ABSTRACT
No disponible
Subject(s)
Humans , Female , Aged , Intercellular Signaling Peptides and Proteins/metabolism , Amyloidosis/diagnosis , Amyloidosis/metabolism , Gas Chromatography-Mass Spectrometry , GenotypeABSTRACT
Mannan and carboxymethylcellulose, previously activated by periodate oxidation, were grafted with mono-6-butylenediamino-6-deoxy-beta-cyclodextrin derivatives by reductive alkylation in the presence of sodium borohydride. The formation of supramolecular complexes between these polymers and Naproxen was confirmed by fluorescence spectroscopy. The solubility of the drug was 3.8-4.6 fold increased in the presence of the cyclodextrin-grafted polysaccharides. The in vivo anti-inflammatory property of Naproxen was 1.7 times higher after supramolecular association with beta-cyclodextrin-branched mannan.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/chemistry , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Carboxymethylcellulose Sodium/chemistry , Cyclodextrins/chemistry , Mannans/chemistry , Naproxen/chemistry , Naproxen/pharmacology , Animals , Anti-Inflammatory Agents, Non-Steroidal/chemical synthesis , Carrageenan/metabolism , Fluorescence , Magnetic Resonance Spectroscopy , Male , Naproxen/chemical synthesis , Rats , Rats, Wistar , SolubilityABSTRACT
Mannan from Sacharomyces cerevisiae was activated by oxidation with NaIO4 (sodium m-periodate) and further linked to SOD (superoxide dismutase) via reductive alkylation with NaBH4 (sodium borohydride). The gly-cosidated enzyme contained an average of 1,2 mol of polysaccharide per mol of protein and retained 52 per cent of its initial activity. The modified enzyme was 560-fold more resistant to inactivation with H2O2 and acquired a lectin-recognition capacity in respect of concanavalin A. The anti-inflammatory activity of SOD was increased 2-fold and its plasma half-life time was prolonged from 4.8 min to 1.7 h after glycosylation with the polymer(AU)
Subject(s)
Animals , Male , Mice , Mannans/pharmacology , Superoxide Dismutase/pharmacology , Superoxide Dismutase/therapeutic use , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/therapeutic useABSTRACT
Ventilator-associated pneumonia (VAP) is one of the most important infectious diseases in the intensive care unit (ICU). In some series the attributable mortality of VAP may reach 30%, and the adequacy of the initial empirical treatment greatly influences the prognosis. Treatment failure can be anticipated in approximately 30 to 40% of patients developing pneumonia during mechanical ventilation and this situation is associated with an adverse outcome in terms of morbidity. For these reasons, the lack of response to empirical antibiotic treatment should be recognized early in the course of VAP, and a clear strategy for clarifying the causes of failure should be implemented. Resolution of VAP depends not only on the accuracy of microbial diagnosis but also on comorbidities, the response of the host to the infection, concomitant infections, and the correct clinical diagnosis. Treatment failure can be defined by the pattern of resolution of VAP, which is clearly established in the literature.
Subject(s)
Anti-Bacterial Agents/therapeutic use , Pneumonia, Bacterial/drug therapy , Ventilators, Mechanical/adverse effects , Cross Infection/complications , Cross Infection/drug therapy , Drug Resistance, Bacterial , Humans , Pneumonia, Bacterial/complications , Pneumonia, Bacterial/etiology , Treatment FailureABSTRACT
Mannan from Sacharomyces cerevisiae was activated by oxidation with NaIO(4) (sodium m-periodate) and further linked to SOD (superoxide dismutase) via reductive alkylation with NaBH(4) (sodium borohydride). The glycosidated enzyme contained an average of 1.2 mol of polysaccharide per mol of protein and retained 52% of its initial activity. The modified enzyme was 560-fold more resistant to inactivation with H(2)O(2) and acquired a lectin-recognition capacity in respect of concanavalin A. The anti-inflammatory activity of SOD was increased 2-fold and its plasma half-life time was prolonged from 4.8 min to 1.7 h after glycosylation with the polymer.
Subject(s)
Inflammation/drug therapy , Mannans/chemistry , Superoxide Dismutase/chemistry , Superoxide Dismutase/therapeutic use , Alkylation , Animals , Borohydrides/chemistry , Carbohydrate Conformation , Carbohydrate Sequence , Carrageenan , Enzyme Activation/drug effects , Glycosylation , Hydrogen Peroxide/pharmacology , Inflammation/chemically induced , Male , Molecular Sequence Data , Oxidation-Reduction , Periodic Acid/chemistry , Rats , Rats, Wistar , Saccharomyces cerevisiae/chemistry , Structure-Activity Relationship , Superoxide Dismutase/blood , Time FactorsABSTRACT
O-carboxymethylchitin (molecular weight = 1.07 x 10(5), degree of carboxymethylation = 80%, degree of N-acetylation = 91%) was chemically attached to superoxide dismutase by the formation of amide linkages through a carbodiimide catalyzed reaction. The glycosidated enzyme contained about 1.8 mole of polysaccharide per mole of protein and retained 57% of the initial catalytic activity. The anti-inflammatory activity of the enzyme was 2.4 times increased after conjugation with the polysaccharide. The modified superoxide dismutase preparation was remarkably more resistant to inactivation with H(2)O(2) and its plasma half-life time was prolonged from 4.8 min to 69 h.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/pharmacokinetics , Chitin/analogs & derivatives , Chitin/metabolism , Superoxide Dismutase/pharmacology , Superoxide Dismutase/pharmacokinetics , Animals , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/metabolism , Edema/drug therapy , Extremities , Glycosylation , Rats , Superoxide Dismutase/chemistry , Superoxide Dismutase/metabolismABSTRACT
Se considera el arsénico como uno de los elementos extremadamente tóxicos para el hombre, y en los últimos años ha sido informado por la Agencia Internacional del Cáncer como cancerígeno para el organismo humano. En este trabajo se presentan los residuales de arsénico en 192 muestras de especies marinas capturadas en zonas costeras de las provincias de Pinar del Río y Matanzas. Para la determinación de este metal se empleó un método espectrofotométrico de absorción atómica con generación de arsina. Todos los resultados encontrados se comportaron por debajo del límite de tolerancia que se recomienda por la norma CAME de 1982 (AU)
Subject(s)
Arsenic Poisoning , Fish Products/analysis , CubaABSTRACT
Se considera el arsénico como uno de los elementos extremadamente tóxicos para el hombre, y en los últimos años ha sido informado por la Agencia Internacional del Cáncer como cancerígeno para el organismo humano. En este trabajo se presentan los residuales de arsénico en 192 muestras de especies marinas capturadas en zonas costeras de las provincias de Pinar del Río y Matanzas. Para la determinación de este metal se empleó un método espectrofotométrico de absorción atómica con generación de arsina. Todos los resultados encontrados se comportaron por debajo del límite de tolerancia que se recomienda por la norma CAME de 1982
