Fungal Pathogens Associated With Canker Diseases of Almond in California.

Almond canker diseases are destructive and can reduce the yield as well as the lifespan of almond orchards. These diseases may affect the trunk and branches of both young and mature trees and can result in tree death soon after orchard establishment in severe cases. Between 2015 and 2018, 70 almond orchards were visited throughout the Central Valley of California upon requests from farm advisors for canker disease diagnosis. Two major canker diseases were identified, including Botryosphaeriaceae cankers and Ceratocystis canker. In addition, five less prevalent canker diseases were identified, including Cytospora, Eutypa, Diaporthe, Collophorina, and Pallidophorina canker. Seventy-four fungal isolates were selected for multilocus phylogenetic analyses of internal transcribed spacer region ITS1-5.8S-ITS2 and part of the translation elongation factor 1-α, ß-tubulin, and glyceraldehyde 3-phosphate dehydrogenase gene sequences; 27 species were identified, including 12 Botryosphaeriaceae species, Ceratocystis destructans, five Cytospora species, Collophorina hispanica, four Diaporthe species, two Diatrype species, Eutypa lata, and Pallidophorina paarla. The most frequently isolated species were Ceratocystis destructans, Neoscytalidium dimidiatum, and Cytospora californica. Pathogenicity experiments on almond cultivar Nonpareil revealed that Neofusicoccum parvum, Neofusicoccum arbuti, and Neofusicoccum mediterraneum were the most virulent. Botryosphaeriaceae cankers were predominantly found in young orchards and symptoms were most prevalent on the trunks of trees. Ceratocystis canker was most commonly found in mature orchards and associated with symptoms found on trunks or large scaffold branches. This study provides a thorough examination of the diversity and pathogenicity of fungal pathogens associated with branch and trunk cankers of almond in California.

Characterization of Fusarium and Neocosmospora Species Associated With Crown Rot and Stem Canker of Pistachio Rootstocks in California.

California produces 99.1% of pistachios grown in the United States, and diseases affecting pistachio rootstocks represent a constant challenge to the industry. Field surveys of fungi associated with pistachio rootstocks with symptoms of crown rot and stem canker in three central California counties followed by phylogenetic analyses of translation elongation factor 1-α and second largest subunit of RNA polymerase II gene fragments identified three Fusarium species (Fusarium equiseti, Fusarium oxysporum, and Fusarium proliferatum) and two Neocosmospora species (Neocosmospora falciformis and Neocosmospora solani). F. oxysporum and N. falciformis were the fungal species most frequently recovered from symptomatic pistachio trees. Inoculations of detached twigs of cultivar Kerman pistachio Pioneer Gold I and clonal University of California, Berkeley I (UCBI) rootstocks showed that all five species could colonize pistachio wood and cause vascular discolorations. Pathogenicity tests in potted pistachio trees completed Koch's postulates and confirmed that F. oxysporum, F. proliferatum, N. falciformis, and N. solani were capable of producing rot and discoloration in stems of clonal UCBI rootstocks, the most widely planted pistachio rootstock in California. To our knowledge, this study is the first to present insights into the biodiversity and biology of Fusarium and Neocosmospora species associated with pistachio trees in California.

First Report of the Peach Root-Knot Nematode, Meloidogyne floridensis Infecting Almond on Root-Knot Nematode Resistant 'Hansen 536' and 'Bright's Hybrid 5' Rootstocks in California, USA.

In April-August 2018, samples of galled roots with rhizosphere soil were collected from almond orchards in Atwater, Merced County and Bakersfield, Kern County, California. Almond trees (Prunus dulcis) grafted on 'Hansen 536' and 'Brights Hybrid®5' (peach-almond hybrid) rootstocks showed strong symptoms of growth decline. Extracted root-knot nematodes were identified by both morphological and molecular methods as M. floridensis. Meloidogyne floridensis was initially found in Florida, USA, and has not been reported from any other states and countries. This is a first report of M. floridensis in California and outside of Florida.In April-August 2018, samples of galled roots with rhizosphere soil were collected from almond orchards in Atwater, Merced County and Bakersfield, Kern County, California. Almond trees (Prunus dulcis) grafted on 'Hansen 536' and 'Brights Hybrid®5' (peach-almond hybrid) rootstocks showed strong symptoms of growth decline. Extracted root-knot nematodes were identified by both morphological and molecular methods as M. floridensis. Meloidogyne floridensis was initially found in Florida, USA, and has not been reported from any other states and countries. This is a first report of M. floridensis in California and outside of Florida.

Deep injection and the potential of biochar to reduce fumigant emissions and effects on nematode control.

Reducing fumigant emissions is essential for minimizing the environmental impacts of pre-plant soil fumigation. Low permeability plastic films are effective at reducing emissions but have high initial purchase, installation, and disposal costs. The objective of this study was to evaluate if deep fumigant injection and biochar soil amendments can reduce emissions, improve fumigant distribution in soil, and provide acceptable control of plant parasitic nematodes. A pre-plant soil fumigation trial was conducted in a commercial orchard in the San Joaquin Valley, CA, USA. Treatments included two rates of Telone® C-35 (a mixture of 1,3-dichloropropene and chloropicrin) under totally impermeable film or with no surface seal, two injection depths (45 or 65 cm), and two biochar rates (20 or 40 ton ha-1). Emission rates were generally low due to rain events encountered during the trial, but data clearly showed that the deep injection enhanced fumigant delivery to depths below 60 cm and resulted in significantly lower peak emission compared to the standard injection depth. Biochar applied at 40 ton ha-1 had the lowest emission rates during 1-month monitoring period. Although variability in nematode survival was high, tarped, deep injection, and biochar treatment showed lower survival of nematodes at various depths. Increase in fumigant persistence, especially chloropicrin, was observed in this study, likely due to the high soil moisture and low temperature. All data indicate that biochar amendments can help reduce fumigant emissions without reducing nematode control; however, additional research is needed to optimize treatments, determine the affordability of various biochar materials, and validate results under a range of field conditions.

Climatic Constraints to the Potential of Microsphaeropsis amaranthi as a Bioherbicide for Common Waterhemp.

ABSTRACT The fungal plant pathogen Microsphaeropsis amaranthi is a candidate bioherbicide for the control of weedy Amaranthus species since it grows and sporulates readily in culture, is a pathogen of a number of important weed species, and is host-restricted to the family Amaranthaceae. This study was designed to determine the optimum and limiting environmental conditions for the efficacy of foliar applications of M. amaranthi for the control of common waterhemp. The greatest disease severity and the greatest plant biomass reductions were found when a prolonged leaf wetness period of >/= 12 h at 18 to 24 degrees C was provided after application. Disease severity and plant biomass reductions were greater when plants were treated at an earlier stage of development. Disease severity and impact were limited when shorter leaf wetness periods were provided, with negligible disease observed on plants provided with