ABSTRACT
O artigo não apresenta resumo.
ABSTRACT
Oral focal epithelial hyperplasia is a rare and seldom reported disease in animals and humans induced by a papillomavirus. The present report is the first description of this disease in a Neotropical primate, a howler monkey (Alouatta fusca). The diagnosis was based on gross and microscopic findings. The generic papillomavirus antigen was identified by immunohistochemistry and was found not to be related to any human papillomavirus DNA tested by in situ hybridization. This virus is probably a specific papillomavirus of the howler monkey (HMPV).
Subject(s)
Alouatta , Focal Epithelial Hyperplasia/veterinary , Monkey Diseases/pathology , Papillomaviridae/isolation & purification , Papillomavirus Infections/veterinary , Animals , Antigens, Viral/analysis , Brazil , Fatal Outcome , Focal Epithelial Hyperplasia/pathology , Focal Epithelial Hyperplasia/virology , In Situ Hybridization/veterinary , Male , Papillomaviridae/genetics , Papillomaviridae/immunology , Papillomavirus Infections/pathology , RabbitsABSTRACT
Computerized image analysis was performed on freeze-fractured nuclei isolated from normal rat liver and rat liver with diethylnitrosamine induced hepatocarcinomas. Chromatin particles belonging to heterochromatin and interchromatin nuclear regions were measured, distributed into histograms and statistically processed. The ratio between nucleosome filaments (5-10 nm range diameter) and solenoid fibres (25-35 nm range) and Tukey's fitting line were calculated. The N/S ratio shows an inverse trend in treated nuclear samples with respect to the controls indicating that the malignancy progression is accompanied by characteristic derangement of the chromatin arrangement which can be evaluated by morphometric analysis.
