The influence of climate changes on heavy metals accumulation in Alosa immaculata from the Danube River Basin.

This research report provides a comprehensive overview of the historical trends in heavy metal concentrations in the Pontic shad (Alosa immaculata) populations from both the Danube River and the Black Sea, while also exploring the potential influence of global warming on metal accumulation. Through bibliometric modeling analysis, it reveals significant limitations in existing international research, particularly the lack of comprehensive data on the impact of hydroclimatic changes on heavy metal accumulation in Alosa immaculata. Recognizing the critical importance of studies on heavy metal bioaccumulation in Danube shad, this research underscores their significance in defining tolerance thresholds, quantifying the impact of toxic elements along the aquatic food chain, and enhancing the economic sustainability of ichthyofauna monitoring efforts. Furthermore, these studies contribute invaluable insights into the complex dynamics of aquatic ecosystems, offering essential decision-making support for optimizing commercial fishing management practices on the Danube and ensuring robust support systems for industrial fishing endeavors.

The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in Cyprinus carpio L.

Berberine (BBR) and silymarin (SM) are natural compounds extracted from plants known for their antioxidant and chemoprotective effects on the liver. The present study aimed to investigate the beneficial properties of BBR and SM and the association of BBR with SM on liver function using fish as "in vivo" models. Moreover, the study investigated their hepatoprotective role after acetaminophen (APAP) exposure. For this purpose, the fish (N = 360; 118.4 ± 11.09 g) were fed with control or experimental diets for 9 weeks. In the experimental diets, the feed was supplemented with either SM (1 g/kg feed), BBR (100 and 200 mg/kg feed), or a combination of BBR with SM (SM 1 g/kg feed + BBR 100 mg/kg feed and, respectively, SM 1 g/kg feed + BBR 200 mg/kg feed). After the feeding trial, seven fish from each tank were randomly selected and exposed to a single APAP dose. The selected serum biochemical markers, oxidative stress markers, and lysozyme activity were used to evaluate the efficiency of the supplements on carp's health profile, particularly regarding the hepatopancreas function. Our results showed that the inclusion of SM and BBR (either as a single or in combination) reduced the serum contents of total cholesterol, triglyceride, and alanine transaminase. An increase in the high-density cholesterol was observed after the administration of BBR or BBR in association with SM. Both supplements showed hepatoprotective activity against APAP-induced hepatotoxicity, especially BBR. The ameliorative effects of SM (1 g) in association with BBR (100 mg) were highlighted by the modulation of the nonspecific immune system and oxidative stress alleviation after APAP exposure.