Feeding strategy for single-stage deammonification to treat moderate-strength ammonium under low free ammonia conditions.

Single-stage deammonification (SSD) processes have been successfully operated using the step-feeding strategy to treat high-strength NH4+ (>300 mg/L), but often failed to treat moderate-strength NH4+ (100-300 mg/L). Because it is hard to maintain the free ammonia (FA) above 1 mg/L, which is a concentration in which the activity of NO2- oxidizing bacteria (NOB) can be selectively suppressed. In this study, to evaluate the effectiveness of the step-feeding strategy on the long-term stability of treating moderate-strength NH4+, two SSD sequential-batch reactors (SBRs) were operated under one-step feeding and multi-step feeding strategies. The one-step feeding SBR achieved a higher nitrogen removal efficiency (86 %), nitrogen removal rate (0.61 kg/m3/d), and COD removal efficiency (95 %) than the multi-step feeding SBR (73 %, 0.39 kg/m3/d, and 95 %, respectively). This means the appropriate FA to selectively suppress NOB activity was successfully maintained in the one-step feeding SBR (FA > 1 mg/L). Therefore, it the necessary to apply a step feed strategy that can be maintained above FA (1 mg/L) from the start-up of operation to treat moderate-strength NH4+.

Long-term adaptation of two anammox granules with different ratios of Candidatus Brocadia and Candidatus Jettenia under increasing salinity and their application to treat saline wastewater.

Nitrogen removal in saline wastewater is a challenge of the anaerobic ammonium oxidation (anammox) process, which is dominated by freshwater anammox bacteria (FAB). Candidatus Brocadia and Candidatus Jettenia, the most widely used FABs, have been separately applied and evaluated for their ability to treat saline wastewater. To understand the effect of salinity on nitrogen removal capability when they present together in an anammox granule, we compared two anammox granules: GRN1 was evenly dominated by Ca. Brocadia (42 %) and Ca. Jettenia (43 %), while GRN2 was dominated with mostly Ca. Brocadia (90 %) and a small amount of Ca. Jettenia (1 %). Each granule was inoculated into a continuous column reactor to treat artificial wastewater containing 150 mg NH4+-N/L and 150 mg NO2--N/L under increasing saline conditions for 250 days. GRN1 showed superior and more stable nitrogen removal than GRN2 under saline conditions of up to 15 g NaCl/L. Under high-saline conditions, both the granules' sizes decreased (larger GRN1 than GRN2 in initial). The mass percent of Na salt increased (more in GRN2) and mineral contents decreased more in GRN1. High-throughput sequencing for microbial community analysis showed that Planctomycetes in GRN1 (85 %) and GRN2 (92 %) decreased to 14 % and 12 %, respectively. The ratio of Ca. Brocadia and Ca. Jettenia in GRN1 changed to 37 % and 63 %, respectively, whereas the ratio in GRN2 (99 % and 1 %, respectively) did not change. Both salt-adapted granules were applied to the two-stage partial nitritation and anammox (PN/A) process to treat high strength ammonium (400 mg/L) wastewater under high saline condition (15 g NaCl/L). The PN/A process containing GRN1 showed more stable nitrogen removal performance during approximately 100 days of operation. These results suggest that the anammox granules evenly dominated by two FABs, Ca. Brocadia and Ca. Jettenia, would be advantageous to treat high-strength NH4+ wastewater under high-saline conditions.

Simultaneous removal of organic and nitrogenous compounds in mature landfill leachate by a hybrid electro-oxidation-dialysis (EOD) system.

Electrochemical process has been widely applied to eliminate recalcitrant contaminants (i.e., organic and nitrogenous compounds) in landfill leachate. This study aimed to evaluate the performance of a hybrid electro-oxidation-dialysis (EOD) system to minimize organic and nitrogenous compounds through a synergistic process of electrochemical oxidation (EO) and electrodialysis (ED) as well as the dissolved organic matter was characterized in terms of fluorescent component and molecular weight distribution. The EOD was carried out using boron-doped diamond (BDD) and Pt alternately. The results have shown that pH adjustment to acidic conditions is beneficial to EO. At optimal pH (pH 4), BDD-based EO is superior to removing COD and NH4+ up to around 56% and 64%, respectively. During EOD process, the lower current density at 20.83 mA cm-2 is preferred for the recovery of nitrogenous ions (i.e. NH4+ and NO3-), especially for BDD-EOD. In addition, the dominant humic acid-like (HAL) and soluble microbial products-like (SMPL) substances in the mature leachate are mostly degraded to smaller molecules from 105 Da to 103 Da in both EOD processes. Overall, BDD-EOD favours indirect oxidation and has a higher energy consumption efficiency than Pt-EOD induced by direct oxidation for simultaneous removal of organic and nitrogenous compounds. BDD-EOD requires a lower total operation cost of around $2.33/m3 compared to Pt-EOD. It is concluded that the hybrid BDD-EOD process is technically feasible as a powerful pre-treatment approach to mature landfill leachate for refractory organics degradation and nitrogenous nutrients recovery.

Effect of chloride ions on the simultaneous electrodialysis and electrochemical oxidation of mature landfill leachate.

An attempt has been made to improve the treatment efficiency of mature landfill leachate prior to the existing biological treatment. In this study, electrochemical oxidation (EO) was applied as a pre-treatment to remove organic contaminants and was simultaneously combined with electrodialysis (ED) to remove ionic constituents, such as ammonium and phosphate. A laboratory-scale electrochemical reactor was designed by utilizing a carbon graphite anode and a stainless steel cathode and separated by an anion exchange membrane (AEM) and cation exchange membrane (CEM), creating a three-compartment reactor. The oxidation of the organic pollutant would occur in the anodic compartment, while the targeted ammonium and phosphate ions would be migrated and accumulated in the central compartment. The treatment process was performed in a batch recirculation time of 12 h at a constant supplied current of 0.25 A and evaluated by means of the initial leachate pH (i.e., original pH value of 7.85; adjusted pH value of 5.50 and 8.50) and three different initial chloride concentrations. The higher the chloride concentration in the leachate, the higher the removal efficiency, except for total phosphate. The highest chemical oxidation demand (COD) removal was 86.2% (0.88 g W-1 h-1), at an initial leachate pH value of 7.85 with the addition of 2 g L-1 of NaCl. Furthermore, under the same conditions, the ammonium, total phosphate, and chloride removals were 85% (0.44 g W-1 h-1), 89% (0.08 g W-1 h-1), and 83% (0.69 g W-1 h-1), respectively. Also, the concentrated ionic compounds in the central compartment can lower the energy consumption and can possibly be further treated or managed.