virus (TGEV) was present in some pigs on arrival at a feeder pig farm and was well established three weeks later. TGE infection preceded Mycoplasma hyopneumoniae infection, which was not detected until three weeks after arrival. Severe lesions of enzootic pneumonia were observed at the end of the fattening period.A trial was subsequently done in six-week-old-pigs in order to evaluate the potentiating effect of TGEV infection on experimental M. hyopneumoniae infection. The effects of mycoplasmal infection were aggravated when associated with TGEV infection as determined by the extent of pneumonic lesions observed two weeks later.
ABSTRACT
A number of factors were studied in eight feeder pig herds, affected with severe or mild enzootic pneumonia, in order to identify those associated with this disease. Piggeries with poor facilities and management and where procurement of piglets was from sales barns were more severely affected with enzootic pneumonia than were those with good facilities and where pigs originated directly from breeding units. Serological tests and virus isolation revealed that all herds had been exposed to Mycoplasma hyopneumoniae and to many viruses; transmissible gastroenteritis virus infection was the only viral infection that was apparently associated with the severity of enzootic pneumonia and the performance observed in the herds.
ABSTRACT
Intracerebral and intraspinal inoculations of non-neuropathic and neuropathic strains of influenza virus into rhesus, patas and cercopithecus monkeys resulted in an acute focal ependymitis, choroiditis and meningitis followed by focal ependymal denuding without parenchymal involvement. Aqueductal stenosis and moderate hydrocephalus developed in two animals as sequelae of ependymal cell loss.
Subject(s)
Central Nervous System Diseases/pathology , Orthomyxoviridae , Animals , Brain/microbiology , Central Nervous System Diseases/etiology , Central Nervous System Diseases/microbiology , Cerebral Aqueduct/pathology , Choroid Plexus/pathology , Ependyma/pathology , Haplorhini , Hemagglutination Inhibition Tests , Injections , Injections, Intramuscular , Macaca mulatta , Meninges/pathology , Orthomyxoviridae/immunology , Orthomyxoviridae/isolation & purification , Spine , ThalamusABSTRACT
The election microscopic examination of allantoic fluid from embryonated hens' eggs inoculated with tissue homogenates from organs of birds suffering with infectious bronchitis, reveals the presence of coronaviruses as early as the first or the second passage. This method permits a rapid diagnosis and is as accurate as the standard technique.
Subject(s)
Bronchitis/veterinary , Poultry Diseases/diagnosis , RNA Viruses , Animals , Chick Embryo , Extraembryonic Membranes , Methods , Microscopy, Electron , Virus Diseases/veterinaryABSTRACT
Rhesus monkeys and ferrets were exposed to intranasal inoculation of several strains of egg-adapted avian, equine and human influenza viruses and to strains of mouse-adapted equine influenza viruses. Local replication of virus and seroconversion were observed in the majority of these animals. However, clinical infection was observed only in ferrets.
