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Zhong Yao Cai ; 29(2): 179-80, 2006 Feb.
Article in Chinese | MEDLINE | ID: mdl-16617789

ABSTRACT

OBJECTIVE: To prepare and purify polyclonal antibody against chymopapain, and to make a foundation for establishing an immunossay for chymopapain. METHODS: New Zealand rabbit was immunized with chymopapain. Antiserum was purified by Protein A and analyzed by ELISA. RESULTS: The titer of the antiserum obtained in this experiment by ELISA was up to 1:380000 and the purity was proved to be high by SDS-PAGE.


Subject(s)
Antibodies/isolation & purification , Asimina/chemistry , Chymopapain/immunology , Animals , Antibodies/immunology , Antibody Specificity , Chymopapain/chemistry , Enzyme-Linked Immunosorbent Assay , Male , Papain/immunology , Papain/isolation & purification , Rabbits
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