ABSTRACT
(-)-5-Methylmellein (1) and its new dimer (2) were isolated from cultures of the basidiomycete Inonotus sinensis. Their structures were elucidated on the basis of extensive spectroscopic methods including UV, IR, HR-EI-MS, 1D NMR and 2D NMR. The structure of Compound 2 was determined by single-crystal X-ray crystallographic analysis. Compound 2 was tested for the cytotoxicities against five human cancer cell lines.
Subject(s)
Basidiomycota , Inonotus , Humans , Molecular Structure , Basidiomycota/chemistry , Cell Line, TumorABSTRACT
Two new tremulane-type sesquiterpenes, irlactin L (1) and irlactin M (2) were isolated from cultures of the fungus Irpex lacteus, together with one known compound, 6-hydroxy-2,6-dimethyloct-7-enoic acid (3). Their structures were elucidated by spectroscopic data analysis. Compounds 1-2 were tested for their cytotoxicities against five human cancer cell lines and for their inhibitory activities against isozymes of 11ß-hydroxysteroid dehydrogenases (11ß-HSD).[Formula: see text].
Subject(s)
Polyporales , Sesquiterpenes , Fungi , Molecular Structure , Sesquiterpenes/pharmacologyABSTRACT
Two new compounds, daedatrin K (1) and 2-hydroxy-1-(5-(hydroxymethyl)furan-2-yl)propan-1-one (2), were isolated from cultures of the basidiomycetes Daedaleopsis tricolor. The new structures were elucidated on the basis of extensive spectroscopic methods. At the same time, two compounds were tested for their cytotoxicities against five human cancer cell lines. [Formula: see text].
Subject(s)
Basidiomycota , Humans , Molecular StructureABSTRACT
A new cyclohexenone, named phomaligol D (1), together with two known compounds, kojic acid (2) and phomaligol A (3) were isolated from the tin mine tailings-derived fungus Aspergillus flavus YIM DT 10012. Their structures were elucidated by detailed analysis of spectroscopic data.
Subject(s)
Aspergillus flavus/chemistry , Cyclohexenes/isolation & purification , Cyclohexenes/chemistry , Mining , Molecular Structure , Pyrones/isolation & purification , TinABSTRACT
A new tremulane sesquiterpenoid, named irlactam A (1), was isolated from cultures of the fungus Irpex lacteus. The new structure was elucidated by spectroscopic data analysis. The compound was tested for its cytotoxicities on HL-60, SMMC-7721, A-549, MCF-7, and SW480 cells and for its inhibitory activity against isozymes of 11 ß-hydroxysteroid dehydrogenases (11 ß-HSD).
Subject(s)
Fungi/chemistry , Sesquiterpenes/isolation & purification , 11-beta-Hydroxysteroid Dehydrogenases/antagonists & inhibitors , Cell Line, Tumor , Drug Screening Assays, Antitumor , Fungi/cytology , Humans , Isoenzymes/antagonists & inhibitors , Molecular Structure , Sesquiterpenes/chemistry , Sesquiterpenes/pharmacologyABSTRACT
A new pregnane steroid, named aspergillon A (1), together with two known compounds, (22E,24R)-ergosta-5,7,22-trien-3ß-ol (2) and (22E, 24R)-ergosta-4,6,8(14),22-tetraen-3-one (3) were isolated from cultures of the tin mine tailings-associated fungus Aspergillus versicolor. The new structure and absolute configuration were determined with the help of extensive spectroscopic analyses and quantum chemical calculations of the electronic circular dichroism (ECD) spectra.
Subject(s)
Aspergillus/chemistry , Pregnanes/isolation & purification , Steroids/isolation & purification , Circular Dichroism , Fungi , Mining , Molecular Structure , Pregnanes/chemistry , Spectrum Analysis , Steroids/chemistryABSTRACT
A new guaiane sesquiterpene (1) was isolated from cultures of the fungus Fulvifomes kanehirae. Its structure was elucidated by spectroscopic methods including extensive 2D-NMR techniques. Meanwhile, the compound was tested for its inhibitory activity against isozymes of 11ß-hydroxysteroid dehydrogenases (11ß-HSD).
Subject(s)
11-beta-Hydroxysteroid Dehydrogenases/antagonists & inhibitors , Basidiomycota/chemistry , Sesquiterpenes, Guaiane/chemistry , Basidiomycota/cytology , Culture Techniques , Isoenzymes , Magnetic Resonance Spectroscopy , Molecular Structure , Sesquiterpenes, Guaiane/isolation & purification , Sesquiterpenes, Guaiane/pharmacologyABSTRACT
Three new isopimarane diterpenoid lactones, named inonotolides A-C (1-3), were isolated from cultures of the basidiomycete Inonotus sinensis. Their structures were elucidated on the basis of extensive spectroscopic studies and the structure of inonotolide A (1) was confirmed by single-crystal X-ray crystallographic analysis. All compounds were evaluated for their cytotoxicities against five human cancer cell lines.
