Investigating demic versus cultural diffusion and sex bias in the spread of Austronesian languages in Vietnam.

Austronesian (AN) is the second-largest language family in the world, particularly widespread in Island Southeast Asia (ISEA) and Oceania. In Mainland Southeast Asia (MSEA), groups speaking these languages are concentrated in the highlands of Vietnam. However, our knowledge of the spread of AN-speaking populations in MSEA remains limited; in particular, it is not clear if AN languages were spread by demic or cultural diffusion. In this study, we present and analyze new data consisting of complete mitogenomes from 369 individuals and 847 Y-chromosomal single nucleotide polymorphisms (SNPs) from 170 individuals from all five Vietnamese Austronesian groups (VN-AN) and five neighboring Vietnamese Austroasiatic groups (VN-AA). We found genetic signals consistent with matrilocality in some, but not all, of the VN-AN groups. Population affinity analyses indicated connections between the AN-speaking Giarai and certain Taiwanese AN groups (Rukai, Paiwan, and Bunun). However, overall, there were closer genetic affinities between VN-AN groups and neighboring VN-AA groups, suggesting language shifts. Our study provides insights into the genetic structure of AN-speaking communities in MSEA, characterized by some contact with Taiwan and language shift in neighboring groups, indicating that the expansion of AN speakers in MSEA was a combination of cultural and demic diffusion.

Ovarian hormones in relation to naturally cycling women's conception risk: Empirical evidence and implications for behavioral endocrinology.

A substantial body of literature has examined how women's psychology and behavior vary as a function of conception risk across the ovarian cycle. These effects are widely believed to be outcomes of hormonal regulation, in particular effects of estrogens (E) and progesterone (P). Increasingly, researchers have sought to test predictions about how psychological processes or behavior vary as a function of conception risk by examining associations with estrogen (e.g., estradiol) and progesterone levels. Yet issues regarding how best to assess these associations arise. Should hormone levels be log-transformed? Do hormone ratios best capture their joint effects? How important are hormone interactions? How should outliers be treated? Across two large datasets, we examined hormonal predictors of conception risk, estimated from day of a luteinizing hormone (LH) surge. Log-transformed E and P levels predicted conception risk better than raw E and P levels did. The raw E/P ratio was a relatively poor predictor, whereas the log-transformed ratio (ln[E/P]) was a relatively good predictor. E × P interactions were detected but weak. Outliers were frequent, especially in distributions of raw hormone levels. Hormone measures predicted two psychological outcomes in these datasets-sexual desire and preferences for strength and muscularity-in parallel to how strongly they predicted conception risk. These results give rise to several recommendations regarding treatment of hormone measures and their use in analyses.

Women's Estrus and Extended Sexuality: Reflections on Empirical Patterns and Fundamental Theoretical Issues.

How do women's sexual interests change across their ovulatory cycles? This question is one of the most enduring within the human evolutionary behavioral sciences. Yet definitive, agreed-upon answers remain elusive. One empirical pattern appears to be robust: Women experience greater levels of sexual desire and interest when conceptive during their cycles. But this pattern is not straightforward or self-explanatory. We lay out multiple possible, broad explanations for it. Based on selectionist reasoning, we argue that the conditions that give rise to sexual interests during conceptive and non-conceptive phases are likely to differ. Because conceptive and non-conceptive sex have distinct functions, the sexual interests during conceptive and non-conceptive phases are likely to have different strategic ends. We discuss provisional evidence consistent with this perspective. But the exact nature of women's dual sexuality, if it exists, remains unclear. Additional empirical research is needed. But perhaps more crucially, this topic demands additional theory that fruitfully guides and interprets future empirical research.

Death Anxiety in the Iranian Elderly: A Systematic Review and Meta-Analysis Study.

The present study aimed to estimate the standard score of death anxiety in the Iranian elderly. In this systematic review and meta-analysis, national and international databases were searched from inception to January 2022. All the observational studies published in Persian and English, which reported a death anxiety score based on the Templer death anxiety scale, were selected. The standard score of death anxiety in the elderly was 50% (95% CI: 45-56). No correlation was observed between the year of publication (p = .092) and sample size (p = .94) with the standard score of death anxiety in the elderly. With increased age, death anxiety scores declined. Publication bias was not significant (p = .340). Iranian seniors achieved half of the total score of death anxiety, which indicated the average level of death anxiety. Providing training to effectively manage death anxiety is essential to preventing the adverse consequences of extreme death anxiety.

