ABSTRACT
EMMPRIN is known to promote tumor invasion through extracellular matrix (ECM) degradation. Here we report that EMMPRIN can regulate melanoma cell adhesion to the ECM through an interaction with ß1 integrin involving kindlin-3. In this study, EMMPRIN knockdown in the human melanoma cell line M10 using siRNA decreased cell invasion and significantly increased cell adhesion and spreading. A morphological change from a round to a spread shape was observed associated with enhanced phalloidin-labelled actin staining. In situ proximity ligation assay and co-immunoprecipitation revealed that EMMPRIN silencing increased the interaction of ß1 integrin with kindlin-3, a focal adhesion protein. This was associated with an increase in ß1 integrin activation and a decrease in the phosphorylation of the downstream integrin kinase FAK. Moreover, the expression at both the transcript and protein level of kindlin-3 and of ß1 integrin was inversely regulated by EMMPRIN. EMMPRIN did not regulate either talin expression or its interaction with ß1 integrin. These results are consistent with our in vivo demonstration that EMMPRIN inhibition increased ß1 integrin activation and its interaction with kindlin-3. To conclude, these findings reveal a new role of EMMPRIN in tumor cell migration through ß1 integrin/kindlin-3-mediated adhesion pathway.
Subject(s)
Basigin/physiology , Cell Adhesion/physiology , Integrin beta1/physiology , Melanoma/pathology , Membrane Proteins/physiology , Neoplasm Proteins/physiology , Skin Neoplasms/pathology , Animals , Basigin/drug effects , Basigin/genetics , Cell Line, Tumor , Cell Shape/physiology , Extracellular Matrix/physiology , Female , Gene Expression Regulation, Neoplastic/drug effects , Gene Knockdown Techniques , Heterografts , Humans , In Vitro Techniques , Melanoma/physiopathology , Mice , Mice, Nude , RNA, Small Interfering/pharmacology , Signal Transduction/physiology , Skin Neoplasms/physiopathologyABSTRACT
Kindlin-3 (FERMT-3) is known to be central in hemostasis and thrombosis control and its deficiency disrupts platelet aggregation and causes Leukocyte Adhesion Deficiency disease. Here we report that Kindlin-3 has a tumor suppressive role in solid cancer. Our present genetic and functional data show that Kindlin-3 is downregulated in several solid tumors by a mechanism involving gene hypermethylation and deletions. In vivo experiments demonstrated that Kindlin-3 knockdown in 2 tumor cell models (breast cancer and melanoma) markedly increases metastasis formation, in accord with the in vitro increase of tumor cell malignant properties. The metastatic phenotype was supported by a mechanism involving alteration in ß3-integrin activation including decreased phosphorylation, interaction with talin and the internalization of its active form leading to less cell attachment and more migration/invasion. These data uncover a novel and unexpected tumor suppressor role of Kindlin-3 which can influence integrins targeted therapies development.
Subject(s)
Breast Neoplasms/pathology , Genes, Tumor Suppressor , Melanoma/pathology , Membrane Proteins/genetics , Neoplasm Proteins/genetics , Animals , Azacitidine/analogs & derivatives , Azacitidine/pharmacology , Breast Neoplasms/drug therapy , Breast Neoplasms/genetics , Cell Adhesion/genetics , Cell Line, Tumor , Cell Proliferation/genetics , DNA Methylation , Decitabine , Female , Gene Expression Regulation, Neoplastic , Heterografts , Humans , Integrin beta3/metabolism , Melanoma/drug therapy , Melanoma/genetics , Membrane Proteins/biosynthesis , Membrane Proteins/metabolism , Mice , Neoplasm Invasiveness/genetics , Neoplasm Metastasis/genetics , Neoplasm Proteins/biosynthesis , Neoplasm Proteins/metabolism , Neoplasm Transplantation , Phosphorylation , RNA Interference , RNA, Messenger/biosynthesis , RNA, Small Interfering , Talin/geneticsABSTRACT
