Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 22
Filter
Add more filters










Publication year range
1.
Brain Res ; 1675: 8-19, 2017 Nov 15.
Article in English | MEDLINE | ID: mdl-28855102

ABSTRACT

Ethanol and age-induced pathologies of the Purkinje neuron (PN) may result from histone deacetylases (HDACs), enzymes which repress transcription through coiling of the DNA. The purposes of this study were to investigate expression patterns of Class 1 and IIa HDACs in PN and the effects of aging and alcohol on the density of HDACs and histone acetylation in PN. Ninety, eight month old rats (30/diet) were fed a liquid ethanol, liquid control, or rat chow diet for 10, 20, or 40weeks (30/treatment duration). Double immunocytochemical labeling on tissue sections from these rats used antibodies against HDAC isoforms or acetylated histones, and calbindin, a marker for PN. Fluorescent intensities were also measured. Results showed a significant age but not an alcohol-related decrease in the densities of HDACs 2, 3, and 7. In contrast, there were age related-increases in the densities of phosphorylated form of HDAC (4, 5, 7) PN and in PN nuclei expressing HDAC 7. There were also a trend towards ethanol-induced inhibition of acetylation as the density of AH2b PN nuclei and AH3 and AH2b fluorescent intensity was significantly lower in the EF compared to the PF rats. This study presents unique data concerning which HDACs are commonly expressed in PN and indicates that aging rather than lengthy alcohol expression alters expression of the HDACs studied here. These results also suggest that lengthy ethanol consumption may inhibit histone deacetylation in PN.


Subject(s)
Aging/metabolism , Ethanol/administration & dosage , Gene Expression Regulation, Enzymologic , Histone Deacetylases/biosynthesis , Purkinje Cells/enzymology , Aging/drug effects , Alcohol Drinking/metabolism , Alcohol Drinking/pathology , Animals , Histone Deacetylases/genetics , Male , Purkinje Cells/drug effects , Purkinje Cells/pathology , Rats , Rats, Inbred F344
2.
Cerebellum ; 14(4): 466-73, 2015 Aug.
Article in English | MEDLINE | ID: mdl-25648753

ABSTRACT

Uncomplicated alcoholics suffer from discrete motor dysfunctions that become more pronounced with age. These deficits involve the structure and function of Purkinje neurons (PN), the sole output neurons from the cerebellar cortex. This review focuses on alterations to the PN dendritic arbor in the adult and aging Fischer 344 rat following lengthy alcohol consumption. It describes seminal studies using the Golgi-Cox method which proposed a model for ethanol-induced dendritic regression. Subsequent ultrastructural studies of PN dendrites showed dilation of the extensive smooth endoplasmic reticulum (SER) which preceded and accompanied dendritic regression. The component of the SER that was most affected by ethanol was the sarco/endoplasmic reticulum Ca(2+) ATPase pump (SERCA) responsible for resequestration of calcium into the SER. Ethanol-induced decreases in SERCA pump levels, similar to the finding of SER dilation, preceded and occurred concomitantly with dendritic regression. Discrete ethanol-induced deficits in balance also accompanied these decreases. Ethanol-induced ER stress within the SER of PN dendrites was proposed as an underlying cause of dendritic regression. It was recently shown that increased activation of caspase 12, inherent to the ER, occurred in PN of acute slices in ethanol-fed rats and was most pronounced following 40 weeks of ethanol treatment. These findings shed new light into alcohol-induced disruption in PN dendrites providing a new model for the discrete but critical changes in motor function in aging, adult alcoholics.


Subject(s)
Aging/physiology , Central Nervous System Depressants/pharmacology , Dendrites/drug effects , Ethanol/pharmacology , Purkinje Cells/ultrastructure , Animals , Cerebellar Cortex/cytology , Purkinje Cells/drug effects , Rats
3.
Neuropsychopharmacology ; 40(4): 893-905, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25284318

ABSTRACT

Prenatal ethanol exposure (PE) is one of the developmental factors leading to increased addiction propensity (risk). However, the neuronal mechanisms underlying this effect remain unknown. We examined whether increased excitatory synaptic transmission in ventral tegmental area (VTA) dopamine (DA) neurons, which is associated with drug addiction, was impacted by PE. Pregnant rats were exposed to ethanol (0 or 6 g/kg/day) via intragastric intubation from gestational day 8-20. Amphetamine self-administration, whole-cell recordings, and electron microscopy were performed in male offspring between 2 and 12-week-old. The results showed enhanced amphetamine self-administration in PE animals. In PE animals, we observed a persistent augmentation in calcium-permeable AMPA receptor (CP-AMPAR) expression, indicated by increased rectification and reduced decay time of AMPAR-mediated excitatory postsynaptic currents (AMPAR-EPSCs), enhanced depression of AMPAR-EPSCs by NASPM (a selective CP-AMPAR antagonist), and increased GluA3 subunits in VTA DA neuron dendrites. Increased CP-AMPAR expression in PE animals led to enhanced excitatory synaptic strength and the induction of CP-AMPAR-dependent long-term potentiation (LTP), an anti-Hebbian form of LTP. These observations suggest that, in PE animals, increased excitatory synaptic strength in VTA DA neurons might be susceptible to further strengthening even in the absence of impulse flow. The PE-induced persistent increase in CP-AMPAR expression, the resulting enhancement in excitatory synaptic strength, and CP-AMPAR-dependent LTP are similar to effects observed after repeated exposure to drugs of abuse, conditions known to increase addiction risk. Therefore, these mechanisms could be important neuronal substrates underlying PE-induced enhancement in amphetamine self-administration and increased addiction risk in individuals with fetal alcohol spectrum disorders.


