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1.
Biophys Rev ; 15(3): 355-366, 2023 Jun.
Article in English | MEDLINE | ID: mdl-37396453

ABSTRACT

In recent years, transcriptional roadblocking has emerged as a crucial regulatory mechanism in gene expression, whereby other DNA-bound obstacles can block the progression of transcribing RNA polymerase (RNAP), leading to RNAP pausing and ultimately dissociation from the DNA template. In this review, we discuss the mechanisms by which transcriptional roadblocks can impede RNAP progression, as well as how RNAP can overcome these obstacles to continue transcription. We examine different DNA-binding proteins involved in transcriptional roadblocking and their biophysical properties that determine their effectiveness in blocking RNAP progression. The catalytically dead CRISPR-Cas (dCas) protein is used as an example of an engineered programmable roadblock, and the current literature in understanding the polarity of dCas roadblocking is also discussed. Finally, we delve into a stochastic model of transcriptional roadblocking and highlight the importance of transcription factor binding kinetics and its resistance to dislodgement by an elongating RNAP in determining the strength of a roadblock.

2.
Pharmaceuticals (Basel) ; 14(10)2021 Sep 29.
Article in English | MEDLINE | ID: mdl-34681220

ABSTRACT

Engineered phage with properties optimised for the treatment of bacterial infections hold great promise, but require careful characterisation by a number of approaches. Phage-bacteria infection time courses, where populations of bacteriophage and bacteria are mixed and followed over many infection cycles, can be used to deduce properties of phage infection at the individual cell level. Here, we apply this approach to analysis of infection of Escherichia coli by the temperate bacteriophage 186 and explore which properties of the infection process can be reliably inferred. By applying established modelling methods to such data, we extract the frequency at which phage 186 chooses the lysogenic pathway after infection, and show that lysogenisation increases in a graded manner with increased expression of the lysogenic establishment factor CII. The data also suggest that, like phage λ, the rate of lysogeny of phage 186 increases with multiple infections.

3.
ACS Synth Biol ; 10(7): 1633-1639, 2021 07 16.
Article in English | MEDLINE | ID: mdl-34190535

ABSTRACT

We describe a new set of tools for inserting DNA into the bacterial chromosome. The system uses site-specific recombination reactions carried out by bacteriophage integrases to integrate plasmids at up to eight phage attachment sites in E. coli MG1655. The introduction of mutant loxP sites in the integrating plasmids allows repeated removal of antibiotic resistance genes and other plasmid sequences without danger of inducing chromosomal rearrangements. The protocol for Cre-mediated antibiotic resistance gene removal is greatly simplified by introducing the Cre plasmid by phage infection. Finally, we have also developed a set of four independently inducible expression modules with tight control and high dynamic range which can be inserted at specific chromosomal locations.


Subject(s)
Escherichia coli/genetics , Genome, Bacterial , Plasmids/genetics , Recombination, Genetic , Bacteriophages/enzymology , Chromosomes, Bacterial , DNA, Bacterial/genetics , Integrases/genetics
4.
Nucleic Acids Res ; 49(7): e39, 2021 04 19.
Article in English | MEDLINE | ID: mdl-33511418

ABSTRACT

Proteins that can bring together separate DNA sites, either on the same or on different DNA molecules, are critical for a variety of DNA-based processes. However, there are no general and technically simple assays to detect proteins capable of DNA looping in vivo nor to quantitate their in vivo looping efficiency. Here, we develop a quantitative in vivo assay for DNA-looping proteins in Escherichia coli that requires only basic DNA cloning techniques and a LacZ assay. The assay is based on loop assistance, where two binding sites for the candidate looping protein are inserted internally to a pair of operators for the E. coli LacI repressor. DNA looping between the sites shortens the effective distance between the lac operators, increasing LacI looping and strengthening its repression of a lacZ reporter gene. Analysis based on a general model for loop assistance enables quantitation of the strength of looping conferred by the protein and its binding sites. We use this 'loopometer' assay to measure DNA looping for a variety of bacterial and phage proteins.