Cockayne syndrome without UV-sensitivity in Vietnamese siblings with novel ERCC8 variants.

Cockayne syndrome (CS) is a rare progeroid disorder characterized by growth failure, microcephaly, photosensitivity, and premature aging, mainly arising from biallelic ERCC8 (CS-A) or ERCC6 (CS-B) variants. In this study we describe siblings suffering from classical Cockayne syndrome but without photosensitivity, which delayed a clinical diagnosis for 16 years. By whole-exome sequencing we identified the two novel compound heterozygous ERCC8 variants c.370_371del (p.L124Efs*15) and c.484G>C (p.G162R). The causality of the ERCC8 variants, of which one results in a frameshift and the other affects the WD3 domain, was tested and confirmed by a rescue experiment investigating DNA repair in H2O2 treated patient fibroblasts. Structural modeling of the p.G162R variant indicates effects on protein-protein interaction. This case shows the importance to test for ERCC6 and ERCC8 variants even if patients do not present with a complete CS phenotype.

EEG Peak Detection in Cognitive Conflict Processing Using Summit Navigator and Clustering-Based Ranking.

Correct detection of peaks in electroencephalogram (EEG) signals is of essence due to the significant correlation of those potentials with cognitive performance and disorders. This paper proposes a novel and non-parametric approach to detect prediction error negativity (PEN) in cognitive conflict processing. The PEN candidates are first located from the input signal via an adaptation of a recent effective method for local maxima extraction, processed in a multi-scale manner. The found candidates are then fused and ranked based on their shape and location-based features. False positives caused by candidates' magnitude are eliminated by rotating the sorted candidate list where the one with the second-best ranking score will be identified as PEN. The EEG data collected from a 3D object selection task have been used to verify the efficacy of the proposed approach. Compared with the state-of-the-art peak detection techniques, the proposed method shows an improvement of at least 2.67% in accuracy and 6.27% in sensitivity while requires only about 4 ms to process an epoch. The accuracy and computational efficiency of the proposed technique in the detection of PEN in cognitive conflict processing would lead to promising applications in performance improvement of brain-computer interfaces (BCIs).

Spectrum of Genetic Variants in a Cohort of 37 Laterality Defect Cases.

Laterality defects are defined by the perturbed left-right arrangement of organs in the body, occurring in a syndromal or isolated fashion. In humans, primary ciliary dyskinesia (PCD) is a frequent underlying condition of defective left-right patterning, where ciliary motility defects also result in reduced airway clearance, frequent respiratory infections, and infertility. Non-motile cilia dysfunction and dysfunction of non-ciliary genes can also result in disturbances of the left-right body axis. Despite long-lasting genetic research, identification of gene mutations responsible for left-right patterning has remained surprisingly low. Here, we used whole-exome sequencing with Copy Number Variation (CNV) analysis to delineate the underlying molecular cause in 35 mainly consanguineous families with laterality defects. We identified causative gene variants in 14 families with a majority of mutations detected in genes previously associated with PCD, including two small homozygous CNVs. None of the patients were previously clinically diagnosed with PCD, underlining the importance of genetic diagnostics for PCD diagnosis and adequate clinical management. Identified variants in non-PCD-associated genes included variants in PKD1L1 and PIFO, suggesting that dysfunction of these genes results in laterality defects in humans. Furthermore, we detected candidate variants in GJA1 and ACVR2B possibly associated with situs inversus. The low mutation detection rate of this study, in line with other previously published studies, points toward the possibility of non-coding genetic variants, putative genetic mosaicism, epigenetic, or environmental effects promoting laterality defects.

Conductive Gels: Properties and Applications of Nanoelectronics.

