ABSTRACT
A modified version of the Lowry protocol for protein measurement is being introduced in which the increment in color due to copper-protein interactions can be directly equated to the concentration of peptide groups (the CO-NH moieties) present in the sample. The method provides an accurate estimation of protein concentrations in the range of 5-40 micrograms, with a sensitivity similar to that of the original assay procedure. It yields hardly any protein-to-protein variation with simple proteins and eliminates the interference by four compounds that commonly affect the Lowry assay. A numerical factor has been calculated that allows conversion of micromoles of peptide groups into actual micrograms of protein present in the sample.
Subject(s)
Colorimetry/methods , Peptides/analysis , Proteins/analysis , Gelatin/analysis , Indicators and Reagents , Microchemistry/methods , Muramidase/analysis , Plant Proteins/analysis , Sensitivity and Specificity , Serum Albumin, Bovine/analysis , Spectrophotometry/methodsABSTRACT
A method for the simultaneous staining of proteins during polyacrylamide gel electrophoresis with Coomassie brilliant blue R-250 at pH 2.5 is described. Calf thymus whole histone and cytochrome c were stained by this method and the results obtained were similar to that obtained by staining after electrophoresis.
Subject(s)
Proteins/isolation & purification , Rosaniline Dyes , Animals , Cattle , Cytochrome c Group/analysis , Electrophoresis, Polyacrylamide Gel , Histones/analysisABSTRACT
The effects of aqueous extracts of onion and garlic as well as of garlic oil were studied on the process of blood coagulation and fibrinolysis in vitro. Only onion was found to exhibit anti-coagulant and fibrinolytic activity while garlic extract as well as garlic oil were inactive.
Subject(s)
Blood Coagulation/drug effects , Fibrinolysis/drug effects , Garlic , Plant Extracts/pharmacology , Plants, Medicinal , Humans , In Vitro Techniques , Prothrombin Time , Thrombin/physiologyABSTRACT
Amberlite IR-120, a polystyrene sulphonate type of cation exchanger, equilibrated with A1 3+ ions, has been employed for the fractionation of whole histone. This adsorbent permits the quantitative and reproducible recovery of whole histone in six fractions.
