ABSTRACT
Preimplantation development is a period of dynamic epigenetic change that begins with remodeling of egg and sperm genomes, and ends with implantation. During this time, parental-specific imprinting marks are maintained to direct appropriate imprinted gene expression. We previously demonstrated that H19 imprinting could be lost during preimplantation development under certain culture conditions. To define the lability of genomic imprints during this dynamic period and to determine whether loss of imprinting continues at later stages of development, imprinted gene expression and methylation were examined after in vitro preimplantation culture. Following culture in Whitten's medium, the normally silent paternal H19 allele was aberrantly expressed and undermethylated. However, only a subset of individual cultured blastocysts (approximately 65%) exhibited biallelic expression, while others maintained imprinted H19 expression. Loss of H19 imprinting persisted in mid-gestation conceptuses. Placental tissues displayed activation of the normally silent allele for H19, Ascl2, Snrpn, Peg3 and Xist while in the embryo proper imprinted expression for the most part was preserved. Loss of imprinted expression was associated with a decrease in methylation at the H19 and Snrpn imprinting control regions. These results indicate that tissues of trophectoderm origin are unable to restore genomic imprints and suggest that mechanisms that safeguard imprinting might be more robust in the embryo than in the placenta.
Subject(s)
Blastocyst/physiology , Genomic Imprinting , Placenta/physiology , RNA, Untranslated/genetics , Alleles , Animals , Autoantigens , Blastocyst/cytology , Cells, Cultured , DNA Methylation , Female , Gestational Age , Kruppel-Like Transcription Factors , Male , Mice , Mice, Inbred C57BL , Placenta/metabolism , Pregnancy , Protein Kinases/genetics , Protein Kinases/metabolism , RNA, Long Noncoding , RNA, Untranslated/metabolism , Ribonucleoproteins, Small Nuclear/genetics , Ribonucleoproteins, Small Nuclear/metabolism , Sex Chromosomes , Transcription Factors/genetics , Transcription Factors/metabolism , snRNP Core ProteinsABSTRACT
DNA methylation of CpG dinucleotides by DNA methyltransferase 1 is implicated in the regulation of transcription and, in particular, the transcription of imprinted genes. Although the oocyte-specific form of Dnmt1 (Dnmt1o) possesses a functional nuclear localization signal, it is predominantly localized in the cytoplasm of the oocyte and preimplantation mouse embryo but undergoes a transient nuclear localization during the eight-cell stage, when the embryos undergo compaction. We report here that Dnmt1o is likely retained in the cytoplasm by an active process, since approximately 70% of DNA methyltransferase activity is retained following permeabilization procedures that result in the release of approximately 75% of oocyte/embryo protein. Treatment of the embryos with agents that disrupt either microfilaments or microtubules has little, if any, effect on the retention of Dnmt1o in permeabilized embryos. While Dnmt1o does not colocalize with either mitochondria or endoplasmic reticulum, it does colocalize with annexin V, which is known to interact with Dnmt1o. We also report that the timing of nuclear entry of Dnmt1o during the eight-cell stage is independent of DNA replication, transcription, and protein synthesis, as well as compaction, cell contact, and cytokinesis. The time of nuclear entry, therefore, appears linked to the time following fertilization, which suggests that a molecular clock governs the time of nuclear import.