Subject(s)
Basidiomycota/chemistry , Diterpenes/isolation & purification , Lactones/isolation & purification , Cell Line, Tumor , Humans , Molecular StructureABSTRACT
Two new polyols, (2E,4E)-octa-2,4-diene-1,6,7-triol (1) and (E)-7,8-dihydroxyoct-5-en-2-one (2) were isolated from cultures of the basidiomycete Daedaleopsis tricolor. Their structures were elucidated by spectroscopic methods including extensive 2D NMR techniques. Two compounds showed no significant activity against five human cancer cell lines.
Subject(s)
Alcohols/chemistry , Alkenes/chemistry , Alkenes/pharmacology , Antineoplastic Agents/pharmacology , Coriolaceae/chemistry , Alcohols/pharmacology , Alkadienes/chemistry , Alkadienes/pharmacology , Antineoplastic Agents/chemistry , Cell Line, Tumor , Coriolaceae/cytology , Drug Screening Assays, Antitumor , Humans , Magnetic Resonance Spectroscopy , Molecular StructureABSTRACT
Three cyclic dipeptides (1-3), including one new compound (1) were isolated from cultures of the basidiomycetes Coprinus plicatilis. Their structures were elucidated by spectroscopic methods, including extensive 2D NMR techniques. At the same time, all compounds were tested for their cytotoxicities against five human cancer cell lines.
Subject(s)
Antineoplastic Agents/pharmacology , Coprinus/chemistry , Dipeptides/pharmacology , Peptides, Cyclic/pharmacology , Antineoplastic Agents/chemistry , Cell Line, Tumor , Dipeptides/chemistry , Dipeptides/isolation & purification , Drug Screening Assays, Antitumor , Humans , Magnetic Resonance Spectroscopy , Peptides, Cyclic/chemistry , Peptides, Cyclic/isolation & purificationABSTRACT
A new phenylspirodrimane dimer, named stachartarin A (1), was isolated from cultures of the tin mine tailings-associated fungus Stachybotrys chartarum. Its structures were elucidated by means of spectroscopic methods. At the same time, the compound was tested for its cytotoxicity against HL-60, SMMC-7721, A-549, MCF-7, and SW480 cells.
Subject(s)
Spiro Compounds/isolation & purification , Stachybotrys/chemistry , Cell Line, Tumor , Humans , Mining , Molecular Structure , Secondary Metabolism , TinABSTRACT
Five new tremulane sesquiterpenes, named irlactins F-J (1-5), were isolated from cultures of the basidiomycete Irpex lacteus together with two known analogues (6 and 7). Structures and relative configurations of compounds 1-5 were elucidated by spectroscopic data analysis. Compund 4 exhibited moderate cytotoxicities on HL-60, SMMC-7721, A-549, MCF-7, and SW480 cells with IC50 values of 16.23, 20.40, 25.55, 19.05, and 18.58µM, respectively.
Subject(s)
Polyporales/chemistry , Sesquiterpenes/chemistry , Cell Line, Tumor , Humans , Molecular Structure , Sesquiterpenes/isolation & purificationABSTRACT
A new cadinane sesquiterpenoid, named panutorulon A (1), was isolated from cultures of the basidiomycete Panus conchatus. The new structure was elucidated by means of spectroscopic methods. Compound 1 was tested for its cytotoxicity against five human cancer cell lines and for its inhibitory activity against isozymes of 11ß-hydroxysteroid dehydrogenases (11ß-HSD).
Subject(s)
Basidiomycota/chemistry , Sesquiterpenes/isolation & purification , 11-beta-Hydroxysteroid Dehydrogenases/antagonists & inhibitors , Antineoplastic Agents/isolation & purification , Antineoplastic Agents/pharmacology , Cell Line, Tumor , Humans , Isoenzymes/antagonists & inhibitors , Molecular Structure , Polycyclic Sesquiterpenes , Sesquiterpenes/chemistry , Spectrum AnalysisABSTRACT
Stachartone A (1), a novel phenylspirodrimane dimer, was isolated from cultures of the tin mine tailings-associated fungus Stachybotrys chartarum. Its structure was elucidated by means of spectroscopic methods. At the same time, compound (1) was tested for its cytotoxicity against five human cancer cell lines.
Subject(s)
Sesquiterpenes/isolation & purification , Stachybotrys/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/isolation & purification , Antineoplastic Agents/pharmacology , Cell Line , Cell Line, Tumor , Dimerization , Humans , Mining , Molecular Structure , Polycyclic Sesquiterpenes , Sesquiterpenes/chemistry , Spiro Compounds/chemistry , Spiro Compounds/isolation & purificationABSTRACT
Three new drimane sesquiterpenoids, 12-hydroxyalbrassitriol (1), drim-8(12)-en-6ß,7α, 9α,11-tetraol (2), and drim-68(12)-dien-9α,11-diol (3), along with one known analog albrassitriol (4), were isolated from cultures of the tin mine tailings-associated fungus Penicillium sp. The new structures were determined on the basis of extensive spectroscopic analyses. All compounds were tested for their cytotoxicities against five human cancer cell lines.