Subject(s)
Central Nervous System Depressants/toxicity , Dopaminergic Neurons/physiology , Ethanol/toxicity , Excitatory Postsynaptic Potentials/physiology , Prenatal Exposure Delayed Effects/pathology , Ventral Tegmental Area/pathology , Adrenergic Agents/administration & dosage , Amphetamine/administration & dosage , Analysis of Variance , Animals , Biophysics , Dopaminergic Neurons/drug effects , Dopaminergic Neurons/ultrastructure , Electric Stimulation , Excitatory Postsynaptic Potentials/drug effects , Female , In Vitro Techniques , Male , Microscopy, Electron, Transmission , Patch-Clamp Techniques , Pregnancy , Prenatal Exposure Delayed Effects/chemically induced , Rats , Rats, Sprague-Dawley , Receptors, AMPA/metabolism , Self Administration , Synapses/ultrastructure , Tyrosine 3-Monooxygenase/metabolism , Ventral Tegmental Area/drug effects
4.
Brain Res ; 1577: 11-20, 2014 Aug 19.
Article in English | MEDLINE | ID: mdl-24976582

ABSTRACT

The purpose of this study was to determine, whether previously reported ethanol-induced alterations to the smooth endoplasmic reticulum (SER), predispose Purkinje neurons (PN) to thapsigargin-induced endoplasmic reticulum (ER) stress. Thapsigargin blocks the sarco/endoplasmic Ca(2+) ATPase pump (SERCA 2), depleting the SER of calcium. Forty-one, eight month old Fischer 344 male rats were treated with either the AIN (American Institute of Nutrition) liquid control or ethanol diets for 10 (n=14), 20 (n=10), or 40(n=17) weeks. At the end of treatment, acute cerebellar slices were prepared by standard means. Cerebellar slices were treated with thapsigargin or as controls for three hours in oxygenated (95% CO2, 5% O2) ACSF (artificial cerebrospinal fluid). Slices were then fixed in 4% paraformaldehyde and sectioned on a freezing microtome. Free floating sections were stained with antibodies against activating transcription factor 6 (ATF6) or activated caspase 12 and calbindin. Results showed a significant increase in the activated caspase+PN dendrites in the EF rats along with a significant interaction due to enhanced expression of activated caspase 12 at 20 weeks. The density of ATF6 labeling was not different between the EF and PF groups and was confined to the PN soma. The finding of activated caspase and ATF6 expression in PN within both the EF and PF groups supports the finding of thapsigargin-induced ER stress. The finding of increased activated caspase 12 in the dendrites supports an increased tendency to ER stress and other dendritic deficits in the ethanol rats.


Subject(s)
Activating Transcription Factor 6/metabolism , Caspase 12/metabolism , Central Nervous System Depressants/administration & dosage , Ethanol/administration & dosage , Purkinje Cells/drug effects , Purkinje Cells/metabolism , Alcohol Drinking/metabolism , Alcohol Drinking/pathology , Animals , Calbindins/metabolism , Dendritic Spines/drug effects , Dendritic Spines/metabolism , Dendritic Spines/pathology , Endoplasmic Reticulum Stress/drug effects , Endoplasmic Reticulum Stress/physiology , Male , Purkinje Cells/pathology , Rats, Inbred F344 , Thapsigargin/toxicity
5.
Alcohol Alcohol ; 48(6): 667-78, 2013.
Article in English | MEDLINE | ID: mdl-23884168

ABSTRACT

UNLABELLED: Chronic ethanol consumption for 40 weeks in adult rats results in dilation of the extensive smooth endoplasmic reticulum (SER), a major component of the calcium homeostatic system within Purkinje neuron (PN) dendrites. AIMS: The aim of the present study was to determine whether chronic ethanol consumption results in alterations of the sarco/endoplasmic reticulum Ca(2+) ATPase pump (SERCA) on the SER membrane of PN dendrites. The density of calreticulin, a calcium chaperone, was also investigated in the PN along with balancing ability. METHODS: Ninety 8-month-old rats were exposed to rat chow, the AIN-93 M liquid control or ethanol diets (30/diet) for a duration of 10, 20 or 40 weeks (30/duration). Age changes relative to the rat chow controls were assessed with 3-month-old control rats (n = 10). Balance was assessed prior to euthanasia. Quantitative immunocytochemistry was used to determine the density of SERCA 2b + dendrites and calreticulin + PN soma and nuclei. Molecular layer volumes were also determined. RESULTS: Following 40 weeks of ethanol treatment, there were ethanol-induced decreases in SERCA 2b densities within the dendritic arbor and decreased balancing ability on the more difficult round rod balance test. There were no ethanol-induced changes in calreticulin densities. CONCLUSION: It can be concluded that ethanol-induced decreases in the SERCA pump accompany SER dilation and contribute to previously reported ethanol-induced dendritic regression in PN. Ethanol-induced changes in balance also occurred. Chronic ethanol consumption does not alter calreticulin expression in PN.


