ABSTRACT
The coexistence of interleukin (IL)-1beta with IL-1 receptor antagonist (ra) in bovine colostrum and the possibility of simultaneous transfer of these cytokines to neonates via colostrum have been demonstrated. In the present study, we investigated the effect of IL-1ra on the mitogenic response of calf peripheral blood mononuclear cells (PBMC) stimulated by concanavalin A (ConA), which was mediated by IL-1. Pretreatment of PBMC with recombinant bovine (rb) IL-1ra alone significantly suppressed the proliferation of ConA-stimulated cells. However, in the presence of rbIL-1beta, the suppressive activity of rbIL-1ra was counteracted. These results suggest that coexistence of IL-1ra with IL-1 in colostrum may have no effect on the activation of the neonatal immune system by IL-1beta.
Subject(s)
Cattle/immunology , Concanavalin A/immunology , Interleukin-1/immunology , Leukocytes, Mononuclear/immunology , Sialoglycoproteins/immunology , Animals , Animals, Newborn , Cell Division/drug effects , Colostrum/physiology , Concanavalin A/pharmacology , Dose-Response Relationship, Immunologic , Drug Interactions , Female , Interleukin 1 Receptor Antagonist Protein , Interleukin-1/antagonists & inhibitors , Leukocytes, Mononuclear/drug effects , Recombinant Proteins/immunology , Recombinant Proteins/pharmacology , Sialoglycoproteins/pharmacologyABSTRACT
Hepatic lesions in 25 sika deer (Cervus nippon Temminck) aged 1-15 days, affected by selenium-deficiency cardiomyopathy, were examined histopathologically. Characteristic pathological findings, induced by stagnation of the plasma proteins of the cytoplasm, consisted of vacuolar degeneration of hepatocytes, formation of hyalin droplets, and ceroid-lipofuscinosis. Electron microscopically, these changes were closely associated with degeneration of the endoplasmic reticulum and mitochondria. Peroxisomes, which were observed around the vacuoles, were regarded as a reactive result of membrane disturbance caused by a decrease in glutathione peroxidase (GSH-Px)