Conductive gels are a special class of soft materials. They harness the 3D micro/nanostructures of gels with the electrical and optical properties of semiconductors, producing excellent novel attributes, like the formation of an intricate network of conducting micro/nanostructures that facilitates the easy movement of charge carriers. Conductive gels encompass interesting properties, like adhesion, porosity, swelling, and good mechanical properties compared to those of bulk conducting polymers. The porous structure of the gels allows the easy diffusion of ions and molecules and the swelling nature provides an effective interface between molecular chains and solution phases, whereas good mechanical properties enable their practical applications. Due to these excellent assets, conductive gels are promising candidates for applications like energy conversion and storage, sensors, medical and biodevices, actuators, superhydrophobic coatings, etc. Conductive gels offer promising applications, e.g., as soft sensors, energy storage, and wearable electronics. Hydrogels with ionic species have some potential in this area. However, they suffer from dehydration due to evaporation when exposed to the air which limits their applications and lifespan. In addition to conductive polymers and organic charge transfer complexes, there is another class of organic matter called "conductive gels" that are used in the organic nanoelectronics industry. The main features of this family of organic materials include controllable photoluminescence, use in photon upconversion technology, and storage of optical energy and its conversion into electricity. Various parameters change the electronic and optical behaviors of these materials, which can be changed by controlling some of the structural and chemical parameters of conductive gels, their electronic and optical behaviors depending on the applications. If the conjugated molecules with π bonds come together spontaneously, in a relative order, to form non-covalent bonds, they form a gel-like structure that has photoluminescence properties. The reason for this is the possibility of excitation of highest occupied molecular orbital level electrons of these molecules due to the collision of landing photons and their transfer to the lowest unoccupied molecular orbital level. This property can be used in various nanoelectronic applications such as field-effect organic transistors, organic solar cells, and sensors to detect explosives. In this paper, the general introduction of conductive or conjugated gels with π bonds is discussed and some of the physical issues surrounding electron excitation due to incident radiation and the mobility of charge carriers, the position, and role of conductive gels in each of these applications are discussed.

Psychometric Assessment of the Persian Translation of the Interpersonal Mindfulness Scale With Undergraduate Students.

Interpersonal mindfulness is a construct that significantly contributes to social interaction. To date, no validated measure assessing interpersonal mindfulness has been developed in Iran. Therefore, the aim of this study was to translate and validate the Interpersonal Mindfulness Scale (IMS) among Iranian undergraduate students. Participants in the study (370 undergraduate students; 220 females) from the Azad University completed the translated IMS, the Five Facet Mindfulness Questionnaire, and the Inventory of Interpersonal Problems Scale. The translated measure demonstrated acceptable face validity. All items had acceptable content validity and were deemed essential to the scale. The results of a Confirmatory Factor Analysis (CFA) confirmed a scale with four subscales (presence, awareness of self and others, non-judgmental acceptance, and non-reactivity), with acceptable internal consistency. The findings support the psychometric properties of the Persian translated Interpersonal Mindfulness Scale, which could be used to measure interpersonal mindfulness among undergraduate students in Iran.

Robust evidence for moderation of ovulatory shifts by partner attractiveness in Arslan et al.'s (2020) data.

Arslan et al. (2020) conducted a large-scale, preregistered daily diary study on over 400 normally ovulating women. Of core interest were hypotheses that women's ratings of their partner's sexual attractiveness moderate associations of fertility status with women's own extrapair sexual desires, their own interest in in-pair sex, and their partners' mate retention tactics. The authors claim that "no evidence for moderator effects" (p. 426) was found. In fact, their own analyses reported in their supplementary material show robust evidence for moderation effects. Moreover, a new reanalysis using a more comprehensive composite measure of male partner sexual attractiveness yielded even stronger results. Effect size estimates are consistent with the existence of large, meaningful moderation effects, revealing that this study actually does show evidence of moderation effects. Additional analyses show similarly strong moderator effects on male proprietariness. We discuss the findings in the context of reliance on binary (significant vs. nonsignificant) labels and the pitfalls of underreporting effects. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Leptospira infection among human-close-contact animals in different geographical areas in Vietnam.

Leptospirosis is a neglected disease in Vietnam. Only a few studies have evaluated the status of Leptospira infection in both humans and animals. To our knowledge, this is the first serological survey of Leptospira in both domestic and wild animals, which may act as reservoirs of this agent. This study aimed to evaluate the seroprevalence of Leptospira in animals that are in close contact with humans in different geographical areas in Vietnam. Sera were collected from 1205 individual animals of six species, including buffaloes, cattle, cats, dogs, swine, and rats. The microscopic agglutination test (MAT) against 25 serovars of Leptospira spp. has been employed to detect serovars of Leptospira among the studied population. Overall, 44.2% of buffaloes, 24.9% of cattle, 10.2% of swine, 32.9% of dogs, 12.2% of cats, and 16% of rats were seropositive. A total of 17 different serovars were detected, of which serovars Hebdomadis and Canicola circulated in all the studied animal species. Variability of the predominant serovars circulating in animal species and in different geographical areas of Vietnam has been noted. We conclude that this study showed a high prevalence of Leptospira circulating in animals that are in close contact with humans, raising an alert of the important sources of pathogenic leptospires transmission to humans in Vietnam. These findings prove an imperative need for effective measures for disease prevention.