Subject(s)
Chemistry Techniques, Analytical , DNA, Bacterial/genetics , Escherichia coli Proteins/chemistry , Escherichia coli/genetics , Lac Operon , Lac Repressors/chemistry , Bacteriophage lambda/genetics , Binding Sites , Escherichia coli Proteins/genetics , Lac Repressors/genetics , Nucleic Acid Conformation , Operator Regions, Genetic , Protein Binding , Repressor Proteins/genetics , Viral Regulatory and Accessory Proteins/genetics
5.
Nucleic Acids Res ; 48(21): 12030-12041, 2020 12 02.
Article in English | MEDLINE | ID: mdl-33211866

ABSTRACT

The CII protein of temperate coliphage 186, like the unrelated CII protein of phage λ, is a transcriptional activator that primes expression of the CI immunity repressor and is critical for efficient establishment of lysogeny. 186-CII is also highly unstable, and we show that in vivo degradation is mediated by both FtsH and RseP. We investigated the role of CII instability by constructing a 186 phage encoding a protease resistant CII. The stabilised-CII phage was defective in the lysis-lysogeny decision: choosing lysogeny with close to 100% frequency after infection, and forming prophages that were defective in entering lytic development after UV treatment. While lysogenic CI concentration was unaffected by CII stabilisation, lysogenic transcription and CI expression was elevated after UV. A stochastic model of the 186 network after infection indicated that an unstable CII allowed a rapid increase in CI expression without a large overshoot of the lysogenic level, suggesting that instability enables a decisive commitment to lysogeny with a rapid attainment of sensitivity to prophage induction.


Subject(s)
ATP-Dependent Proteases/genetics , Coliphages/genetics , Endopeptidases/genetics , Escherichia coli Proteins/genetics , Escherichia coli/genetics , Lysogeny , Membrane Proteins/genetics , Prophages/genetics , Viral Proteins/genetics , ATP-Dependent Proteases/metabolism , Coliphages/growth & development , Coliphages/metabolism , Coliphages/radiation effects , Endopeptidases/metabolism , Escherichia coli/metabolism , Escherichia coli/radiation effects , Escherichia coli/virology , Escherichia coli Proteins/metabolism , Membrane Proteins/metabolism , Models, Statistical , Prophages/growth & development , Prophages/metabolism , Prophages/radiation effects , Protein Stability/radiation effects , Proteolysis/radiation effects , Stochastic Processes , Transcriptional Activation , Ultraviolet Rays , Viral Proteins/metabolism
6.
Nucleic Acids Res ; 48(16): 8914-8926, 2020 09 18.
Article in English | MEDLINE | ID: mdl-32789491

ABSTRACT

The Apl protein of bacteriophage 186 functions both as an excisionase and as a transcriptional regulator; binding to the phage attachment site (att), and also between the major early phage promoters (pR-pL). Like other recombination directionality factors (RDFs), Apl binding sites are direct repeats spaced one DNA helix turn apart. Here, we use in vitro binding studies with purified Apl and pR-pL DNA to show that Apl binds to multiple sites with high cooperativity, bends the DNA and spreads from specific binding sites into adjacent non-specific DNA; features that are shared with other RDFs. By analysing Apl's repression of pR and pL, and the effect of operator mutants in vivo with a simple mathematical model, we were able to extract estimates of binding energies for single specific and non-specific sites and for Apl cooperativity, revealing that Apl monomers bind to DNA with low sequence specificity but with strong cooperativity between immediate neighbours. This model fit was then independently validated with in vitro data. The model we employed here is a simple but powerful tool that enabled better understanding of the balance between binding affinity and cooperativity required for RDF function. A modelling approach such as this is broadly applicable to other systems.


