ABSTRACT
BACKGROUND: Pathogenic yersiniae (Y. pestis, Y. pseudotuberculosis, Y. enterocolitica) share a virulence plasmid encoding a type three secretion system (T3SS). This T3SS comprises more than 40 constituents. Among these are the transport substrates called Yops (Yersinia outer proteins), the specific Yop chaperones (Sycs), and the Ysc (Yop secretion) proteins which form the transport machinery. The effectors YopO and YopP are encoded on an operon together with SycO, the chaperone of YopO. The characterization of SycO is the focus of this study. RESULTS: We have established the large-scale production of recombinant SycO in its outright form. We confirm that Y. enterocolitica SycO forms homodimers which is typical for Syc chaperones. SycO overproduction in Y. enterocolitica decreases secretion of Yops into the culture supernatant suggesting a regulatory role of SycO in type III secretion. We demonstrate that in vitro SycO interacts with YscM1, a negative regulator of Yop expression in Y. enterocolitica. However, the SycO overproduction phenotype was not mediated by YscM1, YscM2, YopO or YopP as revealed by analysis of isogenic deletion mutants. CONCLUSION: We present evidence that SycO is integrated into the regulatory network of the Yersinia T3SS. Our picture of the Yersinia T3SS interactome is supplemented by identification of the SycO/YscM1 interaction. Further, our results suggest that at least one additional interaction partner of SycO has to be identified.
Subject(s)
Bacterial Proteins/metabolism , Gene Expression Regulation, Bacterial , Molecular Chaperones/metabolism , Transcription Factors/metabolism , Yersinia enterocolitica/physiology , Dimerization , Protein Binding , Protein Transport/genetics , Protein Transport/physiology , Yersinia enterocolitica/genetics , Yersinia enterocolitica/metabolismABSTRACT
Yersinia pestis and the enteropathogenic Yersinia pseudotuberculosis and Yersinia enterocolitica share the virulence-antigen LcrV. Previously, using reverse genetics we have proven that LcrV contributes to the virulence of Y. enterocolitica serotype O:8 by inducing IL-10 via Toll-like receptor 2 (TLR2). However, both the ability of Y. pestis LcrV to activate TLR2 and a possible role of TLR2-dependent IL-10 induction by LcrV in Y. pestis are not yet known. To eliminate interference from additional protein sequences, we produced LcrVs without affinity tags from Y. pestis and from Y. enterocolitica O:8 (LcrVO:8). LcrVO:8 was much more potent in TLR2-activity than Y. pestis LcrV. To analyse the role of TLR2 in plague, we infected both wild-type and TLR2-/- mice subcutaneously with Y. pestis GB. While TLR2-/- mice exhibited lower blood levels of IL-10 (day 2 post-infection) and of the pro-inflammatory cytokines TNF-alpha, IFN-gamma and MCP-1 (day 4) than wild-type mice, there was no significant difference in survival. The low TLR2-activity of Y. pestis LcrV and associated cytokine expression might explain why - in contrast to Y. enterocolitica O:8 infection - TLR2-deficient mice are not more resistant than wild-type mice in a bubonic plague model.
Subject(s)
Antigens, Bacterial/metabolism , Macrophages/immunology , Pore Forming Cytotoxic Proteins/metabolism , Toll-Like Receptor 2/metabolism , Yersinia pestis/pathogenicity , Animals , Antigens, Bacterial/genetics , Cell Line , Cytokines/metabolism , Female , Humans , Mice , Mice, Inbred C57BL , Plague/immunology , Plague/microbiology , Plague/mortality , Plague/physiopathology , Pore Forming Cytotoxic Proteins/genetics , Specific Pathogen-Free Organisms , Toll-Like Receptor 2/genetics , Virulence , Yersinia pestis/genetics , Yersinia pestis/metabolismABSTRACT
Type III secretion systems (T3SSs) are engaged by a broad number of Gram-negative bacteria to inject proteins into host cells. The simultaneous crossing of three biological membranes by these proteins is a phenomenon which is far from being understood mechanistically. However, recent work from several groups has substantially enriched our conception of how proteins travel from the bacterial into the host cell cytosol. Here, we focus on the role of specific T3SS chaperones, the specific ATPase, and the proton motive force in type III secretion.
Subject(s)
Adenosine Triphosphatases/metabolism , Bacterial Proteins/metabolism , Gram-Negative Bacteria/metabolism , Molecular Chaperones/physiology , Proton-Motive Force , Virulence Factors/metabolism , Protein TransportABSTRACT
Yersinia species pathogenic to human benefit from a protein transport machinery, a type three secretion system (T3SS), which enables the bacteria to inject effector proteins into host cells. Several of the transport substrates of the Yersinia T3SS, called Yops (Yersinia outer proteins), are assisted by specific chaperones (Syc for specific Yop chaperone) prior to transport. Yersinia enterocolitica SycD (LcrH in Yersinia pestis and Yersinia pseudotuberculosis) is a chaperone dedicated to the assistance of the translocator proteins YopB and YopD, which are assumed to form a pore in the host cell membrane. In an attempt to make SycD amenable to structural investigations we recombinantly expressed SycD with a hexahistidine tag in Escherichia coli. Combining immobilized nickel affinity chromatography and gel filtration we obtained purified SycD with an exceptional yield of 120mg per liter of culture and homogeneity above 95%. Analytical gel filtration and cross-linking experiments revealed the formation of homodimers in solution. Secondary structure analysis based on circular dichroism suggests that SycD is mainly composed of alpha-helical elements. To prove functionality of purified SycD previously suggested interactions of SycD with Yop secretion protein M2 (YscM2), and low calcium response protein V (LcrV), respectively, were reinvestigated.
