[Effect of the conditions of preparing erythrocyte ghosts on their radiosensitivity]. / Vliianie uslovii polucheniia tenei éritrotsitov na ikh radiochuvstvitel'nost'.

Conditions are chosen in which radiosensitive and radioresistant erythrocyte ghost preparations could be obtained: this may be valuable in studying mechanisms of the distinctions observed.

[Mechanism of change in the fluorescence parameters of pyrene and diphenylhexatriene in irradiated membranes]. / Issledovanie mekhanizmov izmeneniia parametrov fluorestsentsii pirena i difenilgeksatriena v obluchennykh membranakh.

The erythrocyte ghosts were irradiated with doses of 100 to 1000 Gy. The fluorescence intensity and the lifetime of the excited state of pyrene and diphenylhexatriene were shown to decrease. The analysis of the results obtained has demonstrated that the changes in the fluorescence parameters of these probes are related to the enhanced dynamic probe quenching, the quencher being placed in water or the water itself being a quencher.

[Inductive-resonance energy transfer between chromophores localized in different parts of irradiated and nonirradiated erythrocyte ghosts]. / Induktivno-rezonansnyi perenos énergii mezhdu khromoforami, lokalizovannymi v raznykh uchastkakh obluchennykh i neobluchennykh tenei éritrotsitov.

A study was made of the inductive-resonance energy transfer between chromophore pairs, tryptophan--pyren, tryptophan--1.8-anilinonaphthalene sulfonate (ANS), puren--1.8-ANS, diphenylhexatrien--ethidium, and 1,8-ANS--ethidium, in irradiated (250 Gy) and nonirradiated preparations of erythrocytic membranes. The radiation--induced decrease in the energy transfer was noted in the pairs in which one of the chromophores was localized in a lipid phase. On the basis of the data obtained it is suggested that irradiation of membranes causes the lesions in them which lead to a reduction of the effective thickness of a hydrophobic part of the lipid bilayer.