1.
J Clin Lab Anal
; 16(6): 295-8, 2002.
Article
in English
| MEDLINE
| ID: mdl-12424802
ABSTRACT
An enzyme-linked immunoassay (ELISA) using purified 5/B Echinococcus enriched antigen was used to follow IgG, IgM, and IgA antibody levels pre- and posttreatment or surgical removal of hydatid cysts. The sensitivity was 97%, 37.5%, and 54.5%, respectively, and the specificity was 95.7%, 100%, and 98.9%, respectively. All isotypes could be detected 3 years after surgical removal of cysts in patients showing no remaining cyst evidence. This was especially true for IgG, which persisted in 85.2% of the patients. The data indicate that antigen purification improves specificity without affecting sensitivity, although this new antigen offers no advantages in the postsurgical monitoring of the patients.
Subject(s)
Echinococcosis/immunology , Enzyme-Linked Immunosorbent Assay/methods , Immunoglobulin A/analysis , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Adolescent , Adult , Aged , Aged, 80 and over , Antibody Specificity , Antigen-Antibody Complex , Antigens, Helminth/immunology , Echinococcosis/blood , Echinococcosis/surgery , Female , Humans , Immunoglobulin A/immunology , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Isoantigens/metabolism , Male , Middle Aged , Postoperative Period
2.
Folia Parasitol (Praha)
; 49(2): 163-4, 2002.
Article
in English
| MEDLINE
| ID: mdl-12194490
Subject(s)
Cryptosporidiosis/transmission , Cryptosporidium parvum/genetics , Cryptosporidium parvum/isolation & purification , Houseflies/microbiology , Insect Vectors/microbiology , Animals , Cattle , Cryptosporidiosis/epidemiology , DNA Primers/analysis , Feces/microbiology , Humans , Oocysts/isolation & purification , Polymerase Chain Reaction/methods , Prevalence , RNA, Ribosomal, 18S/analysis
3.
Enferm. infecc. microbiol. clín. (Ed. impr.)
; 19(1): 29-30, ene. 2001.
Article
in Es
| IBECS
| ID: ibc-2736
ABSTRACT
No disponible