Identification of DAXX As A Restriction Factor Of SARS-CoV-2 Through A CRISPR/Cas9 Screen

Interferon restricts SARS-CoV-2 replication in cell culture, but only a handful of Interferon Stimulated Genes with antiviral activity against SARS-CoV-2 have been identified. Here, we describe a functional CRISPR/Cas9 screen aiming at identifying SARS-CoV-2 restriction factors. We identified DAXX, a scaffold protein residing in PML nuclear bodies known to limit the replication of DNA viruses and retroviruses, as a potent inhibitor of SARS-CoV-2 and SARS-CoV replication in human cells. Basal expression of DAXX was sufficient to limit the replication of SARS-CoV-2, and DAXX over-expression further restricted infection. In contrast with most of its previously described antiviral activities, DAXX-mediated restriction of SARS-CoV-2 was independent of the SUMOylation pathway. SARS-CoV-2 infection triggered the re-localization of DAXX to cytoplasmic sites and promoted its degradation. Mechanistically, this process was mediated by the viral papain-like protease (PLpro) and the proteasome. Together, these results demonstrate that DAXX restricts SARS-CoV-2, which in turn has evolved a mechanism to counteract its action.

Host PDZ-containing proteins targeted by SARS-Cov-2

Small linear motif targeting protein interacting domains called PDZ have been identified at the C-terminus of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins E, 3a, and N. Using a high-throughput approach of affinity-profiling against the full human PDZome, we identified sixteen human PDZ binders of SARS-CoV-2 proteins E, 3A and N showing significant interactions with dissociation constants values ranging from 3 M to 82 M. Six of them (TJP1, PTPN13, HTRA1, PARD3, MLLT4, LNX2) are also recognized by SARS-CoV while three (NHERF1, MAST2, RADIL) are specific to SARS-CoV-2 E protein. Most of these SARS-CoV-2 protein partners are involved in cellular junctions/polarity and could be also linked to evasion mechanisms of the immune responses during viral infection. Seven of the PDZ-containing proteins among binders of the SARS-CoV-2 proteins E, 3a or N affect significantly viral replication under knock-down gene expression in infected cells. This PDZ profiling identifying human proteins potentially targeted by SARS-CoV-2 can help to understand the multifactorial severity of COVID19 and to conceive effective anti-coronaviral agents for therapeutic purposes.

Endocrinological effects of social exclusion and inclusion: Experimental evidence for adaptive regulation of female fecundity.

When current conditions are probabilistically less suitable for successful reproduction than future conditions, females may prevent or delay reproduction until conditions improve. Throughout human evolution, social support was likely crucial to female reproductive success. Women may thus have evolved fertility regulation systems sensitive to cues from the social environment. However, current understanding of how psychological phenomena might affect female ovarian function is limited. In this study, we examined whether cues of reduced social support-social ostracism-impact women's hormone production. Following an in-lab group bonding task, women were randomly assigned to a social exclusion (n = 88) or social inclusion (n = 81) condition. After social exclusion, women with low background levels of social support experienced a decrease in estradiol relative to progesterone. In contrast, socially-included women with low background social support experienced an increase in estradiol relative to progesterone. Hormonal changes in both conditions occurred specifically when women were in their mid-to-late follicular phase, when baseline estradiol is high and progesterone is low. Follow-up analyses revealed that these changes were primarily driven by changes in progesterone, consistent with existing evidence for disruption of ovarian function following adrenal release of follicular-phase progesterone. Results offer support for a potential mechanism by which fecundity could respond adaptively to the loss or lack of social support.

BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2

SARS-CoV-2 infection of human cells is initiated by the binding of the viral Spike protein to its cell-surface receptor ACE2. We conducted a targeted CRISPRi screen to uncover druggable pathways controlling Spike protein binding to human cells. We found that the protein BRD2 is required for ACE2 transcription in human lung epithelial cells and cardiomyocytes, and BRD2 inhibitors currently evaluated in clinical trials potently block endogenous ACE2 expression and SARS-CoV-2 infection of human cells, including those of human nasal epithelia. Moreover, pharmacological BRD2 inhibition with the drug ABBV-744 inhibited SARS-CoV-2 replication in Syrian hamsters. We also found that BRD2 controls transcription of several other genes induced upon SARS-CoV-2 infection, including the interferon response, which in turn regulates the antiviral response. Together, our results pinpoint BRD2 as a potent and essential regulator of the host response to SARS-CoV-2 infection and highlight the potential of BRD2 as a novel therapeutic target for COVID-19.

