[L-Glutamate oxidase from Streptomyces cremeus 510 MGU: effect of nitrogen sources on enzyme secretion]. / L-Glutamatoksidaza iz Streptomyces cremeus 510 MGU: vliianie istochnikov azota na obrazovanie fermenta.

Effect of nitrogen sources (organic complexes and mineral salts) on L-glutamate oxidase synthesis by Streptomyces cremeus 510 MGU was studied. Optimal enzyme production was not provided by any single nitrogen source. The most effective combination of nitrogen sources (soy flour, peptone, ammonium sulfate) was elaborated by the mathematical planning method. The results of experiment allowed to enhance biosynthesis of extracellular L-glutamate oxidase to 2.4-2.6 U/mL. It was shown that L-glutamate oxidase of Streptomyces cremeus 510 MGU is highly specific to substance and is stable during storage of filtrated culture with pH 6.8-9.0.

[Novel L-glutamate oxidase producing organisms: Streptomyces litmocidini and Streptomyces cremeus]. / Novye produtsenty L-glutamatoksidazy-Streptomyces litmocidini i Streptomyces cremeus.

Two novel strains i.e. Streptomyces cremeus 510 and Streptomyces litmocidini 447 producing L-glutamate oxidase were isolated from soil samples and identified. Mathematical design of the experiments made it possible to optimize the fermentation medium composition which provided at least a 5-fold increase of the strain L-glutamate oxidase activity by comparison with the initial activity in the standard medium.