ABSTRACT
Molecular weight related urinary protein analysis is a very valuable diagnostic tool in modern nephrology. However, until recently urine had to be prepared for analysis by several procedures including concentration. Using a new micro-method it was demonstrated that the clinical significance of urinary disc-electrophoresis is much higher than with former systems. Thus, the use of microdisc electrophoresis (Phast system) is recommended for urinary protein analysis in the future.
Subject(s)
Electrophoresis, Disc/methods , Electrophoresis, Polyacrylamide Gel/methods , Proteinuria/diagnosis , Autoanalysis , Humans , Staining and LabelingABSTRACT
Recently a new system for analyzing urinary proteins due to their molecular weight distribution has been introduced. The whole system is automatically driven and uses a silver staining. Due to the small amount of protein necessary for analysis native urine samples can be used. The data presented here show that the automatic micro-disc-electrophoresis (Phast-system) is very useful for any routine testing due to the quick (2 hours) and easily obtainable results with a very high reproducibility. The clinical relevance of urinary protein analysis seems to be much higher using this system.
