Effect of compost, nitrogen salts, and NPK fertilizers on methane oxidation potential at different temperatures.

The effects of compost, nitrogen salts, and nitrogen-phosphorous-potassium (NPK) fertilizers on the methane oxidation potential (MOP) of landfill cover soil at various temperatures were assessed. For this, we used batch assays conducted at 5°C, 15°C, and 25°C with microcosms containing landfill cover soil slurries amended with these elements. Results indicated variable impacts dependent on the type of amendment and the incubation temperature. For a given incubation temperature, MOP varied from one compost to another and with the amount of compost added, except for the shrimp/peat compost. With this latter compost, independent of the amount, MOP values remained similar and were significantly higher than those obtained with other composts. Amendment with most of the tested nitrogen salts led to similar improvements in methanotrophic activity, except for urea. MOP with NPK fertilizer addition was amongst the highest in this study; the minimum value obtained with NPK (20-0-20) suggested the importance of P for methanotrophs. MOP generally increased with temperature, and nutrient limitation became less important at higher temperatures. Overall, at each of the three temperatures tested, MOP with NPK fertilizer amendments provided the best results and was comparable to those observed with the addition of the shrimp/peat compost. The results of this study provide the first evidence of the following: (1) compost addition to improve methanotrophic activity in a landfill cover soil should consider the amount and type of compost used and (2) the importance of using NPK fertilizers rather than nitrogen salts, in enhancing this activity, primarily at low temperatures. One can also consider the potential beneficial impact of adding these elements to enhance plant growth, which is an advantage for MOP.

Faecal coliforms and faecal streptococci community in the underground water in an equatorial area in Cameroon (Central Africa): the importance of some environmental chemical factors.

A bacteriological and chemical study was carried out within 1 year on spring and well water of Yaounde. It assessed the importance of some chemical factors on some faecal bacterial communities. The monthly average densities of faecal coliforms and faecal streptococci varied, respectively, from 1 to 72 x 10(2) CFU 100 ml(-1), and from 1 to 31 x 10(2) CFU 100 ml(-1) of water. These bacterial abundances undergo spatio-temporal fluctuations. These spring and well waters which are slightly bicarbonate, are acidic, soft, with low to average mineralisation. Most of the chemical characteristics of these waters are relatively stable with time, with respect to apparent spatial fluctuations. The degree of correlation between chemical parameters and the abundance dynamics of isolated bacteria is heterogeneous. In an artificially constituted spring biotope, the increase in pH, electrical conductivity, concentration of dissolved oxygen, chloride, sodium, potassium, calcium and magnesium favoured (P<0.01) the abundance of faecal coliforms and faecal streptococci. The high concentration of dissolved CO2 reduces (P<0.001) the abundances of these bacteria. They would nevertheless have developed such a mechanism allowing to minimise inhibitory effects of some environmental factors. In the well water, the ecology of faecal streptococci and faecal coliforms undergoes a relative instability, probably due to the variability of the resultant interaction network.