Early Bacterial Colonization and Antibiotic Resistance Gene Acquisition in Newborns.

Several studies have recently identified the main factors contributing to the bacterial colonization of newborns and the dynamics of the infant microbiome development. However, most of these studies address large time periods of weeks or months after birth, thereby missing on important aspects of the early microbiome maturation, such as the acquisition of antibiotic resistance determinants during postpartum hospitalization. The pioneer bacterial colonization and the extent of its associated antibiotic resistance gene (ARG) dissemination during this early phase of life are largely unknown. Studies addressing resistant bacteria or ARGs in neonates often focus only on the presence of particular bacteria or genes from a specific group of antibiotics. In the present study, we investigated the gut-, the oral-, and the skin-microbiota of neonates within the first 72 h after birth using 16S rDNA sequencing approaches. In addition, we screened the neonates and their mothers for the presence of 20 different ARGs by directed TaqMan qPCR assays. The taxonomic analysis of the newborn samples revealed an important shift of the microbiota during the first 72 h after birth, showing a clear site-specific colonization pattern in this very early time frame. Moreover, we report a substantial acquisition of ARGs during postpartum hospitalization, with a very high incidence of macrolide resistance determinants and mecA detection across different body sites of the newborns. This study highlights the importance of antibiotic resistance determinant dissemination in neonates during hospitalization, and the need to investigate the implication of the mothers and the hospital environment as potential sources of ARGs.

[Is it Reasonable to Establish an Independently Managed Obstetric Unit in a Small Hospital and Does it Result in Measurable Changes in Quality of Maternity Care?] / Ist eine eigenständige Geburtshilfe in einem Haus der Grund- und Regelversorgung sinnvoll und führt sie zu messbaren Veränderungen in der Versorgung?

INTRODUCTION: An independently managed, specialty obstetric unit in a small hospital setting resulted in measurable changes in quality of maternity care. MATERIAL AND METHODS: We present obstetric data from a level I (basic care) hospital in Frankfurt, Germany. We compare data from the mandatory state register collected in 2013, when the obstetric unit was under single management with the gynaecology department, with data collected in 2016 after the establishment of independent obstetric unit and a specialised service. RESULTS: Between 2013 and 2016, the birth rate in our hospital increased by 46.4%, from 803 to 1176 births/year. CS rates decreased by 8.9%, from 34.9 to 26% (p<0.01). Operative vaginal delivery rates increased by 5.2 (p<0.01%). Transfer of neonates to NICU decreased from 5.6% to 3.1% (p<0.01). Other obstetric interventions also decreased, including induction of labour (10.1 to 9.4%, p=0.632) and rate of episiotomy (13.4 to 1.1%, p<0.01). Rates of severe fetal acidosis (p<0.05) increased from no events in 2013 to 2 events in 2016. There were non-significant reductions in planned caesarean section for primiparous women and repeat caesarean section. CONCLUSION: An independently managed, specialised obstetric unit separate from an Obstetric & Gynaecology Department previously under single management can lead to measurable changes and quality improvement in a short period of time.