Subject(s)
Behavior, Animal/drug effects , Calreticulin/biosynthesis , Central Nervous System Depressants/pharmacology , Ethanol/pharmacology , Purkinje Cells/drug effects , Purkinje Cells/metabolism , Sarcoplasmic Reticulum Calcium-Transporting ATPases/biosynthesis , Aging/metabolism , Animals , Body Weight/drug effects , Brain/drug effects , Calcium/metabolism , Central Nervous System Depressants/blood , Cerebellum/drug effects , Dendrites/metabolism , Diet , Ethanol/blood , Homeostasis/physiology , Immunohistochemistry , Male , Neuroglia/metabolism , Organ Size/drug effects , Rats , Rats, Inbred F344
6.
PLoS One ; 8(6): e67473, 2013.
Article in English | MEDLINE | ID: mdl-23840713

ABSTRACT

UNLABELLED: Pyruvate dehydrogenase (PDH) complex (PDC) deficiency is an inborn error of pyruvate metabolism causing a variety of neurologic manifestations. Systematic analyses of development of affected brain structures and the cellular processes responsible for their impairment have not been performed due to the lack of an animal model for PDC deficiency. METHODS: In the present study we investigated a murine model of systemic PDC deficiency by interrupting the X-linked Pdha1 gene encoding the α subunit of PDH to study its role on brain development and behavioral studies. RESULTS: Male embryos died prenatally but heterozygous females were born. PDC activity was reduced in the brain and other tissues in female progeny compared to age-matched control females. Immunohistochemical analysis of several brain regions showed that approximately 40% of cells were PDH(-). The oxidation of glucose to CO2 and incorporation of glucose-carbon into fatty acids were reduced in brain slices from 15 day-old PDC-deficient females. Histological analyses showed alterations in several structures in white and gray matters in 35 day-old PDC-deficient females. Reduction in total cell number and reduced dendritic arbors in Purkinje neurons were observed in PDC-deficient females. Furthermore, cell proliferation, migration and differentiation into neurons by newly generated cells were reduced in the affected females during pre- and postnatal periods. PDC-deficient mice had normal locomotor activity in a novel environment but displayed decreased startle responses to loud noises and there was evidence of abnormal pre-pulse inhibition of the startle reflex. CONCLUSIONS: The results show that a reduction in glucose metabolism resulting in deficit in energy production and fatty acid biosynthesis impairs cellular differentiation and brain development in PDC-deficient mice.


Subject(s)
Brain Diseases/pathology , Brain/abnormalities , Disease Models, Animal , Pyruvate Dehydrogenase Complex Deficiency Disease/complications , Pyruvate Dehydrogenase Complex/physiology , Animals , Brain/metabolism , Brain/pathology , Brain Diseases/etiology , Carbohydrate Metabolism , Female , Lipogenesis/physiology , Male , Mice , Mice, Knockout , Pyruvate Dehydrogenase Complex Deficiency Disease/physiopathology
7.
Alcohol ; 45(1): 11-8, 2011 Feb.
Article in English | MEDLINE | ID: mdl-20880661

ABSTRACT

Gender-related differential sensitivity to ethanol has long been recognized. Our previous studies have demonstrated that the zebrafish, an animal model used currently to study genetics and development related to a variety of human diseases, is also sensitive to pharmacologically relevant concentrations of ethanol. Sensitivity to ethanol in the zebrafish can be easily gauged with a simple nonintrusive behavioral test that measures ethanol-related alterations in schooling by determining the distance between each fish and its nearest neighbor. The purpose of this study was to determine the influence of gender on the strain-specific ethanol sensitivity that we had observed previously. One hundred and sixty zebrafish of the wild-type (WT) and the long fin striped (LFS) strains were equally divided by gender for use in this study. For acute ethanol treatment, the fish were separated by gender and strain and exposed to 0.0, 0.125, 0.25 0.50, or 1.0% (vol/vol) ethanol. In the chronic study, eight fish of each strain and gender were exposed to 0.5% (vol/vol) ethanol for a period of 10 weeks and the swimming behavior tested before treatment and after each week of treatment. Results showed that female WT zebrafish displayed enhanced sensitivity to the effects of chronic ethanol exposure of increased nearest neighbor distances, whereas male and female LFS fish were not significantly affected by chronic ethanol exposure. Results of the acute ethanol study showed a dose-dependent effect in both strains and a gender effect that needs to be further investigated before enhanced female sensitivity to acute ethanol can be verified.


Subject(s)
Behavior, Animal/drug effects , Ethanol/pharmacology , Zebrafish/physiology , Animals , Ethanol/administration & dosage , Ethanol/pharmacokinetics , Female , Male , Models, Animal , Sex Characteristics , Species Specificity , Swimming/physiology
8.
Alcohol Clin Exp Res ; 34(6): 1013-21, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20374213

