ABSTRACT
Arthrospira maxima is a natural source of fine chemicals for multiple biotechnological applications. We determined the optimal environmental conditions for A. maxima by measuring its relative growth rate (RGR), pigment yield, and photosynthetic performance under different pH and temperature conditions. RGR was highest at pH 7-9 and 30 °C. Chlorophyll a, phycocyanin, maximal quantum yield (Fv/Fm), relative maximal electron transport rate (rETRmax), and effective quantum yield (ΦPSII) were highest at pH 7-8 and 25 °C. Interestingly, phycoerythrin and allophycocyanin content was highest at 15 °C, which may be the lowest optimum temperature reported for phycobiliprotein production in the Arthrospira species. A threestep purification of phycocyanin (PC) by ultrafiltration, ion-exchange chromatography, and gel filtration resulted in a 97.6% purity of PC.
ABSTRACT
The aim of this study was to evaluatethe effects of different colored light emitting diodes (LEDs) on the growth, pigment yield, and photosynthetic performance of Arthrospira maxima, a commercially exploited species of cyanobacteria. The highest growth and chlorophyll a (Chl a) concentration were obtained under red LED and white fluorescent light, while the lowest growth and Chlaconcentration were observed under blue LED. However, blue LED produced the highest levels of phycobiliproteins (3.20â¯mg·g-1phycocyanin [PC]; 0.19â¯mg·g-1 allophycocyanin [APC]; 0.97â¯mg·g-1for phycoerythrin [PE], effective quantum yield (ΦPSII) and maximum relative electron transport rate (rETRmax) inA. maxima. The results of this study suggest that red and blue LEDs increase the biomass yield and pigment content of cyanobacteria, respectively, and the combined use of red and blue light may significantly improve algal biomass and biopigment yield.
