Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Vopr Virusol ; 39(6): 245-7, 1994.
Article in Russian | MEDLINE | ID: mdl-7716914

ABSTRACT

The authors present the results of studies on the construction of highly sensitive primers for rapid diagnosis of infections caused by Herpes simplex virus (HSV) and cytomegalovirus. An oligonucleotide primer to HSV-1 is described, which permits detection of HSV-1 DNA in infected cultures and in clinical material in dilution 10(-7), whereas the "universal" primer constructed on the basis of published data detects both HSV-1 and HSV-2 only in dilution 10(-4). Study of the clinical material in polymerase chain reaction (PCR) vs. virus isolation in infected cell cultures permitted us assess the diagnostic value of this reaction with the constructed primers as high. Use of two pairs of primers (those designed to HSV-1 and the "universal") permitted a differential diagnosis in the PCR between HSV-1 and HSV-2 in clinical material. Highly specific oligonucleotide primers were designed for the rapid diagnosis of cytomegaloviral infection, and clinical material was investigated making use of these primers.


Subject(s)
Cytomegalovirus Infections/diagnosis , DNA Primers , Herpes Genitalis/diagnosis , Herpes Simplex/diagnosis , Base Sequence , Cells, Cultured , Cytomegalovirus/genetics , DNA, Viral/analysis , Diagnosis, Differential , Herpesvirus 1, Human/genetics , Herpesvirus 2, Human/genetics , Humans , Molecular Sequence Data , Polymerase Chain Reaction
SELECTION OF CITATIONS
SEARCH DETAIL
...