Quantification of Spontaneous Tail Movement in Zebrafish Embryos Using a Novel Open-Source MATLAB Application.

Recently, the evaluation of spontaneous tail movement (STM) in zebrafish embryos has been proposed as a possible neurotoxicity marker in studies on behavioral changes due to chemical exposure. Currently, automated STM quantification can only be performed using high-cost commercial software. We have developed a simple and reliable MATLAB® script called ZebraSTM that allows the automatic analysis of STM in multiple zebrafish embryos.