Use of Moringa oleifera seed extracts to polish effluents from natural systems treating faecal sludge.

The removal of pathogens in irrigation water is of great importance in developing countries. Indeed, wastewater generally reused for agriculture in countries such as Cameroon is associated with health and environmental concerns. Recent studies have shown a strong disinfectant action of the natural coagulant from the seeds of Moringa oleifera. These findings have raised the question whether or not they can be used to polish effluents from natural systems treating faecal sludge. This paper deals with trials carried out to investigate the effect of these extracts in reducing faecal indicators from initially treated faecal sludge leachate. Bacteriological and physico-chemical parameters were used to determine the optimum conditions and assess treatment efficacy. Settling time of 3 h at a concentration of extracts between 267 and 333 mg/L permitted to reduce Escherichia coli and faecal coliforms from 4.85 to 3.92 ulog (86.74%) and from 5.75 to 4.87 ulog (86.39%) respectively with 1 ulog equal to 90%. For the same settling time and at a concentration of 333 mg/L, faecal streptococci were removed from 6.40 to 5.67 ulog (81.33%). This level of removal suggests that this natural coagulant cannot be used alone for disinfection of heavily loaded effluent. Further investigations are therefore still needed to fulfil the Cameroon and WHO guidelines for safe reuse in agriculture.

Floristic surveys of hydrocarbon-polluted sites in some Cameroonian cities (Central Africa).

Soils pollution with hydrocarbons is on the increase, especially in developing countries like Cameroon. Identifying plant species capable of growing on hydrocarbon-polluted soils remains an essential step in phytoremediation, a low cost, solar energy driven clean up technique in which living green plants are used. Here, we report floristic surveys conducted in 4 cities of Cameroon to identify plants with phytoremediation capacities. A total of 13 hydrocarbon-polluted sites were surveyed in each city using the quadrat method. Unpolluted sites served as the control. 106 species belonging to 76 genera and 30 families were identified on hydrocarbon-polluted sites. Plant diversity was shown to be higher (166 species contained in 125 genera and 50 families) in the control sites. Poaceae, Cyperaceae, Asteraceae and Amaranthaceae families have a higher taxonomic richness on polluted sites (16, 15, 10 and 8 taxa respectively). Shannon diversity index of the hydrocarbon-polluted sites (1.6 to 2.7 bits/ind.) were significantly lower than the control sites (2.7 to 3.2 bit/ind.). Based on a relative frequency > 10% and abundance > 7%, our results show that more than 15 plant species could be tested in preliminary trials to determine their phytoremediation/cleaning-up capacities on hydrocarbon-polluted soils in the tropics.