Subject(s)
Attachment Sites, Microbiological , DNA Nucleotidyltransferases/metabolism , DNA, Viral/genetics , Lysogeny , Myoviridae/metabolism , Viral Proteins/metabolism , Binding Sites , DNA-Binding Proteins/metabolism , Promoter Regions, Genetic , Protein Binding , Recombination, Genetic
7.
FEBS Lett ; 593(9): 903-917, 2019 05.
Article in English | MEDLINE | ID: mdl-30892685

ABSTRACT

Convergent promoters exert transcriptional interference (TI) by several mechanisms including promoter occlusion, where elongating RNA polymerases (RNAPs) block access to a promoter. Here, we tested whether pausing of RNAPs by obstructive DNA-bound proteins can enhance TI by promoter occlusion. Using the Lac repressor as a 'roadblock' to induce pausing over a target promoter, we found only a small increase in TI, with mathematical modelling suggesting that rapid termination of the stalled RNAP was limiting the occlusion effect. As predicted, the roadblock-enhanced occlusion was significantly increased in the absence of the Mfd terminator protein. Thus, protein roadblocking of RNAP may cause pause-enhanced occlusion throughout genomes, and the removal of stalled RNAP may be needed to minimize unwanted TI.


Subject(s)
DNA-Directed RNA Polymerases/metabolism , Promoter Regions, Genetic/genetics , Transcription, Genetic/genetics , Bacteriophages/genetics , Genes, Reporter/genetics , Models, Biological
8.
Cell Rep ; 26(9): 2419-2433.e3, 2019 02 26.
Article in English | MEDLINE | ID: mdl-30811991

ABSTRACT

Enhancers are ubiquitous and critical gene-regulatory elements. However, quantitative understanding of the role of DNA looping in the regulation of enhancer action and specificity is limited. We used the Escherichia coli NtrC enhancer-σ54 promoter system as an in vivo model, finding that NtrC activation is highly sensitive to the enhancer-promoter (E-P) distance in the 300-6,000 bp range. DNA loops formed by Lac repressor were able to strongly regulate enhancer action either positively or negatively, recapitulating promoter targeting and insulation. A single LacI loop combining targeting and insulation produced a strong shift in specificity for enhancer choice between two σ54 promoters. A combined kinetic-thermodynamic model was used to quantify the effect of DNA-looping interactions on promoter activity and revealed that sensitivity to E-P distance and to control by other loops is itself dependent on enhancer and promoter parameters that may be subject to regulation.


Subject(s)
Enhancer Elements, Genetic , Promoter Regions, Genetic , DNA, Bacterial/chemistry , Escherichia coli Proteins/genetics , Gene Expression Regulation , Lac Repressors/metabolism , Models, Genetic , PII Nitrogen Regulatory Proteins/genetics , Transcription Factors/genetics
9.
Nat Commun ; 8(1): 1628, 2017 11 20.
Article in English | MEDLINE | ID: mdl-29158476

ABSTRACT

DNA looping is a ubiquitous and critical feature of gene regulation. Although DNA looping can be efficiently detected, tools to readily manipulate DNA looping are limited. Here we develop CRISPR-based DNA looping reagents for creation of programmable DNA loops. Cleavage-defective Cas9 proteins of different specificity are linked by heterodimerization or translational fusion to create bivalent complexes able to link two separate DNA regions. After model-directed optimization, the reagents are validated using a quantitative DNA looping assay in E. coli. Looping efficiency is ~15% for a 4.7 kb loop, but is significantly improved by loop multiplexing with additional guides. Bivalent dCas9 complexes are also used to activate endogenous norVW genes by rewiring chromosomal DNA to bring distal enhancer elements to the gene promoters. Such reagents should allow manipulation of DNA looping in a variety of cell types, aiding understanding of endogenous loops and enabling creation of new regulatory connections.


Subject(s)
Bacterial Proteins/chemistry , DNA, Bacterial/genetics , Endonucleases/chemistry , Escherichia coli/genetics , Bacterial Proteins/metabolism , CRISPR-Associated Protein 9 , DNA, Bacterial/chemistry , DNA, Bacterial/metabolism , Endonucleases/metabolism , Enhancer Elements, Genetic , Escherichia coli/chemistry , Escherichia coli/metabolism , Nucleic Acid Conformation , Promoter Regions, Genetic
10.
Nucleic Acids Res ; 45(9): 5074-5085, 2017 May 19.
Article in English | MEDLINE | ID: mdl-28160597

