ABSTRACT
Lines 6.6-2 (B2B2) and 6.15-5 (B2B2), congenic for the major histocompatibility (B) complex with > 99.9% background gene uniformity, were used to examine primary antibody responses to two antigens. In each of two trials, 1 mL of 5% SRBC, a T cell-dependent antigen, or 0.1 mL of Brucella abortus (BA), a T cell-independent antigen, was injected into separate groups of each B genotype aged 3 and 6 wk. Blood samples were taken from the chickens 7 d after immunization. Serum titers (log2) for both total antibody and mercaptoethanol (ME)-sensitive antibody to detect IgG were assayed by microtiter procedures. Least squares analysis of variance and Fisher's protected Least Significant Difference at P < 0.05 were used to evaluate the data. The total anti-SRBC antibody titer was significantly higher in B5B5 chicks than in B2B2 chicks at 4 and 7 wk of age. There was no significant difference in ME sensitive antibody to SRBC. Chicks of the B5B5 genotype also had significantly higher total and IgG antibody titers to BA at both ages than B2B2 chicks. The results indicate that 4- and 7-wk-old B5B5 chicks had a significantly stronger antibody response to SRBC or BA than B2B2 chicks.
Subject(s)
Antibodies, Bacterial/blood , Chickens/genetics , Chickens/immunology , Major Histocompatibility Complex/immunology , Aging/immunology , Analysis of Variance , Animals , Antibodies, Bacterial/immunology , Brucella abortus/immunology , Brucella abortus/isolation & purification , Chickens/blood , Female , Genotype , Immunoglobulin G/blood , Male , Mercaptoethanol/pharmacology , Probability , Time FactorsABSTRACT
In summary, this survey showed that market research is both necessary and feasible: necessary because there is no other way of getting information for health promotion; and feasible because the quality of results is certainly good enough to shape policy and management action. We believe that such techniques should become routine in many other areas of the health service.
