Seeds of Hyoscyamus muticus L. subsp. falezlez: Morpho-anatomical Features, Phytochemical Investigation and Evidence for Antioxidant Activities.

This work aims to describe the morpho-anatomy and determine the mineral composition of H. muticus L. subsp. falezlez seed, and investigate the influence of extraction solvents (hexane, petroleum ether, chloroform and methanol) on contents of total phenolic (TPC) and flavonoid (TFC) and the antioxidant activity (DPPH, ABTS, reducing power and phenanthroline). The mineral content analysis revealed the presence of different elements. The results indicated that the seed extract obtained by methanol exhibited the highest yield and TPC (82.07%, 27.14 ± 2.2 µg GAE/mg), while the chloroform extract had the maximum TFC (24.63 ± 0.4 µg QE/mg). In addition, all seed extracts showed excellent performances on DPPH scavenging activity. However, methanolic extract was the most effective extraction solvent for scavenging ABTS free radicals (IC50 <12.5 µg/mL). Chloroform and petroleum ether extracts were the most potent in phenanthroline activity. GC-MS analysis identified 27 phytochemical compounds in all extracts and 16 compounds were identified by HPLC/DAD analysis of the methanolic extract. The multivariate analysis results highlighted the influence of solvents on the phenolic composition and antioxidant activity of seed extracts. The results suggest that H. muticus L. seeds can be a promising source enriched with bioactive constituents for application in the pharmaceutical industry.

Phlomis crinita Cav. From Algeria: A source of bioactive compounds possessing antioxidant and wound healing activities.

ETHNOPHARMACOLOGICAL RELEVANCE: Phlomis crinita Cav. (Lamiaceae), locally known as "El Khayata" or "Kayat El Adjarah", is traditionally used in Algeria for its wound-healing properties. AIM OF THE STUDY: Investigate, for the first time, the phytochemical profile, safety, antioxidant and wound-healing activities of the flowering tops methanolic extract of P. crinita (PCME) collected from Bouira Province in the North of Algeria. MATERIALS AND METHODS: Preliminary phytochemical assays were carried out on PCME to quantify the main classes of bioactive compounds, such as total phenols, flavonoids, and tannins. An in-depth LC-DAD-ESI-MS analysis was carried out to elucidate the phytochemical profile of this plant species. Antioxidant activity was investigated by several colorimetric and fluorimetric assays (DPPH, TEAC, FRAP, ORAC, ß-carotene bleaching and ferrozine assay). The acute oral toxicity of PCME (2000 mg/kg b.w.) was tested in vivo on Swiss albino mice, whereas the acute dermal toxicity and wound-healing properties of the PCME ointment (1-5% PCMO) were tested in vivo on Wistar albino rats. Biochemical and histological analyses were carried out on biological samples. RESULTS: The phytochemical screening highlighted a high content of phenolic compounds (175.49 ± 0.8 mg of gallic acid equivalents/g of dry extract), mainly flavonoids (82.28 ± 0.44 mg of quercetin equivalents/g of dry extract). Fifty-seven compounds were identified by LC-DAD-ESI-MS analysis, belonging mainly to the class of flavones (32.27%), with luteolin 7-(6″-acetylglucoside) as the most abundant compound and phenolic acids (32.54%), with salvianolic acid C as the most abundant compound. A conspicuous presence of phenylethanoids (15.26%) was also found, of which the major constituent is forsythoside B. PCME showed a strong antioxidant activity with half-inhibitory activity (IC50) ranging from 1.88 to 37.88 µg/mL and a moderate iron chelating activity (IC50 327.44 µg/mL). PCME appears to be safe with Lethal Dose 50 (LD50) ≥ 2000 mg/kg b.w. No mortality or toxicity signs, including any statistically significant changes in body weight gain and relative organs' weight with respect to the control group, were recorded. A significant (p < 0.001) wound contraction was observed in the 5% PCMO-treated group with respect to the untreated and petroleum jelly groups between 8 and 20 days, whereas no statistically significant results were observed at the two lower doses (1 and 2% PCMO). In addition, the 5% PCMO-treated group showed a statistically significant (p < 0.05) wound healing activity with respect to the reference drug-treated group, showing, at the end of the study, the highest wound contraction percentage (88.00 ± 0.16%). CONCLUSION: PCME was safe and showed strong antioxidant and wound-healing properties, suggesting new interesting pharmaceutical applications for P. crinita based on its traditional use.