The impact of using genetically modified (GM) corn/maize in Vietnam: Results of the first farm-level survey.

This study assessed the farm-level economic and environmental impacts from the use of genetically modified (GM) corn in Vietnam (resistant to Lepidopteran pests of corn and tolerant to the herbicide glyphosate). It was largely based on a farmer survey conducted in 2018-19. The GM varieties out-performed conventional varieties in terms of yield by +30.4% (+15.2% if the yield comparison is with only the nearest performing equivalent conventional varieties) and reduced the cost of production by between US $26.47 per ha and US $31.30 per ha. For every extra US $1 spent on GM seed relative to conventional seed, farmers gained between an additional US $6.84 and US $12.55 in extra income. The GM maize technology also reduced insecticide and herbicide use. The average amount of herbicide active ingredient applied to the GM crop area was 26% lower (1.66 kg per ha) than the average value for the conventional corn area (2.26 kg/ai per ha) and in terms of the associated environmental impact of the herbicide use, as measured by the Environmental Impact Quotient (EIQ) indicator, it was lower by 36% than the average value applicable to the conventional corn area. Insecticides were used on a significantly lower GM crop area and, when used, in smaller amounts. The average amount of insecticide applied to the GM corn crop was significantly lower by 78% (0.08 kg/ai per ha) than the average value for the conventional corn area (0.36 kg/ai per ha) and in terms of the associated environmental impact of the insecticide use, as measured by the EIQ indicator, it was also lower by 77% than the average value for conventional corn (14.06 per ha).

Variations in Antimicrobial Activities of Human Monocyte-Derived Macrophage and Their Associations With Tuberculosis Clinical Manifestations.

Macrophages play a significant role in preventing infection through antimicrobial activities, particularly acidification, and proteolysis. Mycobacterium tuberculosis (Mtb) infection can lead to diverse outcomes, from latent asymptomatic infection to active disease involving multiple organs. Monocyte-derived macrophage is one of the main cell types accumulating in lungs following Mtb infection. The variation of intracellular activities of monocyte-derived macrophages in humans and the influence of these activities on the tuberculosis (TB) spectrum are not well understood. By exploiting ligand-specific bead-based assays, we investigated macrophage antimicrobial activities real-time in healthy volunteers (n = 53) with 35 cases of latent TB (LTB), and those with active TB (ATB), and either pulmonary TB (PTB, n = 70) or TB meningitis (TBM, n = 77). We found wide person-to-person variations in acidification and proteolytic activities in response to both non-immunogenic IgG and pathogenic ligands comprising trehalose 6,6'-dimycolate (TDM) from Mtb or ß-glucan from Saccharamyces cerevisiase. The variation in the macrophage activities remained similar regardless of stimuli; however, IgG induced stronger acidification activity than immunogenic ligands TDM (P = 10-5, 3 × 10-5 and 0.01 at 30, 60, and 90 min) and ß-glucan (P = 10-4, 3 × 10-4 and 0.04 at 30, 60, and 90 min). Variation in proteolysis activity was slightly higher in LTB than in ATB (CV = 40% in LTB vs. 29% in ATB, P = 0.03). There was no difference in measured antimicrobial activities in response to TDM and bacterial killing in macrophages from LTB and ATB, or from PTB and TBM. Our results indicate that antimicrobial activities of monocyte-derived macrophages vary among individuals and show immunological dependence, but suggest these activities cannot be solely responsible for the control of bacterial replication or dissemination in TB.

Summit Navigator: A Novel Approach for Local Maxima Extraction.

This paper presents a novel method, called the Summit Navigator, to effectively extract local maxima of an image histogram for multi-object segmentation of images. After smoothing with a moving average filter, the obtained histogram is analyzed, based on the data density and distribution to find the best observing location. An observability index for each initial peak is proposed to evaluate if it can be considered as dominant by using the calculated observing location. Recursive algorithms are then developed for peak searching and merging to remove any false detection of peaks that are located on one side of each mode. Experimental results demonstrated the advantages of the proposed approach in terms of accuracy and consistency in different reputable datasets.