ABSTRACT

Genes are frequently regulated by interactions between proteins that bind to the DNA near the gene and proteins that bind to DNA sites located far away, with the intervening DNA looped out. But it is not understood how efficient looping can occur when the sites are very far apart. We develop a simple theoretical framework that relates looping efficiency to the energetic cost and benefit of looping, allowing prediction of the efficiency of single or multiple nested loops at different distances. Measurements of absolute loop efficiencies for Lac repressor and λ CI using gene expression reporters in Escherichia coli cells show that, as predicted by the model, long-range DNA looping between a pair of sites can be strongly enhanced by the use of nested DNA loops or by the use of additional protein-binding sequences. A combination of these approaches was able to generate efficient DNA looping at a 200 kb distance.


Subject(s)
DNA, Bacterial , Escherichia coli/genetics , Nucleic Acid Conformation , Chromosomes, Bacterial , DNA, Bacterial/chemistry , DNA, Bacterial/metabolism , DNA-Binding Proteins/metabolism , Escherichia coli/chemistry , Escherichia coli/metabolism , Escherichia coli Proteins/metabolism , Lac Repressors/metabolism , Models, Genetic , Protein Binding , Repressor Proteins/metabolism , Viral Proteins/metabolism
11.
Transcription ; 8(2): 120-125, 2017 03 15.
Article in English | MEDLINE | ID: mdl-28129043

ABSTRACT

Transcriptional interference (TI) is increasingly recognized as a widespread mechanism of gene control, particularly given the pervasive nature of transcription, both sense and antisense, across all kingdoms of life. Here, we discuss how transcription factor binding kinetics strongly influence the ability of a transcription factor to relieve or induce TI.


Subject(s)
DNA/metabolism , Transcription Factors/metabolism , Transcription, Genetic , Animals , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , DNA/genetics , DNA-Directed RNA Polymerases/metabolism , Drosophila , Listeria monocytogenes/genetics , Listeria monocytogenes/metabolism , Models, Molecular , Phosphoglycerate Dehydrogenase/genetics , Phosphoglycerate Dehydrogenase/metabolism , Promoter Regions, Genetic , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Saccharomycetales/genetics , Saccharomycetales/metabolism
12.
Nucleic Acids Res ; 44(14): 6625-38, 2016 08 19.
Article in English | MEDLINE | ID: mdl-27378773

ABSTRACT

Transcriptional interference (TI), where transcription from a promoter is inhibited by the activity of other promoters in its vicinity on the same DNA, enables transcription factors to regulate a target promoter indirectly, inducing or relieving TI by controlling the interfering promoter. For convergent promoters, stochastic simulations indicate that relief of TI can be inhibited if the repressor at the interfering promoter has slow binding kinetics, making it either sensitive to frequent dislodgement by elongating RNA polymerases (RNAPs) from the target promoter, or able to be a strong roadblock to these RNAPs. In vivo measurements of relief of TI by CI or Cro repressors in the bacteriophage λ PR-PRE system show strong relief of TI and a lack of dislodgement and roadblocking effects, indicative of rapid CI and Cro binding kinetics. However, repression of the same λ promoter by a catalytically dead CRISPR Cas9 protein gave either compromised or no relief of TI depending on the orientation at which it binds DNA, consistent with dCas9 being a slow kinetics repressor. This analysis shows how the intrinsic properties of a repressor can be evolutionarily tuned to set the magnitude of relief of TI.