ABSTRACT

BACKGROUND: Fetal alcohol exposure during development results in a host of cardiac abnormalities including atrial and ventricular septal defects, teratology of Fallot, d-transposition of the great arteries, truncus arteriosus communis, and aortico-pulmonary window. The mechanisms behind these ethanol-induced deficits are unknown. The purpose of this study was to determine whether the zebrafish, a simple model in which heart development and the sequence of gene expression is well elucidated and comparable to that in higher vertebrates, is sensitive to developmental exposure of pharmacologically relevant concentrations of ethanol. METHODS: Zebrafish eggs of the AB strain were raised in egg water or in 0.5% (v/v) ethanol solution for either 54 hpf (hours postfertilization) or 72 hpf. Heart pathology and volumes were evaluated on the latter group at 5 dpf (days postfertilization) on tissue sections from fixed larvae embedded in glycolmethacrylate. Heart rates were determined in embryos of 54 hpf and larvae of 5 dpf. The functional maturity of the heart's conducting system was measured by determining the response of ethanol-treated and control embryos and larvae to the adrenergic agonist, isoproterenol, and the cholinergic agonist, carbachol. RESULTS: Ethanol-induced alterations occurred in heart morphology and heart volume. A developmental lag in the isoproterenol response and the absence of carbachol-mediated bradycardia were also observed following ethanol treatment. CONCLUSIONS: These results show that exposure of the zebrafish to ethanol during development results in structural and functional changes in the heart that mimic malformations that occur in patients with fetal alcohol syndrome (FAS). These findings promote the zebrafish heart as a future model for investigating the mechanisms responsible for ethanol's adverse effects on vertebrate heart development.


Subject(s)
Central Nervous System Depressants/pharmacology , Ethanol/pharmacology , Heart/drug effects , Heart/embryology , Adrenergic Agonists/pharmacology , Animals , Carbachol/pharmacology , Cardiac Volume/drug effects , Central Nervous System Depressants/adverse effects , Cholinergic Agonists/pharmacology , Ethanol/adverse effects , Female , Heart/physiology , Heart Rate/drug effects , Heart Rate/physiology , Isoproterenol/pharmacology , Models, Animal , Zebrafish
9.
Invest Ophthalmol Vis Sci ; 51(3): 1728-37, 2010 Mar.
Article in English | MEDLINE | ID: mdl-19834036

ABSTRACT

PURPOSE: Photoreceptor rhodopsin kinase (Rk, G protein-dependent receptor kinase 1 [Grk1]) phosphorylates light-activated opsins and channels them into an inactive complex with visual arrestins. Grk1 deficiency leads to human retinopathy and heightened susceptibility to light-induced photoreceptor cell death in the mouse. The goal of this study was to determine whether excess Grk1 activity is protective against photoreceptor cell death. METHODS: Grk1-overexpressing transgenic mice (Grk1(+)) were generated by using a bacterial artificial chromosome (BAC) construct containing mouse Grk1, along with its flanking sequences. Quantitative reverse transcription-PCR, immunoblot analysis, immunostaining, and activity assays were combined with electrophysiology and morphometric analysis, to evaluate Grk1 overexpression and its effect on physiologic and morphologic retinal integrity. Morphometry and nucleosome release assays measured differences in resistance to photoreceptor cell loss between control and transgenic mice exposed to intense light. RESULTS: Compared with control animals, the Grk1(+) transgenic line had approximately a threefold increase in Grk1 transcript and immunoreactive protein. Phosphorylated opsin immunochemical staining and in vitro phosphorylation assays confirmed proportionately higher Grk1 enzyme activity. Grk1(+) mice retained normal rod function, normal retinal appearance, and lacked evidence of spontaneous apoptosis when reared in cyclic light. In intense light, Grk1(+) mice showed photoreceptor damage, and their susceptibility was more pronounced than that of control mice with prolonged exposure times. CONCLUSIONS: Enhancing visual pigment deactivation does not appear to protect against apoptosis; however, excess flow of opsin into the deactivation pathway may actually increase susceptibility to stress-induced cell death similar to some forms of retinal degeneration.


Subject(s)
G-Protein-Coupled Receptor Kinase 1/genetics , Gene Expression Regulation, Enzymologic/physiology , Radiation Injuries, Experimental/enzymology , Retina/radiation effects , Retinal Degeneration/enzymology , Retinal Rod Photoreceptor Cells/enzymology , Animals , Apoptosis , Cell Survival , Chromosomes, Artificial, Bacterial , Electrophysiology , Female , Fluorescent Antibody Technique, Indirect , Genotype , Immunoblotting , In Situ Nick-End Labeling , Light , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C3H , Mice, Inbred C57BL , Mice, Transgenic , Phosphorylation , RNA, Messenger/metabolism , Radiation Injuries, Experimental/pathology , Retinal Degeneration/pathology , Reverse Transcriptase Polymerase Chain Reaction , Rhodopsin/metabolism
10.
Brain Res ; 1221: 98-107, 2008 Jul 24.
Article in English | MEDLINE | ID: mdl-18559274

ABSTRACT

Chronic ethanol consumption in aging rats results in regression of Purkinje neuron (PN) dendritic arbors ([Pentney, 1995 Measurements of dendritic pathlengths provide evidence that ethanol-induced lengthening of terminal dendritic segments may result from dendritic regression. Alcohol Alcohol. 30, 87-96]), loss of synapses (Dlugos and Pentney, 1997), dilation of the smooth endoplasmic reticulum (SER), and the formation of degenerating bodies within PN dendrites ([Dlugos, C.A., 2006a. Ethanol-Related Smooth Endoplasmic Reticulum Dilation in Purkinje Dendrites of Aging Rats. Alcohol., Clin. Exp. Res. 30, 883-891,Dlugos, C.A., 2006b. Smooth endoplasmic reticulum dilation and degeneration in Purkinje neuron dendrites of aging ethanol-fed female rats. Cerebellum. 5, 155-162]). Dilation of the SER and the formation of degenerating bodies may be a predictor of dendritic regression. Ethanol-induced effects on mitochondria may be involved as mitochondria cooperate with the SER to maintain calcium homeostasis. The purpose of this study was to determine whether degenerating body number and mitochondrial density and structure are altered by chronic ethanol treatment in PN dendrites. Male, Fischer 344 rats, 12 months of age, were fed an ethanol or pair-fed liquid diet, or rat chow for a period of 10, 20, or 40 weeks (15 rats/treatment; 45 rats/treatment duration). Ethanol-fed rats received 35% of their calories as ethanol. At the end of treatment, all animals were euthanized, perfused, and tissue prepared for electron microscopy. The densities of degenerating bodies and mitochondria, mitochondrial areas, and the distance between the SER and the mitochondria were measured. Results showed that there was an ethanol-related increase in degenerating bodies compared to controls at 40 weeks. Ethanol-induced alterations to mitochondria were absent. Correlation of the present results with those of previous studies suggest that degenerating bodies may be formed from membrane reabsorption during dendritic regression or from degenerating SER whose function has been compromised by dilation.


