Prevalência de agravos em saúde e fatores associados em profissionais de limpeza pública / Prevalence of health problems and associated factors in public cleaning professionals

Objetivo: avaliar a prevalência de agravos em saúde e os fatores associados às alterações somatoscópicas, hematológicas, bioquímicas e parasitológicas nos trabalhadores de limpeza pública. Metodologia: Trata-se de uma pesquisa de campo do tipo descritiva, exploratória, com abordagem quantitativa. Resultados: os dados coletados nessa pesquisa permitem afirmar que a maioria eram do sexo feminino, com idade entre 40 a 59 anos, solteiros, não possuíam hábitos etilistas e tabagista. Não praticavam atividades físicas e consideravam-se estressados. Expunham-se diariamente ao sol e não usavam protetor solar, faziam menos de seis refeições diárias, ingeriam menos de dois litros de agua por dia, se consultavam menos de duas vezes ao ano e não usavam equipamentos de proteção individual, na avaliação somatoscópica, os profissionais de limpeza pública apresentavam peso médio de 70,6 kg e dentição com presença de cáries, dentre os valores hematológicos e bioquímicos apresentaram baixo percentual de Hemácias, Linfocitose e HDL em nível tolerável. Houve prevalência de 33% de infestação parasitária entre os participantes. Conclusão: percebe-se que a saúde do trabalhador deve-se desenvolver por um conjunto de ações de assistência e vigilância, buscando detectar e analisar os fatores determinantes e condicionantes dos agravos relacionados à sua rotina laboral.

Objective: to evaluate the prevalence of health problems and the factors associated with somatoscopic, hematological, biochemical and parasitological changes in public cleaning workers. Methodology: This is a descriptive, exploratory field research with a quantitative approach. Results: the data collected in this research allow us to affirm that the majority were female, aged between 40 and 59 years old, single, did not have alcohol and smoking habits. They did not practice physical activities and considered themselves stressed. They exposed themselves daily to the sun and did not use sunscreen, ate less than six meals a day, drank less than two liters of water per day, consulted less than twice a year and did not use personal protective equipment, in the somatoscopic evaluation, public cleaning professionals had an average weight of 70.6 kg and teething with the presence of cavities, among the hematological and biochemical values they presented a low percentage of red blood cells, lymphocytosis and HDL at a tolerable level. There was a 33% prevalence of parasitic infestation among the participants. Conclusion: it is perceived that the worker's health must be developed through a set of assistance and surveillance actions, seeking to detect and analyze the determining and conditioning factors of the injuries related to their work routine.

ECHOCARDIOGRAPHIC FINDINGS OF BIDIMENSIONAL MODE, M-MODE, AND DOPPLER OF CLINICALLY NORMAL BLACK-RUMPED AGOUTI (DASYPROCTA PRYMNOLOPHA, WAGLER 1831).

The black-rumped agouti ( Dasyprocta prymnolopha , Wagler 1831) is currently under intense ecologic pressure, which has resulted in its disappearance from some regions of Brazil. Echocardiography is widely used in veterinary medicine but it is not yet part of the clinical routine for wild animals. The objective of the present study was to assess the applicability of the echocardiographic exam in nonanesthetized agouti and to establish normal reference values for echocardiographic measurements in bidimensional mode (2D), M-mode, and Doppler for this species, and a lead II electrocardiogram was simultaneously recorded. Twenty agouti were used in this study. All the echocardiographic measurements were positively correlated with weight (P < 0.05), and there were no significant differences between sexes (P > 0.05). Blood flow velocities in the pulmonary and aortic artery ranged from 67.32-71.28 cm/sec and 79.22-101.84 cm/sec, respectively. The isovolumic relaxation time was assessed in all the animals and ranged from 38.5 to 56.6 ms. The maximum value for the nonfused E and A waves and the Et and At waves was 158 beats/min for both. The results obtained for the morphologic and heart hemodynamic measurements can guide future studies and help in the clinical management of these animals in captivity.

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil.

PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied. .