Subject(s)
Promoter Regions, Genetic , Repressor Proteins/metabolism , Transcription, Genetic , Viral Regulatory and Accessory Proteins/metabolism , Bacteriophage lambda , CRISPR-Associated Proteins/metabolism , DNA-Directed RNA Polymerases/metabolism , Kinetics , Models, Molecular , Stochastic Processes , Transcription Factors/metabolism
13.
Phys Rev E ; 93(6): 062417, 2016 Jun.
Article in English | MEDLINE | ID: mdl-27415308

ABSTRACT

Methylation of mammalian DNA occurs primarily at CG dinucleotides. These CpG sites are located nonrandomly in the genome, tending to occur within high density clusters of CpGs (islands) or within large regions of low CpG density. Cluster methylation tends to be bimodal, being dominantly unmethylated or mostly methylated. For CpG clusters near promoters, low methylation is associated with transcriptional activity, while high methylation is associated with gene silencing. Alternative CpG methylation states are thought to be stable and heritable, conferring localized epigenetic memory that allows transient signals to create long-lived gene expression states. Positive feedback where methylated CpG sites recruit enzymes that methylate nearby CpGs, can produce heritable bistability but does not easily explain that as clusters increase in size or density they change from being primarily methylated to primarily unmethylated. Here, we show that an interaction between the methylation state of a cluster and its occupancy by nucleosomes provides a mechanism to generate these features and explain genome wide systematics of CpG islands.


Subject(s)
CpG Islands/genetics , Epigenesis, Genetic/physiology , Nucleosomes/metabolism , Animals , DNA Methylation , Promoter Regions, Genetic/genetics
14.
Nucleic Acids Res ; 44(11): 5123-32, 2016 06 20.
Article in English | MEDLINE | ID: mdl-26932361

ABSTRACT

In vertebrates, methylation of cytosine at CpG sequences is implicated in stable and heritable patterns of gene expression. The classical model for inheritance, in which individual CpG sites are independent, provides no explanation for the observed non-random patterns of methylation. We first investigate the exact topology of CpG clustering in the human genome associated to CpG islands. Then, by pooling genomic CpG clusters on the basis of short distances between CpGs within and long distances outside clusters, we show a strong dependence of methylation on the number and density of CpG organization. CpG clusters with fewer, or less densely spaced, CpGs are predominantly hyper-methylated, while larger clusters are predominantly hypo-methylated. Intermediate clusters, however, are either hyper- or hypo-methylated but are rarely found in intermediate methylation states. We develop a model for spatially-dependent collaboration between CpGs, where methylated CpGs recruit methylation enzymes that can act on CpGs over an extended local region, while unmethylated CpGs recruit demethylation enzymes that act more strongly on nearby CpGs. This model can reproduce the effects of CpG clustering on methylation and produces stable and heritable alternative methylation states of CpG clusters, thus providing a coherent model for methylation inheritance and methylation patterning.


Subject(s)
CpG Islands , DNA Methylation , Epigenesis, Genetic , Epigenomics , Algorithms , Cluster Analysis , Computational Biology/methods , Epigenomics/methods , Genome, Human , Humans , Models, Biological
15.
Epigenetics ; 10(4): 293-302, 2015.
Article in English | MEDLINE | ID: mdl-25830651

ABSTRACT

How alternative chromatin-based regulatory states can be made stable and heritable in order to provide robust epigenetic memory is poorly understood. Here, we develop a stochastic model of the silencing system in Saccharomyces cerevisiae that incorporates cooperative binding of the repressive SIR complex and antisilencing histone modifications, in addition to positive feedback in Sir2 recruitment. The model was able to reproduce key features of SIR regulation of an HM locus, including heritable bistability, dependence on the silencer elements, and sensitivity to SIR dosage. We found that antisilencing methylation of H3K79 by Dot1 was not needed to generate these features, but acted to reduce spreading of SIR binding, consistent with its proposed role in containment of silencing. In contrast, cooperative inter-nucleosome interactions mediated by the SIR complex were critical for concentrating SIR binding around the silencers in the absence of barriers, and for providing bistability in SIR binding. SIR-SIR interactions magnify the cooperativity in the Sir2-histone deacetylation positive feedback reaction and complete a double-negative feedback circuit involving antisilencing modifications. Thus, our modeling underscores the potential importance of cooperative interactions between nucleosome-bound complexes both in the SIR system and in other chromatin-based complexes in epigenetic regulation.


