ABSTRACT
A rapid and highly sensitive method for detecting hog cholera virus (HCV) based on a reverse transcription of the polymerase chain reaction (RT-PCR) is developed. Primers complementary to the most homologous sites of virus genome in an area coding the precursor for glycoproteins gp44/gp48 are selected. Detection of the virus in pathological material by the RT-PCR showed that use of these primers in amplification allows detection of different HCV strains.
Subject(s)
Classical Swine Fever Virus/isolation & purification , Animals , Base Sequence , Cattle , Cell Line , Classical Swine Fever Virus/genetics , DNA, Complementary , DNA, Viral , Molecular Sequence Data , Polymerase Chain Reaction , Sensitivity and Specificity , Sequence Homology, Nucleic Acid , Species SpecificityABSTRACT
The history and modern status of tuberculosis are analyzed from epidemiological viewpoint. Different repeating genetic elements of mycobacterial genome are described, which have been recently used to develop methods for typing of pathogenic mycobacterial strains. Genome fingerprinting is compared with the traditional methods of epidemiology. The prevalence of Mycobacterium bovis in domestic and wild animals is analyzed.
Subject(s)
DNA Fingerprinting , Mycobacterium tuberculosis/genetics , Tuberculosis/genetics , Animals , Cattle , Humans , Mycobacterium bovis/classification , Mycobacterium bovis/genetics , Mycobacterium tuberculosis/classification , Serotyping , Tuberculosis/epidemiology , Tuberculosis/transmission , Tuberculosis, Bovine/epidemiology , Tuberculosis, Bovine/genetics , Tuberculosis, Bovine/transmissionABSTRACT
Analysis is given of the basis of epidemiological parameters in two districts of the Novosibirsk region, depending on the duration of the unfavourable epizootic situation. Subjects with extensive residual changes were registered 10 times as often in the district with the unfavourable epizootic situation as in the district with fresh tuberculosis outbreak in animals. Studies of infectivity among children and adolescents at the height of tuberculosis outbreak in animals showed that reversion was established in 8.6%, while the share of children with hyperergic reactions increased by 4 times and exceeded the average regional index by 8 times. Children and adolescents from the district with the unfavourable situation for tuberculosis had a low level of reversion (0.3%), but the level of infectivity in children of the decreed ages was high. The complex of health-improvement, prophylactic and veterinary measures has been suggested.
