ABSTRACT
Reaction between ortho-phthalaldehyde and various aroylhydrazines unexpectedly yields N-(1-(2-aryl-hydrazono)isoindolin-2-yl)benzamides as major products along with the predictable 1,2-bis-aroylhydrazones. NMR investigation of the major reaction products indicate the presence of a mixture of geometrical isomers, in various ratios. Single crystal X-ray diffraction confirms the proposed structure and indicates a Z configuration of the CâN double bond substitutents. Optimization of the condensation reaction conditions enabled quantitative isolation of the cyclic isomer. Oxidation of the isomers with bis(trifluoroacetoxy)iodobenzene (PIFA) leads to rapid formation of new highly fluorescent 1,2-bis(5-aryl-1,3,4-oxadiazol-2-yl)benzenes.
Subject(s)
Benzamides/chemistry , Benzamides/chemical synthesis , Hydrazines/chemistry , Iodobenzenes/chemistry , Oxadiazoles/chemistry , Oxadiazoles/chemical synthesis , o-Phthalaldehyde/chemistry , Crystallography, X-Ray , Magnetic Resonance Spectroscopy , Molecular StructureABSTRACT
We report here the synthesis and biochemical properties of a new peptidyl activity-based probe 1 for SUMO proteases, SENPs. The activity-based probe has at its C terminus a glycine-derived fluoromethylketone moiety as a reactive group designed to target the active-site cysteine of SENPs. Based on a study of the interactions between SENPs and SUMOs, we introduced further design elements that allow the activity-based probe to selectively target SENPs at low micromolar to high nanomolar concentrations. Moreover, 1 out-competes SUMO1 from the reversible SUMO1-SENP1 complex, thus suggesting that 1 and SUMO1 share a common binding site on SENP1.
Subject(s)
Endopeptidases/chemistry , Fluorescent Dyes/chemistry , Glycine/chemistry , Ketones/chemistry , Binding Sites , Endopeptidases/biosynthesis , Endopeptidases/metabolism , Enzyme Activation , Fluorescent Dyes/chemical synthesis , Fluorescent Dyes/metabolism , Glycine/analogs & derivatives , Glycine/chemical synthesis , Glycine/metabolism , HEK293 Cells , Humans , Ketones/chemical synthesis , Ketones/metabolism , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/metabolismABSTRACT
Self-assembled inorganic-protein arrays with well-defined and controllable size and structure were obtained through the Fe(II) complexation of protein-conjugated terpyridine units (ligand). The atom-level control of the ligand is obtained through residue-specific conjugation between the complexing unit (terpy) containing an activity-based probe and a corresponding active enzyme (papain). The Fe(II)-based self-assembly performed on this unique building block (ligand) leads to chemical species of unprecedented constitution. The first example presented herein opens the way to a shape and size regime usually reserved to polymers.
Subject(s)
Biotechnology/methods , Ions/chemistry , Metals/chemistry , Papain/metabolism , Protein Array Analysis/methods , Pyridines/chemistry , Electrophoresis, Polyacrylamide Gel , Ferrous Compounds/chemistry , Ions/metabolism , Ligands , Metals/metabolism , Models, Molecular , Molecular Structure , Papain/chemistry , Pyridines/metabolismABSTRACT
Herein we describe the design and synthesis of the first series of di-functional ligands for the directed construction of inorganic-protein frameworks. The synthesized ligands are composed of a metal-ion binding moiety (terpyridine-based) conjugated to an epoxysuccinyl peptide, known to covalently bind active cysteine proteases through the active-site cysteine. We explore and optimize two different conjugation chemistries between the di-functionalized metal-ion ligand and the epoxysuccinyl-containing peptide moiety: peptide-bond formation (with limited success) and Cu(I)-catalysed click chemistry (with good results). Further, the complexation of the synthesized ligands with Fe(II) and Ni(II) ions is investigated: the di-functional ligands are confirmed to behave similarly to the parent terpyridine. As designed, the peptidic moiety does not interfere with the complexation reaction, in spite of the presence of two triazole rings that result from the click reaction. ES-MS together with NMR and UV/Vis studies establish the structure, the stoichiometry of the complexation reactions, as well as the conditions under which chemically sensitive peptide-containing polypyridine ligands can undergo the self-assembly process. These results establish the versatility of our approach and open the way to the synthesis of di-functional ligands containing more elaborated polypyridine ligands as well as affinity labels for different enzyme families. As such, this paper is the first step towards the construction of robust supramolecular species that cover a size-regime and organization level previously unexplored.