Subject(s)
Epigenesis, Genetic , Nucleosomes/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Silencer Elements, Transcriptional , Silent Information Regulator Proteins, Saccharomyces cerevisiae/metabolism , Computer Simulation , Gene Silencing , Histone-Lysine N-Methyltransferase/metabolism , Histones/metabolism , Methylation , Models, Biological , Nuclear Proteins/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Sirtuin 2/metabolism
16.
Proc Natl Acad Sci U S A ; 111(42): E4449-57, 2014 Oct 21.
Article in English | MEDLINE | ID: mdl-25288735

ABSTRACT

Eukaryotic gene regulation involves complex patterns of long-range DNA-looping interactions between enhancers and promoters, but how these specific interactions are achieved is poorly understood. Models that posit other DNA loops--that aid or inhibit enhancer-promoter contact--are difficult to test or quantitate rigorously in eukaryotic cells. Here, we use the well-characterized DNA-looping proteins Lac repressor and phage λ CI to measure interactions between pairs of long DNA loops in E. coli cells in the three possible topological arrangements. We find that side-by-side loops do not affect each other. Nested loops assist each other's formation consistent with their distance-shortening effect. In contrast, alternating loops, where one looping element is placed within the other DNA loop, inhibit each other's formation, thus providing clear support for the loop domain model for insulation. Modeling shows that combining loop assistance and loop interference can provide strong specificity in long-range interactions.


Subject(s)
DNA, Bacterial/chemistry , Escherichia coli/genetics , Binding Sites , DNA, Bacterial/genetics , DNA, Superhelical/chemistry , Gene Expression Regulation, Bacterial , Genes, Reporter , Lac Operon , Lac Repressors , Models, Statistical , Monte Carlo Method , Nucleic Acid Conformation , Operator Regions, Genetic , Promoter Regions, Genetic , Repressor Proteins/chemistry , Stress, Mechanical
17.
J Biol Chem ; 289(46): 32094-32108, 2014 Nov 14.
Article in English | MEDLINE | ID: mdl-25294872

ABSTRACT

The lysogeny promoting protein CII from bacteriophage 186 is a potent transcriptional activator, capable of mediating at least a 400-fold increase in transcription over basal activity. Despite being functionally similar to its counterpart in phage λ, it shows no homology at the level of protein sequence and does not belong to any known family of transcriptional activators. It also has the unusual property of binding DNA half-sites that are separated by 20 base pairs, center to center. Here we investigate the structural and functional properties of CII using a combination of genetics, in vitro assays, and mutational analysis. We find that 186 CII possesses two functional domains, with an independent activation epitope in each. 186 CII owes its potent activity to activation mechanisms that are dependent on both the σ(70) and α C-terminal domain (αCTD) components of RNA polymerase, contacting different functional domains. We also present evidence that like λ CII, 186 CII is proteolytically degraded in vivo, but unlike λ CII, 186 CII proteolysis results in a specific, transcriptionally inactive, degradation product with altered self-association properties.


Subject(s)
Promoter Regions, Genetic , Transcription Factors/genetics , Viral Proteins/genetics , Amino Acid Sequence , Base Sequence , DNA Mutational Analysis , DNA-Directed RNA Polymerases/chemistry , Epitopes/chemistry , Mass Spectrometry , Models, Genetic , Models, Molecular , Molecular Sequence Data , Mutagenesis , Oligonucleotides/chemistry , Plasmids/metabolism , Protein Binding , Protein Structure, Tertiary , Sigma Factor/chemistry , Structure-Activity Relationship , Transcription, Genetic
18.
Nucleic Acids Res ; 42(14): 8861-72, 2014 Aug.
Article in English | MEDLINE | ID: mdl-25034688