Subject(s)
Aging/pathology , Alcohol-Induced Disorders, Nervous System/pathology , Dendrites/drug effects , Ethanol/toxicity , Nerve Degeneration/chemically induced , Purkinje Cells/drug effects , Alcohol-Induced Disorders, Nervous System/chemically induced , Alcohol-Induced Disorders, Nervous System/physiopathology , Animals , Central Nervous System Depressants/toxicity , Cerebellar Cortex/drug effects , Cerebellar Cortex/pathology , Cerebellar Cortex/physiopathology , Cerebellar Diseases/chemically induced , Cerebellar Diseases/pathology , Cerebellar Diseases/physiopathology , Dendrites/pathology , Disease Models, Animal , Endoplasmic Reticulum, Smooth/drug effects , Endoplasmic Reticulum, Smooth/pathology , Male , Microscopy, Electron, Transmission , Mitochondria/drug effects , Mitochondria/pathology , Nerve Degeneration/pathology , Nerve Degeneration/physiopathology , Purkinje Cells/pathology , Rats , Rats, Inbred F344
11.
Eur J Pharmacol ; 583(1): 40-7, 2008 Mar 31.
Article in English | MEDLINE | ID: mdl-18262520

ABSTRACT

Induction of mRNA or protein for immediate-early genes, such as c-fos, is used to identify brain areas, specific cell types, and neuronal circuits that become activated in response to various stimuli including psychoactive drugs. The objective of the present study was to identify the cell types in the prefrontal cortex in which lysergic acid diethylamide (d-LSD) induces c-Fos expression. Systemic administration of d-LSD resulted in a dose-dependent increase in c-Fos immunoreactivity. Although c-Fos-positive cells were found in all cortical layers, they were most numerous in layers III, IV, and V. d-LSD-induced c-Fos immunoreactivity was found in cells co-labeled with anti-neuron-specific enolase or anti-oligodendrocyte Oligo1. The Oligo1-labeled cells had small, round bodies and nuclear diameters characteristic of oligodendrocytes. Studies using confocal microscopy confirmed colocalization of c-Fos-labeled nuclei in NeuN-labeled neurons. Astrocytes and microglia labeled with glial fibrillary acidic protein antibody and OX-42 antibody, respectively, did not display LSD-induced c-Fos expression. Pyramidal neurons labeled with anti-neurofilament antibody also did not show induction of c-Fos immunoreactivity after systemic d-LSD administration. The present study demonstrates that d-LSD induced expression of c-Fos in the prefrontal cortex occurs in subpopulations of neurons and in oligodendrocytes, but not in pyramidal neurons, astrocytes, and microglia.


Subject(s)
Gene Expression Regulation/drug effects , Genes, fos , Hallucinogens/pharmacology , Lysergic Acid Diethylamide/pharmacology , Oligodendroglia/metabolism , Prefrontal Cortex/metabolism , Animals , Astrocytes/drug effects , Astrocytes/metabolism , Cell Count , Dose-Response Relationship, Drug , Glial Fibrillary Acidic Protein/metabolism , Immunohistochemistry , Male , Microglia/drug effects , Microglia/metabolism , Microscopy, Confocal , Oligodendroglia/drug effects , Oligodendroglia/ultrastructure , Perfusion , Prefrontal Cortex/cytology , Prefrontal Cortex/drug effects , Pyramidal Cells/drug effects , Pyramidal Cells/metabolism , Rats , Rats, Inbred F344
12.
J Comp Neurol ; 502(4): 497-506, 2007 Jun 01.
Article in English | MEDLINE | ID: mdl-17394139

ABSTRACT

Approximately 90% of fetal alcohol syndrome cases are accompanied by ocular abnormalities. The zebrafish (Danio rerio) is a well-known developmental model that provides an opportunity for better understanding the histological and cytological effects of developmental exposure to ethanol on the vertebrate eye. The purpose of the present study was to determine the gross, microscopic, and ultrastructual effects of developmental exposure to ethanol in the zebrafish model. Eggs were obtained from WT outbred zebrafish and exposed to 0%, 0.1%, 0.2%, 0.4%, 0.5%, or 1.0% (v/v) ethanol to assess viability and the effect of dose and duration of exposure on eye size. Light and electron microscopy were performed on ethanol-treated and control larvae. Results showed that ethanol treatment decreased viability by about 20% at concentrations of 0.1-0.5% ethanol and by 50% at 1.0% ethanol. Ethanol-related decreases in eye size were recorded at 6 days postfertilization (dpf) and were dose dependent. There were significant decreases in the volumes of the photoreceptor, inner nuclear, and ganglionic layers and in the lens of 9 dpf ethanol-exposed compared with control larvae. Ultrastructural examination showed signs of developmental lags in the ethanol-treated fish as well as abnormal retinal apoptosis in the 6 dpf ethanol-treated larvae compared with their controls. These results demonstrate that the developing zebrafish eye is sensitive to perturbation with ethanol and displays some of the eye deficits present in fetal alcohol syndrome.


