ABSTRACT
We have used modified standard methods and derived new formulae to quantitate cell permeability (P), cell/media partitioning (λ), and intracellular sequestration or binding rate constants (m) for cultured S49 murine lymphoma cells in suspension. Using 15 standard compounds and anticancer drugs, we found quantitative relationships among log P, log PO (octanol/pH 7.4 buffer partition coefficient), and molecular weight (MW) such that logP = -4.5 + 0.56log (PO(MW)(-1/2)). A good correlation among P, λ, and MW was also determined with λ = 0.67 + 5890 gm(1/2) cm(-1) sec (P (MW)(1/2)). These studies show that there is a strong partitioning (λ) dependence to molecular weight and permeability that can be predicted even for known carrier-transported and biotransformable compounds. Furthermore, results of this study show that the slope of the plot of permeability and lipophilicity is not necessarily unity as has been postulated from the results of other studies.
ABSTRACT
Clinical and pathologic features were assessed in a group of 48 patients with Hodgkin's disease who developed recurrences in treated sites. The histopathologic findings in the initial diagnostic lymph node biopsies were compared with those in the recurrences. Recurrences showed a more pleomorphic appearance and were more difficult to subclassify than the initial diagnostic biopsies. In addition, no histologic features were found in the recurrences that could identify those patients who later died of Hodgkin's disease versus those those who were successfully re-treated.
