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2.
Aging Cell ; 21(10): e13713, 2022 10.
Article in English | MEDLINE | ID: mdl-36116133

ABSTRACT

Autophagy is essential for protein quality control and regulation of the functional proteome. Failure of autophagy pathways with age contributes to loss of proteostasis in aged organisms and accelerates the progression of age-related diseases. In this work, we show that activity of endosomal microautophagy (eMI), a selective type of autophagy occurring in late endosomes, declines with age and identify the sub-proteome affected by this loss of function. Proteomics of late endosomes from old mice revealed an aberrant glycation signature for Hsc70, the chaperone responsible for substrate targeting to eMI. Age-related Hsc70 glycation reduces its stability in late endosomes by favoring its organization into high molecular weight protein complexes and promoting its internalization/degradation inside late endosomes. Reduction of eMI with age associates with an increase in protein secretion, as late endosomes can release protein-loaded exosomes upon plasma membrane fusion. Our search for molecular mediators of the eMI/secretion switch identified the exocyst-RalA complex, known for its role in exocytosis, as a novel physiological eMI inhibitor that interacts with Hsc70 and acts directly at the late endosome membrane. This inhibitory function along with the higher exocyst-RalA complex levels detected in late endosomes from old mice could explain, at least in part, reduced eMI activity with age. Interaction of Hsc70 with components of the exocyst-RalA complex places this chaperone in the switch from eMI to secretion. Reduced intracellular degradation in favor of extracellular release of undegraded material with age may be relevant to the spreading of proteotoxicity associated with aging and progression of proteinopathies.


Subject(s)
Microautophagy , Proteome , Aging , Animals , Autophagy/physiology , Endosomes/metabolism , Lysosomes/metabolism , Mice , Protein Transport , Proteome/metabolism
3.
Neuron ; 110(16): 2607-2624.e8, 2022 08 17.
Article in English | MEDLINE | ID: mdl-35767995

ABSTRACT

Regulatory programs governing neuronal death and axon regeneration in neurodegenerative diseases remain poorly understood. In adult mice, optic nerve crush (ONC) injury by severing retinal ganglion cell (RGC) axons results in massive RGC death and regenerative failure. We performed an in vivo CRISPR-Cas9-based genome-wide screen of 1,893 transcription factors (TFs) to seek repressors of RGC survival and axon regeneration following ONC. In parallel, we profiled the epigenetic and transcriptional landscapes of injured RGCs by ATAC-seq and RNA-seq to identify injury-responsive TFs and their targets. These analyses converged on four TFs as critical survival regulators, of which ATF3/CHOP preferentially regulate pathways activated by cytokines and innate immunity and ATF4/C/EBPγ regulate pathways engaged by intrinsic neuronal stressors. Manipulation of these TFs protects RGCs in a glaucoma model. Our results reveal core transcription programs that transform an initial axonal insult into a degenerative process and suggest novel strategies for treating neurodegenerative diseases.


Subject(s)
Optic Nerve Injuries , Retinal Ganglion Cells , Animals , Axons/metabolism , Mice , Mice, Inbred C57BL , Nerve Regeneration/physiology , Optic Nerve Injuries/metabolism , Retinal Ganglion Cells/physiology
4.
Neuron ; 108(5): 876-886.e4, 2020 12 09.
Article in English | MEDLINE | ID: mdl-33108748

ABSTRACT

Myelination facilitates rapid axonal conduction, enabling efficient communication across different parts of the nervous system. Here we examined mechanisms controlling myelination after injury and during axon regeneration in the central nervous system (CNS). Previously, we discovered multiple molecular pathways and strategies that could promote robust axon regrowth after optic nerve injury. However, regenerated axons remain unmyelinated, and the underlying mechanisms are elusive. In this study, we found that, in injured optic nerves, oligodendrocyte precursor cells (OPCs) undergo transient proliferation but fail to differentiate into mature myelination-competent oligodendrocytes, reminiscent of what is observed in human progressive multiple sclerosis. Mechanistically, we showed that OPC-intrinsic GPR17 signaling and sustained activation of microglia inhibit different stages of OPC differentiation. Importantly, co-manipulation of GPR17 and microglia led to extensive myelination of regenerated axons. The regulatory mechanisms of stage-dependent OPC differentiation uncovered here suggest a translatable strategy for efficient de novo myelination after CNS injury.


Subject(s)
Axons/metabolism , Microglia/metabolism , Myelin Sheath/metabolism , Nerve Regeneration/physiology , Nerve Tissue Proteins/blood , Receptors, G-Protein-Coupled/blood , Animals , Axons/ultrastructure , Cell Differentiation/physiology , Cell Proliferation/physiology , Female , Male , Mice , Mice, Transgenic , Microglia/ultrastructure , Myelin Sheath/genetics , Myelin Sheath/ultrastructure , Nerve Fibers, Myelinated/metabolism , Nerve Fibers, Myelinated/ultrastructure , Nerve Tissue Proteins/genetics , Oligodendrocyte Precursor Cells/metabolism , Oligodendrocyte Precursor Cells/ultrastructure , Random Allocation , Receptors, G-Protein-Coupled/genetics
5.
BMC Biol ; 18(1): 8, 2020 01 22.
Article in English | MEDLINE | ID: mdl-31969153

ABSTRACT

BACKGROUND: Two-pore channels (TPCs) release Ca2+ from acidic intracellular stores and are implicated in a number of diseases, but their role in development is unclear. The social amoeba Dictyostelium discoideum proliferates as single cells that aggregate to form a multicellular organism on starvation. Starvation is sensed by the mTORC1 complex which, like TPC proteins, is found on acidic vesicles. Here, we address the role of TPCs in development and under starvation. RESULTS: We report that disruption of the gene encoding the single Dictyostelium TPC protein, TPC2, leads to a delay in early development and prolonged growth in culture with delayed expression of early developmental genes, although a rapid starvation-induced increase in autophagy is still apparent. Ca2+ signals induced by extracellular cAMP are delayed in developing tpc2- cells, and aggregation shows increased sensitivity to weak bases, consistent with reduced acidity of the vesicles. In mammalian cells, the mTORC1 protein kinase has been proposed to suppress TPC channel opening. Here, we show a reciprocal effect as tpc2- cells show an increased level of phosphorylation of an mTORC1 substrate, 4E-BP1. mTORC1 inhibition reverses the prolonged growth and increases the efficiency of aggregation of tpc2- cells. CONCLUSION: TPC2 is required for efficient growth development transition in Dictyostelium and acts through modulation of mTORC1 activity revealing a novel mode of regulation.


Subject(s)
Calcium Channels/genetics , Dictyostelium/physiology , Gene Expression , Mechanistic Target of Rapamycin Complex 1/genetics , Protozoan Proteins/genetics , Autophagy , Calcium Channels/metabolism , Dictyostelium/genetics , Mechanistic Target of Rapamycin Complex 1/metabolism , Protozoan Proteins/metabolism , Signal Transduction
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