ABSTRACT
The phytochemical investigation of the DCM/MeOH (1:1) extract of the fruits of Cnestis ferruginea led to the isolation and characterization of one new quinic acid derivative, ferruginoic acid (1), together with six known compounds 2-7. Compounds 3-7 were reported for the first time from this species. The structures of compounds 1-7 were elucidated on the basis of 1 D and 2 D NMR spectroscopic data, mass spectrometry and by comparison of spectroscopic data with those from the literature. The anticholinesterase (AChE and BChE) activity and DPPH free radical scavenging assay of compounds 1, 3, 4 and 7 were evaluated. Ferruginoic acid (1) exhibited moderate anticholinesterase activity with IC50 value of 36.18 ± 1.78 µg/mL against AChE. Compounds 3, 4 and 7 showed high activity against free radical (DPPHâ¢) scavenging assay (DPPH) with IC50 values 40.09 ± 0.96 µg/mL, 61.70 ± 0.78 µg/mL and 41.87 ± 0.62 µg/mL respectively. These results indicate that C. ferruginea and its constituents could be employed in the management of Alzheimer's disease.
Subject(s)
Connaraceae , Connaraceae/chemistry , Cholinesterase Inhibitors/chemistry , Fruit , Plant Extracts/pharmacology , Plant Extracts/chemistry , Pain , Free Radicals , Antioxidants/pharmacologyABSTRACT
One new indole alkaloid derivative, 5,6-dioxo-11-hydroxy voacangine (1) together with four known compounds (2-5), were isolated from the fruits of Tabernaemontana inconspicua Stapf (Apocynaceae). Their structures were determined using 1D and 2D NMR, HRESI-MS, and a comparison with the literature. The new compound was found to be cytotoxic on human breast cancer MDA-MB 231 cells with IC50 values of 3.35 µM and 2.19 µM after 24 and 48 hours, respectively.
Subject(s)
Indole Alkaloids/pharmacology , Tabernaemontana/chemistry , Carbon-13 Magnetic Resonance Spectroscopy , Cell Death/drug effects , Cell Line, Tumor , Humans , Indole Alkaloids/chemistry , Proton Magnetic Resonance Spectroscopy , StereoisomerismABSTRACT
A new lactam, oligoamide (1), along with three known compounds (2-4), stigmasterol-3-O-ß-D-glucopyranoside (2), formononetin (3) and (-)-pinitol (4) were isolated from the CH2Cl2/CH3OH (1:1) extract of the leaves of Angylocalyx oligophyllus by chromatographic separation. Their structures were elucidated on the basis of spectroscopic analysis (UV, IR, MS, 1D, and 2D NMR). Compound 1 was found to have weak antioxidant and urease inhibitory potential.
Subject(s)
Fabaceae/chemistry , Lactams/isolation & purification , Plant Leaves/chemistry , Antioxidants/chemistry , Antioxidants/isolation & purification , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/isolation & purification , Enzyme Inhibitors/pharmacology , Lactams/chemistry , Molecular Structure , Spectrum Analysis , Urease/antagonists & inhibitorsABSTRACT
Chemical investigation of Aspergillus japonicus CAM231, isolated from the leaf of Garcina preussii collected in Cameroon, yielded two new compounds; one pyrone derivative, hydroxy neovasinin (1) and one phenol derivative, asperolan (2), together with two known compounds neovasifurarone B (3) and variecolin (4). The structures of the two new compounds were established using intensive NMR spectroscopy and HRMS spectra in comparison with data found in literature. The structure of compound 1 was confirmed by single-crystal X-ray crystallographic analysis in combination with NOESY experiment. The new compounds were screened for their cytotoxic and antibacterial properties; however, the tested compounds displayed no significant activities.
