ABSTRACT
UNLABELLED: Morphologically, polymorphic prostatic lipochrome pigment has been classified and subclassified in the last few years. Type 2B lipochrome pigment granules (LPGs) are frequently found in prostatic epithelium in patients who had died of AIDS. Intensive apoptosis is observed in the same epithelium which lends support to the hypothesis of heterophagocytic (apoptotic) origin of type 2B pigment granules. Detection of nuclear chromatin material is necessary for the differentiation of an autophagosomal from a heterophagosomal structure in the cellular cytoplasm. OBJECT OF THE STUDY: Application of in situ hybridization (ISH) for elucidating the origin of subtype 2B LPGs in the prostate epithelial cells of patients who had died of AIDS. METHODS: ISH was used on routine necropsic prostate epithelial samples from three patients who had died of AIDS. A DNA probe raised against total human DNA was employed. RESULTS: Multiple hybridization signals were detected in type 2B LPGs which shows the presence of nuclear material in those structures. The chromatin material localized to the periphery of pigment granules. CONCLUSION: Type 2B LPGs have a heterophagocytic origin and represent phagocytosed apoptotic bodies in the phase of phagolysosomal degradation. They can be used as a morphologic tissue marker of intensive epithelial apoptosis.
Subject(s)
Acquired Immunodeficiency Syndrome/physiopathology , Cytoplasmic Granules/metabolism , Lipofuscin/metabolism , Phagocytosis/physiology , Prostate/metabolism , Cytoplasmic Granules/ultrastructure , Epithelial Cells/metabolism , Epithelial Cells/ultrastructure , Histocytochemistry , Humans , In Situ Hybridization , Male , Prostate/pathology , Prostate/ultrastructureABSTRACT
The present paper describes a rare case of incipient perisinusoidal fibrosis of perihepatocellular type occurring in the Dubin-Johnson syndrome in a 63-year-old patient operated on six months previously for chronic calculous cholecystitis. The source of collagen formation was the activated sinusoidal Ito cells. Excluding chronic virus hepatitis and reactive cholecystic hepatitis, we believe that the fibrosis in this patient can be associated with the relatively old age of the patient and the duration of the disease.
Subject(s)
Jaundice, Chronic Idiopathic/complications , Liver Cirrhosis/complications , Collagen/physiology , Humans , Liver/pathology , Liver Cirrhosis/pathology , Liver Cirrhosis/physiopathology , Male , Middle AgedABSTRACT
The authors have described the first case of Kikuchi's disease in Bulgaria. The present study gives the clinicopathologic algorithm compiled by them to make the correct diagnosis and differentiate Kikuchi's disease from systemic lupus erythematosus lymphadenopathy.
Subject(s)
Algorithms , Histiocytic Necrotizing Lymphadenitis/pathology , Lupus Erythematosus, Systemic/pathology , Lymphatic Diseases/pathology , Biopsy, Needle , Bulgaria , Diagnosis, Differential , Histiocytic Necrotizing Lymphadenitis/diagnosis , Humans , Immunohistochemistry , Lupus Erythematosus, Systemic/diagnosis , Lymphatic Diseases/diagnosis , Severity of Illness IndexABSTRACT
We describe a combination of epithelial cell apoptosis and intracytoplasmic inclusions in prostatic epithelium in 6 patients who died from the acquired immunodeficiency syndrome. Two different types of apoptosis were detected: simple cell shrinkage and exploding glandular cells. No intracellular or extracellular viral particles were detected, either ultrastructurally or immunohistochemically. Intracytoplasmic inclusions are apoptotic bodies in a state of degradation and in close association with lipofuscin. The cell degeneration we observed confirms the theory that increased apoptotic cell depletion is responsible for weight loss in the acquired immunodeficiency syndrome. In the prostate itself, the combination of excessive apoptosis and active phagosomal digestion of apoptotic bodies presents a "human model" of postcastration rat ventral prostate, under the conditions of severe immune deficiency.