Subject(s)
Alcohol-Induced Disorders, Nervous System/physiopathology , Ethanol/toxicity , Eye Abnormalities/chemically induced , Eye Abnormalities/physiopathology , Eye/drug effects , Eye/physiopathology , Alcohol-Induced Disorders, Nervous System/chemically induced , Alcohol-Induced Disorders, Nervous System/pathology , Animals , Apoptosis/drug effects , Cell Differentiation/drug effects , Cell Differentiation/physiology , Cell Survival/drug effects , Cell Survival/physiology , Central Nervous System Depressants/toxicity , Dose-Response Relationship, Drug , Eye/pathology , Eye Abnormalities/pathology , Female , Male , Microscopy, Electron, Transmission , Neurons/drug effects , Neurons/pathology , Ovum/drug effects , Ovum/pathology , Retina/abnormalities , Retina/drug effects , Retina/physiopathology , Zebrafish
13.
Eur J Pharmacol ; 547(1-3): 75-82, 2006 Oct 10.
Article in English | MEDLINE | ID: mdl-16978605

ABSTRACT

The effects of chronic ethanol treatment on the brain proteome were investigated in the long-fin striped strain of zebrafish Danio rerio. Prolonged exposure to 0.5% (v/v) ethanol resulted in the development of tolerance to the ethanol-induced disruption of normal swimming behavior. This behavioral tolerance was manifested after two weeks of continuous treatment and was maintained for an additional three weeks. After four weeks of ethanol treatment, zebrafish brains were divided into 40,000 g supernatant and pellet fractions, and an Ettan 2-D fluorescence difference gel electrophoresis (DIGE) system was used to detect ethanol-induced alterations in the level of protein expression. Protein identification was carried out using matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry and the Mascot and ProFound search engines. In the present study, we have identified some novel protein targets as well as substantiated some putative previous targets of chronic ethanol exposure.


Subject(s)
Brain/drug effects , Ethanol/pharmacology , Proteomics/methods , Zebrafish Proteins/analysis , Animals , Behavior, Animal/drug effects , Brain/metabolism , Central Nervous System Depressants/pharmacology , Electrophoresis, Gel, Two-Dimensional/methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Swimming/physiology , Time Factors , Zebrafish
14.
Cerebellum ; 5(2): 155-62, 2006.
Article in English | MEDLINE | ID: mdl-16818390

ABSTRACT

The effects of chronic ethanol consumption on the extensive Purkinje neuron (PN) dendritic arbor of male rats include dilation of the smooth endoplasmic reticulum (SER) and dendritic regression. The purpose of the present study was to examine the molecular layer of female rats for the presence of ethanol-related SER dilation and evidence of degeneration within the PN dendritic arbor. Twenty-one 12-month-old Fischer 344 female rats (n = 7/treatment group) received a liquid ethanol, liquid control, or rat chow diet for a period of 40 weeks. Ethanol-fed rats received 35% of their dietary calories as ethanol. Pair-fed rats received a liquid control diet that was isocaloric to the ethanol diet. Chow-fed rats received standard laboratory rat chow ad libitum. At the end of treatment, tissues from the anterior and posterior lobes of the cerebellar vermis were viewed and photographed with the electron microscope. The diameters of SER profiles were measured and the density of degenerating bodies within the PN dendritic arbor was quantitated. In the posterior lobe, ethanol-related SER dilation was apparent. In the anterior lobe, the density of degenerating bodies within PN dendritic shafts was significantly increased but SER dilation in PN dendritic shafts was absent. These results confirm that SER dilation and dendritic degeneration in PN dendrites may precede and contribute to ethanol-related regression in female rats. In addition, comparison of these results with data obtained in male rats from a previous study suggests that PN dendrites in females may be more sensitive to the effects of ethanol.


Subject(s)
Alcohol-Induced Disorders, Nervous System/pathology , Alcohol-Induced Disorders, Nervous System/physiopathology , Dendrites/drug effects , Endoplasmic Reticulum, Smooth/drug effects , Ethanol/toxicity , Purkinje Cells/drug effects , Aging/physiology , Animals , Central Nervous System Depressants/toxicity , Dendrites/pathology , Dendrites/ultrastructure , Disease Models, Animal , Endoplasmic Reticulum, Smooth/pathology , Endoplasmic Reticulum, Smooth/ultrastructure , Female , Microscopy, Electron, Transmission , Nerve Degeneration/chemically induced , Nerve Degeneration/pathology , Nerve Degeneration/physiopathology , Purkinje Cells/pathology , Purkinje Cells/ultrastructure , Rats , Rats, Inbred F344 , Sex Characteristics
15.
Alcohol Clin Exp Res ; 30(5): 883-91, 2006 May.
Article in English | MEDLINE | ID: mdl-16634858

