Research of biological properties of enterovirus strains associated with ischemic stroke.

OBJECTIVE: Introduction: The research of biological properties of enteroviruses associated with ischemic stroke (IS) allows us to identify their intratypic differences. The aim: to identify genetic markers of strains of enteroviruses associated with IS. PATIENTS AND METHODS: Materials and methods: 11 strains of enteroviruses isolated from the serum of patients with IS were identified in the virus neutralization test. Genetic markers of isolated strains (Abent, marker S, marker rct40) were determined. RESULTS: Results: Eleven strains of enteroviruses were isolated from the serum of patients with IS. Eight viruses: Coxsackie B viruses (serotypes 2, 3, 4) and ECHO viruses (serotypes 6, 9, 27 (two strains), 29) were identified in these strains. Other three strains of enteroviruses were unidentified. Different combinations of genetic markers were found. Seven strains of enteroviruses (Coxsackie B2, B3, ECHO 6, ECHO 9, ECHO 27 (two strains) and one unidentified virus) had virulence markers: Abent-, rct40+ and S-. Three strains (Coxsackie B4, ECHO 29, one unidentified virus) had markers: Abent-, rct40+, S+. Another one unidentified virus had markers: Abent+, rct40+, S -. CONCLUSION: Conclusions: All 11 isolates of enteroviruses associated with IS had rct40+ marker, 10 of the 11 isolates had marker Abent- and 8 of 11 isolates had marker S-. The research of genetic markers allows to perform typic and intratypic differentiation of strains of enteroviruses associated with the IS.

The role of the persistent enterovirus infection in development of acute stroke.

INTRODUCTION: The role of enteroviruses in development of dilated cardiomyopathy, myocardial infarction, myocarditis, pericarditis is known. THE AIM: To examine the role of chronic enterovirus infections in development of acute stroke. MATERIAL AND METHODS: Blood samples from 72 patients with acute stroke (study group) and 35 patients without vascular disease (control group) were investigated by reverse transcription polymerase chain reaction for the presence of enterovirus RNA, by using virological method to detect enteroviruses, by ELISA for the levels of IgM and IgG antibodies to enteroviruses. RESULTS: The enteroviruses genomes were detected significantly often in the serum of patients with stroke (23,6 ± 5,9%) than in control group (2,9 ± 2,8%). The viruses were isolated and were identified as Coxsackie B (serotypes 2, 3, 4) and ECHO (serotypes 6, 9, 27 (two strains), 29), three strains have not been identified in study group. IgM to enteroviruses were not found in the sera of both groups of patients. IgG to enteroviruses were detected in 17 patients in study group (23,6 ± 5,9%) and 2 patients in control group (5,7 ± 3,9%). The presence of enteroviruses genomes and IgG in sera of patients in control group (11,1 ± 3,7%) indicate the persistence of enteroviruses. The proportion of patients with IgG to enteroviruses in sera is higher in study group (12,5 ± 3,9%) than in control group (5,7 ± 3,9%). CONCLUSION: The enterovirus infections play trigger role in development of acute stroke.