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1.
Dakar Med ; 44(1): 69-75, 1999.
Article in French | MEDLINE | ID: mdl-10797991

ABSTRACT

The aim of this study was to set accurate and reliable methods in the identification of Enterobacteriaceae. In Micro CSB Entero each of a strip with 20 cupules containing dehydrated substrates for biochemical identification of bacterial species. Strips were inoculated with 100 microliters per cupule and incubated. After 18 hours, reagents were added. Baye's theorem was used to validate tests. Reactions from Micro CSB were cleared and easily read. 102 strains of Enterobacteriaceae were identified by MicroCSB--89.3% were correctly identified. 0.9% were identified with other tests 9.8% were incorrectly identified MicroCSB gives many advantages: This method save time, space and is less expensive than the other methods and it gives security for identifying species belonging to the different genera.


Subject(s)
Bacterial Typing Techniques , Enterobacteriaceae/classification , Enterobacteriaceae/metabolism , Reagent Strips , Sensitivity and Specificity
2.
Dakar Med ; 44(1): 76-83, 1999.
Article in French | MEDLINE | ID: mdl-10797992

ABSTRACT

The aim of this study was to set accurate and reliable methods in the identification of streptococcal, enterococcal and staphylococcal species. Micro CSB Strep and Staph system consists each of a strip with cupules containing dehydrated substrates for biochemical identification of bacterial species. Baye's theorem was used to validate tests. Reactions from micromethods were clear and easily read. Identification of 229 strains of streptococci and enterococci was correct for most species with 98.7% species with 99.3% sensitivity. 41 strains of staphylococci were also correctly identified with 85.2% of specificity and 97.68% of sensitivity.


Subject(s)
Bacterial Typing Techniques , Enterococcus/classification , Staphylococcus/classification , Streptococcus/classification , Enterococcus/metabolism , Reagent Strips , Reproducibility of Results , Sensitivity and Specificity , Staphylococcus/growth & development , Staphylococcus/metabolism , Streptococcus/metabolism
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