ABSTRACT
A study of immunomodulating action of mouse alpha/beta interferon (IFN) was performed in conditions of congenital and experimental thymus-dependent immune deficiency. The action of IFN was assessed in vivo, with IFN injected intraperitoneally. It was shown that IFN did not change killer effect of lymphocytes in the reaction of stem cell inactivation in "nude" mice, but enhanced antibody and rosette formation in the spleen of these animals. In addition, IFN did not change these parameters in the spleen of HRS mice with abnormal differentiation of T lymphocytes, but enhanced antibody and rosette formation in the spleen of mice, thymectomized in old age. It is concluded that the normal function of T-system is essential for the action of IFN.
Subject(s)
Adjuvants, Immunologic , Immunologic Deficiency Syndromes/congenital , Immunologic Deficiency Syndromes/therapy , Interferon Type I/therapeutic use , Thymus Gland/immunology , Animals , Antibody Formation/drug effects , Hematopoietic Stem Cells/drug effects , Hematopoietic Stem Cells/immunology , Immunologic Deficiency Syndromes/immunology , Male , Mice , Mice, Inbred Strains , Rosette Formation , T-Lymphocytes/drug effects , T-Lymphocytes/immunology , Thymus Gland/drug effectsABSTRACT
The role of different T lymphocyte subpopulations in the process of allogeneic haemopoietic stem cell inactivation has been studied. The T lymphocyte subpopulations were fractionated in vivo using the homing properties (to the spleen and lymph nodes) plus sensitivity for ALS in vivo (recirculation properties), for thymectomy, for anti-Thy 1 serum in vitro and some others. The results obtained indicate that not only the killer lymphocytes, that provide stem cell inactivation, but also the suppressor cells, that provide inhibition of inactivation, take part in the reaction for allogeneic stem cells. Killer and suppressor cells differ alternatively in some physiological properties and may be separated for further studying.
Subject(s)
Hematopoietic Stem Cells/immunology , Killer Cells, Natural/immunology , T-Lymphocytes, Regulatory/immunology , T-Lymphocytes/immunology , Animals , Antilymphocyte Serum/immunology , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Rabbits , T-Lymphocytes/radiation effects , T-Lymphocytes/transplantation , Thymectomy , Transplantation, Homologous , Transplantation, IsogeneicSubject(s)
Thymus Gland/immunology , Animals , Bone Marrow Transplantation , Interleukin-2/immunology , Killer Cells, Natural/immunology , Lymphatic Diseases/immunology , Lymphatic Diseases/therapy , Mice , Mice, Nude , Radiation Chimera , Rosette Formation , T-Lymphocytes/immunology , T-Lymphocytes/transplantation , Thymectomy , Transplantation, IsogeneicABSTRACT
Hz1 and M505 are two mutants affected the Kend of H-2 major histocompatibility system in mouse. Inability of M505 to complement Hz1 is considered as an evidence that the same gene is altered by both mutations. The gained antigens of two mutants can cross-react in vivo, and the lost antigenic determinants are not identical as revealed in skin grafting tests with presensitized recipients. The antiserum ASY-0,15, (d X a) anti-i, could differentiate between normal and mutant cells as well as between both mutants in absorption tests. These results show that some haptenic determinants are changed in the Hz1 and M505 mutations together with changes of histocompatibility determinants.
