ABSTRACT
This study reports a simplified, low-cost, easily performed chemico-mechanical isolation method of the islets of Langerhans. The experimental study was carried out on 15 adult pigs. Our method used collagenase and a digestion chamber for islet isolation and yielded 2,240 +/- 613 islets per g treated pancreas after purification. This demonstrates that the method is reliable and can give satisfactory results.
Subject(s)
Islets of Langerhans/cytology , Animals , Cell Separation/economics , Cell Separation/instrumentation , Cell Separation/methods , Evaluation Studies as Topic , Female , SwineABSTRACT
The authors analyzed the expression of some genes involved in the control of T lymphocyte proliferation in a group of healthy elderly subjects. They focused their attention on genes involved in the G(0)/G(1) transition (TK, PCNA, H3, IL2-R) and showed decreased expression in the TK, H3 and IL2-R genes. Using flow cytofluorimetry, delayed transition from the G(0)/G(1) to the S stage was observed.