ABSTRACT

Genomic DNA is bound by many proteins that could potentially impede elongation of RNA polymerase (RNAP), but the factors determining the magnitude of transcriptional roadblocking in vivo are poorly understood. Through systematic experiments and modeling, we analyse how roadblocking by the lac repressor (LacI) in Escherichia coli cells is controlled by promoter firing rate, the concentration and affinity of the roadblocker protein, the transcription-coupled repair protein Mfd, and promoter-roadblock spacing. Increased readthrough of the roadblock at higher RNAP fluxes requires active dislodgement of LacI by multiple RNAPs. However, this RNAP cooperation effect occurs only for strong promoters because roadblock-paused RNAP is quickly terminated by Mfd. The results are most consistent with a single RNAP also sometimes dislodging LacI, though we cannot exclude the possibility that a single RNAP reads through by waiting for spontaneous LacI dissociation. Reducing the occupancy of the roadblock site by increasing the LacI off-rate (weakening the operator) increased dislodgement strongly, giving a stronger effect on readthrough than decreasing the LacI on-rate (decreasing LacI concentration). Thus, protein binding kinetics can be tuned to maintain site occupation while reducing detrimental roadblocking.


Subject(s)
Transcription, Genetic , Bacterial Proteins/metabolism , DNA, Bacterial/metabolism , DNA-Directed RNA Polymerases/metabolism , Escherichia coli Proteins/metabolism , Lac Repressors/metabolism , Promoter Regions, Genetic , Transcription Factors/metabolism
19.
Nucleic Acids Res ; 42(4): 2235-44, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24288373

ABSTRACT

Inheritance of 5-methyl cytosine modification of CpG (CG/CG) DNA sequences is needed to maintain early developmental decisions in vertebrates. The standard inheritance model treats CpGs as independent, with methylated CpGs maintained by efficient methylation of hemimethylated CpGs produced after DNA replication, and unmethylated CpGs maintained by an absence of de novo methylation. By stochastic simulations of CpG islands over multiple cell cycles and systematic sampling of reaction parameters, we show that the standard model is inconsistent with many experimental observations. In contrast, dynamic collaboration between CpGs can provide strong error-tolerant somatic inheritance of both hypermethylated and hypomethylated states of a cluster of CpGs, reproducing observed stable bimodal methylation patterns. Known recruitment of methylating enzymes by methylated CpGs could provide the necessary collaboration, but we predict that recruitment of demethylating enzymes by unmethylated CpGs strengthens inheritance and allows CpG islands to remain hypomethylated within a sea of hypermethylation.


Subject(s)
CpG Islands , DNA Methylation , Models, Genetic , Inheritance Patterns
20.
Proc Natl Acad Sci U S A ; 111(1): 349-54, 2014 Jan 07.
Article in English | MEDLINE | ID: mdl-24344307

ABSTRACT

Efficient and specific interactions between proteins bound to the same DNA molecule can be dependent on the length of the DNA tether that connects them. Measurement of the strength of this DNA tethering effect has been largely confined to short separations between sites, and it is not clear how it contributes to long-range DNA looping interactions, such as occur over separations of tens to hundreds of kilobase pairs in vivo. Here, gene regulation experiments using the LacI and λ CI repressors, combined with mathematical modeling, were used to quantitate DNA tethering inside Escherichia coli cells over the 250- to 10,000-bp range. Although LacI and CI loop DNA in distinct ways, measurements of the tethering effect were very similar for both proteins. Tethering strength decreased with increasing separation, but even at 5- to 10-kb distances, was able to increase contact probability 10- to 20-fold and drive efficient looping. Tethering in vitro with the Lac repressor was measured for the same 600-to 3,200-bp DNAs using tethered particle motion, a single molecule technique, and was 5- to 45-fold weaker than in vivo over this range. Thus, the enhancement of looping seen previously in vivo at separations below 500 bp extends to large separations, underlining the need to understand how in vivo factors aid DNA looping. Our analysis also suggests how efficient and specific looping could be achieved over very long DNA separations, such as what occurs between enhancers and promoters in eukaryotic cells.


Subject(s)
DNA, Bacterial/genetics , Escherichia coli/genetics , Lac Repressors/genetics , Repressor Proteins/genetics , Viral Regulatory and Accessory Proteins/genetics , Algorithms , DNA, Bacterial/chemistry , Enhancer Elements, Genetic , Escherichia coli Proteins/metabolism , Gene Expression Regulation , Genes, Reporter , Lac Operon , Models, Theoretical , Promoter Regions, Genetic , Protein Interaction Mapping , Thermodynamics , Time Factors
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