ABSTRACT
Obesity is an important healthcare issue caused by abnormally increased adipose tissue because of energy-intake overcoming energy expenditure. Disturbances in the physiological function of adipose tissue mediate the development of diabetes. It is a metabolic disease that results from decreased insulin-levels and/or changes in the insulin action mechanism. Tumor Necrosis Factor-Associated Apoptosis-Inducing Ligand(TRAIL), which is a member of the Tumor Necrosis Factor(TNF)-family with an important role in adipose tissue biology, is included in many studies with its ability to induce apoptosis in cancer cells, but the number of human-studies conducted on the gene related to its protective-role against diabetes and obesity at this level is insufficient. Our study was carried out as a case and control and included three groups (80 diabetic obese, 80 non-diabetic obese, and 80 healthy individuals as the control group). The Real-Time-PZR(RT-qPZR), and DNA Sanger-Sequencing Methods were used for gene expression and gene squences. As a result of the analyses, TRAIL gene expression level was found to be higher in the controls than in the diabetic-obese and non-diabetic-obese group. This change in TRAIL gene expression suggests that TRAIL maybe a protective factor against diabetes. The presence of rs781673405, rs143353036, rs1244378045, rs767450259, rs759369504, rs750556128, and rs369143448 mutations, which was determined with the Sequencing-Method, was shown for the first time in the present study. In addition, it is the first study in which human TRAIL gene-expression and sequencing were performed together. We believe that these data will make an important contribution to the literature.
ABSTRACT
Tumors are formed by various clones developed over a long time. This gives rise to a heterogeneous nature. This heterogeneity is the hardest challenge in the treatment of cancers because it is the main reason for drug resistance. This is a well-known fact in human cancer. Therefore, we have reasoned that if the tumor heterogeneity in canine mammary gland tumors (CMGTs) could be shown by an ex vivo assay, which will be used first time in veterinary oncology practice, this could be used further in clinics. To achieve this, twenty-six patients were included in the study. Tumor tissues were obtained from animals during routine surgery. Tumor cells were isolated and seeded ex vivo. The cells were exposed to anticancer drugs that are clinically used. Seven days after the treatment, chemosensitivity has luminometrically been assayed by ATP-tumor chemosensitivity assay (ATP-TCA). It has clearly been shown that all the tumor tissues have responded to treatment differently, implying that heterogeneity exists in mammary tumors. There has also been found that there was a weak to moderate statistically significant correlation between tumor size and drug index. However, there has been no correlation between drug index and metastasis to lymph nodes. Hyperplasic areas had relatively higher PCNA values. The results of our study demonstrate the heterogeneity in responses to in vitro drugs. Clinical trials based on test results and follow-up studies with large numbers of animals are needed to prove that such chemotherapeutic activity assessment tests can be clinically useful in predicting drug responses in CMGTs.
Subject(s)
Antineoplastic Agents , Dog Diseases , Mammary Neoplasms, Animal , Humans , Dogs , Animals , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Antineoplastic Agents/pharmacology , Antineoplastic Agents/therapeutic use , Adenosine Triphosphate , Mammary Neoplasms, Animal/drug therapy , Dog Diseases/drug therapyABSTRACT
Digital flexion creases are one of the external anatomical landmarks in the hand. In this study, we measured and compared the distances between the digital skin creases (haustra digiti) in the fingers of right-handed and left-handed individuals. We also recorded the digital formulae. No significant difference was detected between left and right-handed females and left and right handed males on distance of skin creases (p>0.05). No difference was detected on haustra digiti of the right handed females their own hands. Females who use their left hand had a significantly longer(p=0.48) distal haustra digiti segment on their right second finger than that of their left hand. However, no significant difference was detected between male individuals on their left and right hand all haustra digiti segments. The rate of hand digital formulae of 3>4>2>5>1 was 78.12% in left-handed females, 66.7% in left-handed males, 54.54% in right-handed females, and 78.57% in right-handed males. Our study has contributed to the literature on the morphological asymmetry of left-handed females.
