ABSTRACT
Propagated "IN VITRO" shoots and plantlets of ARNICA MONTANA L. (Asteraceae) have been shown to produce sesquiterpene lactones, i.e. helenalin and 11,13-dihydrohelenalin esters. The compounds were detected in green organs only; roots of the plantlets contained no sesquiterpene lactones. The helenalin acetate content in leaves of the plantlets (0.073% dry wt) was 4-times higher than in proliferated shoots (0.016% dry wt). The best rate of shoot multiplication was achieved on MS medium, supplemented with NAA 0.5mg/l and Kn 2.5 mg/l (formation of 22 shoots within 8 weeks).
ABSTRACT
In the present study the tissue culture of a medicinal plant Holarrhena antidisenterica was established. Phytochemical analysis revealed a presence of a few alkaloids in the callus tissues. Two of them were determined by TLC as conessine and conimine. A screening of antibacterial activity of alkaloid extract was performed.
