[Effect of 1-D-ribofuranosyl-1,2,4-triazol-3-carboxamide on the synthesis of cellular macromolecules in cell culture]. / Vliianie 1-D-ribofuranozil-1,2,4-triazol-3-karboksamida na sintez kletochnykh makromolekul v kul'ture kletok.

Ribovirine (I-D-ribofuranosyl-1,2,4-triazol-3-carboxamide) possesses the distinct antiviral action against many strains of RNA- and DNA-containing viruses. Data on the effect of ribovirine on the synthesis of cellular macromolecules are presented. After treatment with ribovirine the synthesis of cellular RNA was inhibited by 80-85%. The synthesis of DNA and proteins was decreased less distinctly. The experiments were carried out using the primary tryptic-treated culture of fibroblasts from chicken embryos.

[Identification of RNAase-sensitive 20S RNA in a cell culture infected with Sindbis virus]. / Identifikatsiia chuvstvitel'noi k RNKaze 20S-RNK v kul'ture kletok, infitsirovannykh virusom sindbis

RNAse-sensitive 20S RNA component with molecular weight of 0.7-10(6) is found when analysing virus-specific RNAs isolated from cultured chicken embryo fibroblasts infected with Sindbis virus by means of gradient centrifugation and polyacrylamide gel electrophoresis.

[Effect of rimantadine on the synthesis of virus-specific RNA in the culture of cells infected with Sindbis virus]. / Vliianie rimantadina na sintez virusspetsificheskikh RNK v kul'ture kletok, infitsirovannykh virusom Sindbis

Effect of rimanthadine (alpha-methyl-I-adamantane methylamine) on the synthesis of virus specific RNA was studied in culture of cells, infected with Syndbis virus. Rimanthadine inhibits the synthesis of virus specific RNA beginning from the 3-rd hr of infection. A magnitude of inhibition from the 3-rd to the 7-th hrs of infection was practically the same and exceeded 50%. In the presence of rimanthadine "early" RNA was formed (with sedimentation constant of 20-14 S), where radioactivity was lowered as compared with control; formation of more "late" RNA peaks (43 S, 34 S and 26 S) was completely prevented within these periods of infection.

[Activity and properties of RNA-dependent RNA polymerase induced in cell cultures infected with Venezuelan equine encephalomyelitis virus]. / Izuchenie aktivnosti i nekotorykh svoistv RNK-zavisimoi RNK-polimerazy, indutirovannoi v kul'ture kletok, zarazhennykh virusom venesuél'skogo éntsefalomielita loshadei

The activity and properties of RNA-dependent RNA-polymerase in cells infected with Venezuelan equine encephalomyelitis virus were studied. The maximum activity of the enzyme was found 4-5 hours after infection. The effect of two synthetic drugs (rimantadine and diacetylaminocyclohexenon) with antiviral activity against some viruses on RNA-polymerase was studied. Rimantadine was shown to have no effect on the synthesis and activity of the enzyme. Diacetylaminocyclohexenon added into the growth medium was found to inhibit production of RNA-polymerase by 35-40% without reducing the activity of the enzyme in vitro.