High perillaldehyde content from essential oils of Ammodaucus leucotrichus subsp. leucotrichus Cosson & Durieu fruits from Aougrout (Algeria).

The chemical composition of the essential oil produced by hydrodistillation from the fruits of Ammodaucus leucotrichus subsp. leucotrichus Cosson & Durieu collected from southern Algeria, precisely in Aougrout District (Adrar Province), was determined by GC-MS analysis. Nineteen (19) compounds, representing 85.71% of the essential oil, were found. The essential oil contained a high percentage of perillaldehyde (80.69%). This composition is compared to numerous reported essential oils of A. leucotrichus subsp. leucotrichus fruits that are found across Algeria and Morocco in various regions. The major component perillaldehyde has drawn a lot of interest in recent years due to its notable antifungal and broader antimicrobial properties, as well as its potential advantages for human health. Thus, the studied plant represents a source of this component that should be used as food additives, perfume ingredients, and in formulations of traditional medicine.

Phytochemical characterization, in vivo and in vitro biological activities of Atriplex lindleyi Moq. subsp. inflata.

ETHNOPHARMACOLOGICAL RELEVANCE: Atriplex lindleyi Moq. subsp. inflata from Algeria is known for its traditional use for therapeutic properties. AIM OF THE STUDY: We analysed quantitatively and qualitatively the infusion and diethyl ether, n-butanol and ethereal extracts of A. lindleyi, as well as assessed their acute toxicity, anti-inflammatory, antinociceptive and antioxidant activities. MATERIALS AND METHODS: The total phenolic and flavonoid contents were quantified. The anti-inflammatory effect was assessed using a carrageenan-induced paw oedema assay and the antinociceptive effect was evaluated using an acetic acid-induced writhing method. In addition, antioxidant activity was examined by three tests: DPPH, reducing power and phenanthroline assays. RESULTS: The quantity of total phenols in the roots of A. lindleyi was larger than in the aerial parts, while the amounts of total flavonoids were larger in the aerial parts than in the roots. The HPLC profiles allowed us to identify 32 compounds belonging to the phenolic acid and flavonoid classes. Intraperitoneal administration of the infusions and phenolic extracts in mice did not cause any symptoms of toxicity or mortality. Results revealed that the aerial parts and roots of A. lindleyi had potential anti-inflammatory and antinociceptive activities and were higher than diclofenac and paracetamol, respectively. The diethyl ether extract (DEE) of aerial parts and roots showed the greatest antioxidant activity in comparison to the other tested extracts. CONCLUSION: The phenolic composition attempted to be identified by HPLC confers this plant's pharmacological potential. The antioxidant potential may be due to active ingredients, including ascorbic acid, in the two studied parts of A. lindleyi, which is a bioactive molecule with strong antioxidant properties. Also, detecting salicylic acid, the active antinociceptive ingredient of aspirin, gallic and ferulic acids may justify this subspecies' antinociceptive and anti-inflammatory potentials.

Performance of halotolerant bacteria associated with Sahara-inhabiting halophytes Atriplex halimus L. and Lygeum spartum L. ameliorate tomato plant growth and tolerance to saline stress: from selective isolation to genomic analysis of potential determinants.

The use of halotolerant beneficial plant-growth-promoting (PGP) bacteria is considered as a promising eco-friendly approach to improve the salt tolerance of cash crops. One strategy to enhance the possibility of obtaining stress-alleviating bacteria is to screen salt impacted soils. In this study, amongst the 40 endophytic bacteria isolated from the roots of Sahara-inhabiting halophytes Atriplex halimus L. and Lygeum spartum L., 8 showed interesting NaCl tolerance in vitro. Their evaluation, through different tomato plant trials, permitted the isolate IS26 to be distinguished as the most effective seed inoculum for both plant growth promotion and mitigation of salt stress. On the basis of 16S rRNA gene sequence, the isolate was closely related to Stenotrophomonas rhizophila. It was then screened in vitro for multiple PGP traits and the strain-complete genome was sequenced and analysed to further decipher the genomic basis of the putative mechanisms underlying its osmoprotective and plant growth abilities. A remarkable number of genes putatively involved in mechanisms responsible for rhizosphere colonization, plant association, strong competition for nutrients, and the production of important plant growth regulator compounds, such as AIA and spermidine, were highlighted, as were substances protecting against stress, including different osmolytes like trehalose, glucosylglycerol, proline, and glycine betaine. By having genes related to complementary mechanisms of osmosensing, osmoregulation and osmoprotection, the strain confirmed its great capacity to adapt to highly saline environments. Moreover, the presence of various genes potentially related to multiple enzymatic antioxidant processes, able to reduce salt-induced overproduction of ROS, was also detected.

