ABSTRACT
Nanomodification and amorphization are vital for improving the hydrogenation properties of magnesium (Mg)-based alloys. However, comparisons of their positive effects have been rarely presented because their usual fabrication process of annealing is hard to control. In this study, after tuning the composition fluctuation range, self-assembled well-ordered multilayer Mg0.7Gd0.3 films with an excessive amount of nanosized crystals were fabricated by deviating substrates to the edge of the sample stage, while relatively low crystallinity was gained at the center of the sample stage with a small composition fluctuation. It was demonstrated that the hydrogen diffusion rate in the sample deposited at the center with excessive amorphous regions was higher than that of the film fabricated at the edge with excessive nanocrystals regions. Besides, optical conversion ranges were monitored before and after the hydrogen absorption process. Films with large composition fluctuation possessed a high optical reflectance conversion range because the interference effects occurring inside improved their initial reflectance. However, films with small composition fluctuation inside gained a large transmittance conversion range due to their small nanostructured region.
ABSTRACT
Objective: To investigate the immune regulation effect of Ganoderma polysaccharide capsule (GPC) on immunosuppressed mice. Methods: Sixty mice were randomly divided into 6 groups (10 each): normal control group, model group, Lentinan group and 20mg/(kg.d) GPC group, 60mg/(kg.d) GPC group and 180mg/(kg.d) GPC group. The drugs were administrated by intragastric infusion for mice, respectively, once a day for 15 days. From the 13th days, the mice in each group except for control group were injected with cyclophosphamide at a dosage of 80mg/kg, once a day for 3 days. And then the number of peripheral blood cells was counted with veterinary hematology analyzer, the spleen and thymus were weighed and their index were calculated. The T lymphocyte proliferation induced by Con A and LPS was detected with MTT method, NK cytotoxicity was tested with LDH method, carbon clearance was tested by using carbon granules clearance test, and the expressions of IL-2 and IFN-ă mRNA in spleen lymphocytes were detected with RT-PCR. Results: Peripheral blood WBC in 180mg/(kg.d) GPC group, peripheral blood PLT in 60mg/(kg.d) group, the spleen index in 20mg/(kg.d) and 60mg/(kg.d) groups, and thymus index in 20mg/(kg.d) group were increased remarkably when compared with those in model group (P<0.05). Spleen T lymphocyte proliferation induced by Con A and LPS, spleen NK cytotoxicity and carbon clearance in 20mg/(kg.d), 60mg/(kg.d) and 180mg/(kg.d) GPC groups were increased notably when compared with those in the model group (P<0.05 or P<0.01). The expressions of IFN-ă mRNA in spleen lymphocytes in 20mg/(kg.d) and 180mg/(kg.d) groups increased markedly when compared with that in the model group (P<0.01). Conclusion: GPS may enhance immune function and increase peripheral blood WBC and PLT in immunosuppressed mice.
