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1.
Biofizika ; 35(3): 454-6, 1990.
Article in Russian | MEDLINE | ID: mdl-2169901

ABSTRACT

The investigation of spontaneous hypertension by means of SL-technique is reported. Two component ESR spectra of hypertensive and normotensive rats are simulated. The conventional W/S ratio was shown not to be a measure of relative concentration of two label binding sites ("strongly" and "weakly" immobilized), but it represents alterations in line shapes.


Subject(s)
Computer Simulation , Erythrocyte Membrane/chemistry , Hypertension/blood , Spin Labels , Animals , Electron Spin Resonance Spectroscopy , Maleimides , Rats , Rats, Inbred SHR
2.
Biofizika ; 32(5): 845-58, 1987.
Article in Russian | MEDLINE | ID: mdl-3689828

ABSTRACT

Application of computer analysis to ESR spectra of maleimide spin labels in erythrocyte ghosts and ESR spectra of "spin sacks"--erythrocyte ghosts and liposomes containing concentrated solution of non-penetrating spin label was described. The analysis of the ESR spectra of spin labels gives exhausting information about the parameters of spin hamiltonian, peculiarities of the movement of nitroxyl radicals and their distribution between the cell and medium.


Subject(s)
Computer Simulation , Erythrocyte Membrane/analysis , Spin Labels , Humans , Maleimides
3.
Biofizika ; 31(6): 933-5, 1986.
Article in Russian | MEDLINE | ID: mdl-3026496

ABSTRACT

Computer analysis of ESR spectra recorded under gamma-irradiation of polycrystal and glass-like solutions of nucleotides and nitrous bases was carried out to determine the localization sites of free valency (damage) in DNA macromolecule when interacting with water radiolysis products (OH and H). The analysis was performed on a mini-computer with the program of reconstruction of ESR anisotropic spectra in the variation search regime. When calculating the theoretical spectra anisotropic width of the lines, g-factors and HHS constants on X and Z axes were taken into account. Good reproduction of the experimental spectra on the computer was achieved, as a result the ESR spectra were obtained. Relative contribution of each of OH- and H-adducts of nitrous bases to the total ESR spectrum of gamma-irradiated DNA will be determined in future.


Subject(s)
DNA Damage , DNA/radiation effects , Image Interpretation, Computer-Assisted , Nucleotides/radiation effects , Water , DNA/analysis , Electron Spin Resonance Spectroscopy , Free Radicals , Freezing , Gamma Rays , Nucleotides/analysis , Solutions
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