Los pliegues digitales de flexión constituyen unos de los puntos de referencia anatómicos externos en la mano. En este estudio se midieron y compararon las distancias entre los pliegues digitales de la manos (haustra digiti) de individuos diestros y zurdos. También se registraron las fórmulas digitales. No se detectaron diferencias significativas entre mujeres zurdas y diestras y tampoco entre varones diestros y zurdos en relación a la distancia de los pliegues digitales (p>0,05). No se detectaron diferencias en el haustra digiti de las mujeres diestras. Las mujeres que utilizaban su mano izquierda tenían una diferencia significativamente mayor (p=0,48) en el segmento distal del segundo dedo, comparado con el de la mano derecha. Sin embargo, no se detectaron diferencias significativas entre los individuos de sexo masculino en sus manos izquierda y derecha, a nivel de todos los segmentos de los haustra digiti. La fórmula digital 3>4>2>5>1 se encontró en 78,12% de las mujeres zurdas, en el 66,7% de hombres zurdos, en el 54,54% de mujeres diestras, y en el 78,57% de hombres diestros. Nuestro estudio ha contribuido a la literatura sobre la asimetría morfológica de los pliegues digitales de las mujeres zurdas.
Subject(s)
Humans , Male , Female , Young Adult , Functional Laterality , Hand/anatomy & histology , Fingers/anatomy & histology , Sex CharacteristicsABSTRACT
Aloe Barbadensis, which is a species of Aloe vera, is a popular plant used by the common people and in alternative medicine. This study aimed to analyze the effects of Aloe Barbadensis and soybean oil on liver. For this study Wistar Albino female rats were taken and divided into 3 equal groups; the first group was the control group wherein no treatment was applied, second group in which the dissolved form of A. barbadensis in the soybean oil was applied (25 mg/day), and the third group which only soybean oil was applied (500 mg/day). Biopsy materials were taken from the lobus dexter of the livers of the rats and analyzed with light microscope after the necessary standard processing of histologic slides. Group I demonstrated normal structural characteristics of rat liver. In Group II and Group III, we observed nuclear enlargement, mild increase in chromatin and hydropic degeneration and binucleation in some hepatocytes. Liver histology demonstrated congestion in portal veins, sinusoids and the central veins. Merely in Group III, portal venous congestion and in Group II sinusoidal congestion was evident parenchyma of the liver. Additionally in Group III liver histology demonstrated plasmocyte infiltration in portal areas. Our study showed that using soybean with Aloe Barbadensis is synergystic and increasing each others effects. However we didn't observe mononeuclear infiltrations in Group II, these show antinflammatory effects of Aloe Barbadensis. It is determined that, depending on the used dose of Aloe Barbadensis, the toxic effect can change. If Aloe Barbadensis used very high doses it can have toxic effect on hepatocytes.
Aloe Barbadensis, una especie de Aloe vera, es una planta popular usada por el común de las personas y también en la medicina alternativa. El estudio tuvo como objetivo analizar los efectos del Aloe Barbadensis y aceite de soya en el hígado. Para el estudio se emplearon ratas Wistar hembras Albino y se dividieron en 3 grupos: grupo control I sin tratamiento; grupo II A. barbadensis disuelta en aceite de soja (25 mg / día), y grupo III tratado sólo con aceite de soja (500 mg / día). Fueron extraídas biopsias del lóbulo derecho del hígado de las ratas y luego se analizaron con microscopio de luz. En el grupo I el hígado de las ratas era normal. En los grupos II y III, se observó aumento del tamaño nuclear, leve aumento de la cromatina y degeneración hidrópica y binucleación en algunos hepatocitos. La histología hepática mostró la congestión en las venas porta, sinusoides y las centrales. En el grupo III, la congestión venosa portal y en el Grupo II la congestión sinusoidal fue evidente. Además, el Grupo III reveló infiltración de plasmocitos en áreas portales. El uso de soja con Aloe Barbadensis es sinergista y aumenta cada uno de otros efectos. Infiltraciones mononucleares en el grupo III determinan la reacción inflamatoria. Sin embargo, no observamos infiltración mononuclear en el Grupo II, éste mostró efectos antiinflamatorios de la Aloe Barbadensis. Esto determina que, dependiendo de la dosis usada de Aloe Barbadensis, los efectos tóxicos pueden cambiar. Si es usado en altas dosis Aloe Barbadensis puede producir efectos tóxicos en los hepatocitos.