Type III collagen binding protein (TIIICBP) was previously described as a platelet membrane protein that recognizes the KOGEOGPK peptide sequence within type III collagen. In order to better characterize this protein, we performed different approaches including mass spectrometry sequencing and functional experiments. This study leads to identify high biochemical and functional similarities between TIIICBP and kindlin-3, a member of a family of focal adhesion proteins. Indeed, mass spectrometry surveys indicated that TIIICBP contains several peptides identical to kindlin-3, covering 41% of the amino acid sequence. Polyclonal antibodies raised against a kindlin-3 specific N-terminal sequence, recognized and immunoprecipitated TIIICBP from platelet lysates. Electron microscopy and flow cytometry experiments showed that kindlin-3, as well as TIIICBP, were present associated to platelet membrane and a translocation of cytosolic kindlin-3 to the platelet membrane was observed after platelet activation. Similarly to anti-TIIICBP antibodies and the KOGEOGPK peptide, anti-kindlin-3 antibodies inhibited platelet interactions with type III collagen under flow conditions and slowed down platelet aggregation induced by glycoprotein VI agonists; e.g. collagen-related peptides and convulxin. In addition, the anti-kindlin-3 antibody inhibited platelet aggregation induced by low - but not high - doses of ADP or thrombin which depends on α(IIb)ß(3) integrin function. In conclusion, our results show that the peptides identified by mass spectrometry from purified TIIICBP correspond to the kindlin-3 protein and demonstrate biochemical and functional similarities between TIIICBP and kindlin-3, strengthening a key role for TIIICBP/kindlin-3 in platelet interactions with collagen by cooperating with glycoprotein VI activation and integrin clustering in focal adhesion complexes.
Subject(s)
Blood Platelets/metabolism , Collagen Type III/metabolism , Membrane Proteins/metabolism , Neoplasm Proteins/metabolism , Receptors, Collagen/metabolism , Adenosine Diphosphate/pharmacology , Amino Acid Sequence , Antibodies/metabolism , Blood Platelets/drug effects , Crotalid Venoms/pharmacology , Humans , Immunoprecipitation , Lectins, C-Type , Membrane Proteins/chemistry , Molecular Sequence Data , Neoplasm Proteins/chemistry , Platelet Activation/drug effects , Platelet Adhesiveness/drug effects , Platelet Aggregation/drug effects , Platelet Membrane Glycoproteins/metabolism , Receptors, Collagen/chemistry , Sequence Homology, Amino Acid , Thrombin/pharmacologyABSTRACT
This work aimed to investigate the role of the disintegrin domain of the human ADAM9 (ADAM9D) on the adhesion of breast tumor cells and platelets to collagen I, in a dynamic flow assay to simulate in vivo shear conditions. Recombinant ADAM9D was able to support tumor cell adhesion through binding to the beta1 integrin subunit and also to inhibit the invasion through matrigel in vitro. In a dynamic flow assay ADAM9D inhibited about 75% and 65% of MDA-MB-231 tumor cells and platelet adhesion to collagen I, respectively. In addition, it was demonstrated that alphaVbeta3 integrin is new interacting partner for ADAM9D. In conclusion, these results suggest a role for the disintegrin domain of ADAM9 in the metastatic process. Also, ADAM9D may be a tool for investigating the role of ADAMs in metastasis and cancer progression and for the design of selective inhibitors against the adhesion and extravasation of cancer cells.
Subject(s)
ADAM Proteins/chemistry , ADAM Proteins/pharmacology , Blood Platelets/drug effects , Blood Platelets/metabolism , Collagen Type I/metabolism , Disintegrins/metabolism , Membrane Proteins/chemistry , Membrane Proteins/pharmacology , ADAM Proteins/genetics , ADAM Proteins/isolation & purification , Animals , Cell Adhesion/drug effects , Cell Line, Tumor , Cloning, Molecular , Collagen/metabolism , Drug Combinations , Endothelium/metabolism , Humans , Integrin alphaVbeta3/metabolism , Laminin/metabolism , Membrane Proteins/genetics , Membrane Proteins/isolation & purification , Protein Structure, Tertiary , Proteoglycans/metabolism , RatsABSTRACT
We report the synthesis of collagen related peptides containing the peptide sequence Lys-Hyp-Gly-Glu-Hyp-Gly-Pro-Lys. The anti-thrombotic activity effects of different glycine mutations in this sequence were studied in regard with their different adopted conformations. The biological results could be correlated to the glycine propensity to adopt a more stable polyproline II helix conformation. The incorporation of these sequences in "collagen-like" alpha-triple-helix peptides shows a pro-thrombotic activity compared to a scrambled negative control peptide which possesses no significant activity.