Chemical diversity analysis and biological activities evaluation of different polyphenolic extracts of Marrubium deserti De Noé from Algeria.

ETHNOPHARMACOLOGICAL RELEVANCE: Marrubium deserti De Noé (Lamiaceae) commonly known as desert horehound, is a small perennial herb distributed in arid climates. This plant has been used in Algeria for the treatment of cold, cough, fever and inflammation. AIM OF THE STUDY: This study aimed to perform a phytochemical analysis and to evaluate the effect of diethyl ether (ExDiEth), n-butanol (ExBut) and hydroalcoholic (ExHyD) extracts of the aerial parts of M. deserti De Noé on inflammation, nociceptive response and fever in animal models. MATERIALS AND METHODS: The total phenolic content and the different phenolic fraction (flavones-flavonols, free aglycones, anthocyanidins, C-glycosides and flavonic heterosides) were quantified in order to study the chemical diversity. The anti-inflammatory effect was evaluated using carrageenan-induced paw oedema test and the nociceptive response was determined by the chemical method using acetic acid-induced writhing response and formalin-induced paw licking tests and by the thermal method using the hot plate in mice. In addition, anti-fever was investigated using yeast-induced pyrexia in rats. RESULTS: Quantitative analysis revealed that among all extracts, ExBut had a higher phenolic content and the aerial parts of M. deserti De Noé collected from two different sites (Biskra and Tamanresset) had a significant difference in the contents of flavonic heterosids and flavones-flavonols. PCA and CA showed a strong intra-specific variability of M. deserti De Noé individuals. Our results showed that the ExDiEth, ExBut and ExHyD treatments prevented the increase of paw oedema by 56.25%, 66.65% and 45.56% inhibition percent, respectively. Moreover, treatments with these extracts significantly (p<0.05) decreased the number of writhing and increased the reaction time to thermal stimulation as well as demonstrated reduction in paw licking time both at early and late phases. Likewise, they also showed a significant reduction of yeast-induced pyrexia till 4 h of treatment. CONCLUSION: Results from this study show that M. deserti De Noé aerial parts exhibited anti-inflammatory, analgesic with central and peripheral actions and antipyretic activities which could be associated with the presence of some phytochemicals as flavones, flavonols, flavonic heterosids and phenolic acids.

Actinobacteria Associated with Vineyard Soils of Algeria: Classification, Antifungal Potential Against Grapevine Trunk Pathogens and Plant Growth-Promoting Features.

Grapevine trunk diseases (GTDs) are among the most destructive diseases of vineyards worldwide, including Algeria. In the fungal complex involved in GTD symptoms, referred as grapevine trunk-pathogens, Paeomoniella chlamydospora and Phaeoacremonium minimum have a determining infecting role as pioneer fungi. Due to the lack of efficiency of conventional disease management practices, a search for alternative strategies, such as biocontrol, is needed. Taking the approach of looking for biocontrol candidates in the environment surrounding the plant, the present study explored actinobacteria diversity within vineyard soils of six grape-producing regions in Algeria. Based on their 16S rRNA gene sequence, identification and phylogenic analysis were performed on the 40 isolates of actinobacteria obtained. Forty percent of strains were attached to Streptomyces, including two evidenced new species, and 32.5% were affiliated to Saccharothrix. The other less represented genera were Actinoplanes, Nocardia, Nocardiopsis, Lentzea, Nonomuraea, Promicromonospora, Saccharopolyspora and Streptosporangium. Screening based on antagonistic and plant growth promotion (PGP) abilities of the strains showed that 47.5% of the isolates exhibited appreciable antagonistic activities against both Pa. chlamydospora and Pm. minimum, with the two best strains being Streptomyces sp. Ms18 and Streptomyces sp. Sb11. Screening for plant growth promoting properties demonstrated that majority of the strains were able to produce indole acetic acid, siderophores, ammonia, ACC deaminase, cellulase and amylase, and fix N2. Through a PGP-traits-based cluster analysis, the most interesting strains were highlighted. Taking into account both antagonistic and PGP properties, Streptomyces sp Sb11 was selected as the most promising candidate for further evaluations of its efficiency in a GTDs context.