Subject(s)
Antineoplastic Agents/isolation & purification , Penicillium/chemistry , Sesquiterpenes/isolation & purification , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , China , Drug Screening Assays, Antitumor , HL-60 Cells , Humans , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Polycyclic Sesquiterpenes , Sesquiterpenes/chemistry , Sesquiterpenes/pharmacologyABSTRACT
Two new sesquiterpenoids, trefoliol B (1) and trefoliol C (2), together with known echinocidin A (3), were isolated from cultures of the basidiomycetes Tremella foliacea. The new structures were elucidated on the basis of extensive spectroscopic methods. At the same time, trefoliol B (1) and echinocidin A (3) were tested for their cytotoxicities against five human cancer cell lines and for their inhibitory activities against isozymes of 11ß-hydroxysteroid dehydrogenases (11ß-HSD). No compound showed significant activity (IC50 > 40 µM). Compound 1 showed moderate inhibitory activities against 11ß-HSD1 (human IC50 = 13.1 µM; mouse IC50 = 91.8 µM).
Subject(s)
11-beta-Hydroxysteroid Dehydrogenase Type 1/antagonists & inhibitors , Antineoplastic Agents/isolation & purification , Basidiomycota/chemistry , Sesquiterpenes/isolation & purification , Animals , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Drug Screening Assays, Antitumor , Female , Humans , Isoenzymes , Mice , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Sesquiterpenes/chemistry , Tetrazolium Salts/pharmacology , Thiazoles/pharmacologyABSTRACT
Objective: To study the chemical constituents from fruiting bodies of Postia balsamea. Methods: The fruiting bodies of Postia balsamea was extracted,the compounds were isolated by silica gel column chromatography. The structures were elucidated by the spectral analysis. Results: Seven compounds were isolated and identified as botulin ( 1),( 22 E,24R)-5α,8α-epidioxy-ergosta-6,22-dien-3ß-ol( 2),( 22 E,24R)-5α,8α-epidioxy-ergosta-6,9( 11),22-trien-3ß-ol( 3),( 22 E,24R)-ergosta-7,22-dien-3ß-ol( 4), betulinic acid( 5),( 22 E,24R)-ergosta-5,7,22-trien-3ß-ol ( 6) and stearic acid( 7). Conclusion: All the compounds are isolated from this species for the first time.
Subject(s)
Basidiomycota , Fruiting Bodies, Fungal , Pentacyclic Triterpenes , Triterpenes , Betulinic AcidABSTRACT
Four cadinane-type sesquiterpenes and four 13-carbon γ-lactones, together with three known compounds, were isolated from cultures of the basidiomycete Trichaptum pargamenum. Their structures were elucidated on the basis of extensive spectroscopic methods. The absolute configurations of two of the cadinene type sesquiterpenes 1 and 3 were confirmed by single crystal X-ray diffractions.
Subject(s)
Lactones/isolation & purification , Sesquiterpenes/isolation & purification , Basidiomycota/chemistry , Cell Line, Tumor , Cell Survival/drug effects , Crystallography, X-Ray , Fruiting Bodies, Fungal/chemistry , Humans , Lactones/chemistry , Lactones/pharmacology , Magnetic Resonance Spectroscopy , Molecular Structure , Polycyclic Sesquiterpenes , Sesquiterpenes/chemistry , Sesquiterpenes/pharmacologyABSTRACT
Three new compounds, vibranether (1), myrrhlalkyldiol (2), vibralactone H (3), together with three known compounds, 2-methyl-6-p-tolylheptane-2,3-diol (4), 2-hydroxy-2-methyl-6-p-tolylheptan-3-one (5), and vibralactone (6), were isolated from the cultures of the basidiomycete Boreostereum vibrans. Their structures were determined on the basis of spectroscopic evidences (1D and 2D NMR, HRMS, UV, and IR data), chemical methods, and the literature data. The new compounds displayed no significant cytotoxicities against five human cancer cell lines (IC50>40 µM).
Subject(s)
Basidiomycota/chemistry , Lactones/isolation & purification , Drug Screening Assays, Antitumor , Humans , Lactones/chemistry , Lactones/pharmacology , Molecular Structure , Nuclear Magnetic Resonance, BiomolecularABSTRACT
Four new drimane sesquiterpenoids, named as funatrols A-D, together with isodrimenediol, were isolated from cultures of the fungus Funalia trogii. The new structures were elucidated by means of spectroscopic methods. All compounds were tested for their cytotoxicities against five human cancer cell lines.