ABSTRACT

BACKGROUND: Long-term ethanol consumption in aging rats results in degeneration and regression of the Purkinje neuron (PN) dendritic arbor. One marked ethanol-related change in Purkinje dendrite ultrastructure is dilation of the smooth endoplasmic reticulum (SER) within PN dendritic shafts. The purpose of this study was to determine a time course for ethanol-related dendritic regression in PN dendritic shafts and spines. METHODS: One-hundred eighty aging, male Fischer 344 rats were used. Four durations of treatment (5, 10, 20, and 40 weeks) and 3 dietary treatment groups (60 rats/treatment group) were studied. Ethanol-fed rats received a liquid ethanol diet (35% of dietary calories from ethanol). Pair-fed rats received an isocaloric liquid control diet and chow-fed rats received rat chow and water ad libitum. After each duration of treatment, 45 rats (15/treatment) were euthanized and 2 posterior cerebellar lobules/rat were viewed with electron microscopy and photographed. Diameters of SER profiles within PN shafts and spines were measured with image analysis. RESULTS: Ethanol-related SER dilation in dendritic shafts occurred following 40 weeks of treatment. Ethanol-related SER dilation was not detected in PN dendritic spines. CONCLUSIONS: These results confirm that ethanol-related dilation of SER profiles in PN dendritic shafts occurs following the same duration of treatment as the dendritic regression previously reported in other studies. Degenerating bodies that may be linked to dendritic regression were also identified in PN dendrites.


Subject(s)
Aging , Alcoholism/pathology , Dendrites/ultrastructure , Endoplasmic Reticulum, Smooth/pathology , Purkinje Cells/ultrastructure , Animals , Body Weight , Brain/pathology , Dilatation, Pathologic , Ethanol/administration & dosage , Male , Microscopy, Electron, Transmission , Organ Size , Rats , Rats, Inbred F344
16.
Alcohol ; 35(1): 67-73, 2005 Jan.
Article in English | MEDLINE | ID: mdl-15922139

ABSTRACT

The smooth endoplasmic reticulum (SER), a calcium storage organelle, is essential for normal neuronal function. Dilation of the SER is pathologic and a threat to neuronal calcium homeostasis. Dilation of the SER has been reported within the dendrites of cerebellar Purkinje neurons of aging rats after lengthy ethanol treatment. Ethanol-related alterations of parallel fiber SER have not been investigated despite the fact that such dilation may precede and contribute transsynaptically to SER dilation and degeneration in Purkinje neuron dendrites. Male Fischer 344 rats (n = 120; age = 12 months old) were randomly divided into three dietary groups (40 rats per group) and fed rat chow, the AIN-93M liquid control diet, or the AIN-93M liquid ethanol diet (without water) for 5, 10, 20, or 40 weeks (30 rats per time point). Sections from posterior vermal lobules were viewed with the electron microscope. Maximum and minimum diameters of parallel fiber SER profiles were measured. Ethanol-related dilation of parallel fiber SER was not found after 5, 10, 20, or 40 weeks of treatment. Age-related dilation of parallel fiber SER profiles did occur. These findings support the suggestions that (1) parallel fiber SER, unlike the SER in Purkinje neurons, is insensitive to ethanol and (2) the mechanisms by which ethanol and aging alter cerebellar function and structure are different.


Subject(s)
Aging/pathology , Cerebellum/drug effects , Endoplasmic Reticulum, Smooth/drug effects , Ethanol/toxicity , Animals , Body Weight/drug effects , Cerebellum/ultrastructure , Endoplasmic Reticulum, Smooth/ultrastructure , Male , Organ Size/drug effects , Rats , Rats, Inbred F344
17.
Alcohol Clin Exp Res ; 28(2): 350-64, 2004 Feb.
Article in English | MEDLINE | ID: mdl-15112943

ABSTRACT

This manuscript reviews the proceedings of a symposium organized by Drs. Antonio Noronha and Fulton Crews presented at the 2003 Research Society on Alcoholism meeting. The purpose of the symposium was to examine recent findings on when alcohol induced brain damage occurs, e.g., during intoxication and/or during alcohol withdrawal. Further studies investigate specific brain regions (where) and the mechanisms (why) of alcoholic neurodegeneration. The presentations were (1) Characterization of Synaptic Loss in Cerebella of Mature and Senescent Rats after Lengthy Chronic Ethanol Consumption, (2) Ethanol Withdrawal Both Causes Neurotoxicity and Inhibits Neuronal Recovery Processes in Rat Organotypic Hippocampal Cultures, (3) Binge Drinking-Induced Brain Damage: Genetic and Age Related Effects, (4) Binge Ethanol-Induced Brain Damage: Involvement of Edema, Arachidonic Acid and Tissue Necrosis Factor alpha (TNFalpha), and (5) Cyclic AMP Cascade, Stem Cells and Ethanol. Taken together these studies suggest that alcoholic neurodegeneration occurs through multiple mechanisms and in multiple brain regions both during intoxication and withdrawal.


Subject(s)
Alcohol Drinking/adverse effects , Ethanol/toxicity , Nerve Degeneration/chemically induced , Alcohol Drinking/metabolism , Animals , Cell Differentiation/drug effects , Ethanol/pharmacology , Humans , Nerve Degeneration/metabolism , Nerve Degeneration/pathology , Neurons/cytology , Neurons/drug effects , Neurons/physiology , Societies, Medical , United States
18.
Alcohol Clin Exp Res ; 27(2): 301-9, 2003 Feb.
Article in English | MEDLINE | ID: mdl-12605080

ABSTRACT

This article represents a symposium of the 2002 joint meeting of RSA and ISBRA held in San Francisco. Presentations were Neuropathology of alcohol-related cerebellar damage in humans, by Antony J. Harding; Neuropathological evidence of cerebellar damage in an animal model of alcoholism, by Roberta Pentney and Cynthia Dlugos; Understanding cortical-cerebellar circuits through neuroimaging study of chronic alcoholics, by Peter R. Martin and Mitchell H. Parks; and Functional reorganization of the brain in alcoholism: neuroimaging evidence, by John E. Desmond, S.H. Annabel Chen, Michelle R. Pryor, Eve De Rosa, Adolf Pfefferbaum, and Edith V. Sullivan.


Subject(s)
Alcohol-Induced Disorders, Nervous System/physiopathology , Cerebellar Diseases/physiopathology , Frontal Lobe/physiopathology , Nerve Net/physiopathology , Alcohol-Induced Disorders, Nervous System/diagnosis , Alcohol-Induced Disorders, Nervous System/pathology , Animals , Atrophy , Cerebellar Diseases/diagnosis , Cerebellar Diseases/pathology , Cerebellum/pathology , Dendrites/pathology , Dendrites/physiology , Diagnostic Imaging , Frontal Lobe/pathology , Humans , Nerve Net/pathology , Purkinje Cells/pathology , Purkinje Cells/physiology
19.
Pharmacol Biochem Behav ; 74(2): 471-80, 2003 Jan.
Article in English | MEDLINE | ID: mdl-12479969

ABSTRACT

The effects of acute and chronic ethanol administration on the wild-type (WT), long-fin striped (LFS), and blue long-fin (BLF) strains of zebrafish were investigated. In the LFS strain, acute exposure to 0.25% (v/v) ethanol inhibited the startle reaction and increased both the area occupied by a group of subjects and the average distance between each fish and its nearest neighbor. Similar effects were found in the WT fish although higher concentrations of ethanol were required. No effects on the behavior of the BLF fish were observed with up to 1.0% (v/v) ethanol. Brain alcohol levels were comparable among the three strains precluding a pharmacokinetic explanation for the behavioral results. In LFS zebrafish, behavioral tolerance was observed after 1 week of continual exposure to ethanol. Conversely, chronic ethanol exposure of the WT fish for up to 2 weeks did not result in the development of tolerance, but rather appeared to increase the disruptive action of the drug. The present results suggest the observed strain differences in the effects of ethanol reflect genotypic differences in both the response of the central nervous system (CNS) to ethanol as well as the ability of the CNS to adapt to ethanol exposure. Although preliminary, the present study indicates that the zebrafish is an excellent model system to investigate the genetic determinants involved in regulating the responses to ethanol.


Subject(s)
Behavior, Animal/drug effects , Central Nervous System Depressants/pharmacology , Ethanol/pharmacology , Zebrafish/genetics , Zebrafish/physiology , Animals , Brain/metabolism , Central Nervous System Depressants/metabolism , Dose-Response Relationship, Drug , Ethanol/metabolism , Genotype , Reflex, Startle/drug effects , Species Specificity , Swimming/physiology
20.
Alcohol Clin Exp Res ; 26(11): 1728-33, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12436063

ABSTRACT

BACKGROUND: Ethanol-related synaptic loss, Purkinje neuron dendritic regression, and parallel fiber degeneration have been reported in the molecular layer of the adult cerebellar cortex. The known plasticity of the cerebellar cortex suggests that this region may respond to ethanol-related losses by compensatory remodeling of cerebellar circuitry. Stellate and basket interneurons may play an essential role in the remodeling process. Little is known about ethanol-related effects on cerebellar interneurons or on the GABAergic synapses that they form despite the fact that ethanol-related alterations in these components may contribute to the sensitivity of the cerebellum to ethanol. The paucity of data on GABAergic synapses extends to other synaptic components as well including synaptophysin, a glycoprotein component of synaptic vesicles and a synaptic marker. METHODS: Thirty 12-month-old F344 rats were divided into ethanol-fed, pair-fed, and chow-fed groups (10/group). Ethanol rats were treated for 40 weeks with a liquid diet in which 35% of the calories were derived from ethanol. At the end of treatment, rats were perfused, and tissue processed for quantitative immunohistochemistry of GABA and synaptophysin labels. RESULTS: Levels of GABA within inhibitory synapses formed by stellate and basket neurons and levels of synaptophysin were not altered by long-term ethanol treatment. CONCLUSIONS: Stable levels of GABA within GABAergic basket and stellate interneuron synapses suggest that interneurons in the molecular layer of the cerebellar cortex may not play a major role in remodeling of cerebellar circuitry following long-term ethanol consumption. The lack of ethanol-related alterations in synaptophysin levels reported here suggests that synaptic vesicles may be relatively insensitive to ethanol and that known ethanol-related effects on synapse number are due to other mechanisms.


Subject(s)
Cerebellar Cortex/chemistry , Cerebellar Cortex/drug effects , Ethanol/pharmacology , Synaptophysin/analysis , gamma-Aminobutyric Acid/analysis , Aging/drug effects , Aging/metabolism , Animals , Immunohistochemistry , Male , Rats , Rats, Inbred F344
SELECTION OF CITATIONS
SEARCH DETAIL
...