ABSTRACT
We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling. Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed. The sensing ability of the AuNPs with biopolymers was investigated via various analytical techniques. The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods. AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468. Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar. This method was found to be easy and safest in comparison with the universally accepted cyanidation process. In the future, small gold jewelry makers and related industries would benefit from the proposed gold-recycling process and it might contribute to their socio-economic growth. The methodologies proposed are also beneficial for trace-level forensic investigation.
Subject(s)
COVID-19 , Metal Nanoparticles , alpha-Cyclodextrins , COVID-19/diagnosis , COVID-19 Testing , Coloring Agents , Cytotoxins , DNA , Gold/chemistry , Humans , Male , Metal Nanoparticles/chemistry , Semen , Serum Albumin, Bovine/chemistry , SugarsABSTRACT
We developed a cost-effective and eco-friendly click biosynthesis of small molecule quercetin-gold quantum dots (QRT-AuQDs) involving quick conjugation using an ultrasonication method at ambient temperature by utilizing QRT and gold ions in the proportion of 0.1 : 1 (molar ratio). A comparatively very short amount of time (60 seconds) was required as compared to conventional procedures. The present biomimetics research relates to the isolation of bioactive QRT by the circularly spread silica gel layer technique (CSSGLT) and characterization (UV-Vis, FTIR, NMR and DSC analysis). Characterization of the synthesized QRT-AuQDs conjugated complex was carried out by UV-Vis, HR-TEM, DLS, zeta potential and X-ray diffraction. The main objective of the present work was to study the comparative anticancer activity of QRT and QRT-AuQDs on human lung cancer HOP-62 and leukemia K-562 cell lines. The results suggested that QRT-AuQDs showed potential for applications in anticancer treatment and were found to be a more cytotoxic agent in comparison to QRT, causing > 50% inhibition of cancer cells at the concentration < 10-7 M. Hence, small molecule conjugated QRT-AuQDs can be used as a promising material for biomedical, bioengineering and anti-infectives applications.
ABSTRACT
The derivatives of 3-hydroxyimino-5-methyl-2-hexanone oxime have been obtained in good yield by its reactions either with hydrazine hydrate or phenyl hydrazine, respectively. IR and 1H NMR spectral data of these compounds have been discussed. All the newly synthesised compounds have been tested for their biological activity against S. auerus, S. typhi, C. albicans, A. niger, S. cerevisiae and M. tuberculosis H47RV.
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Hexanones/chemical synthesis , Hydrazones/chemical synthesis , Oximes/chemical synthesis , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Hexanones/chemistry , Hexanones/pharmacology , Hydrazones/chemistry , Hydrazones/pharmacology , Microbial Sensitivity Tests/methods , Microbial Sensitivity Tests/statistics & numerical data , Oximes/chemistry , Oximes/pharmacologyABSTRACT
A new oxime, 3-hydroxyimino-5-methyl-2-hexanone (HIMH) has been synthesized by the reaction of 1-pentyl nitrite with 5-methyl-2-hexanone under acidic conditions. The subsequent treatment of HIMH with NH2OH x HCl gives 5-methyl-2,3-hexanedione dioxime (H2MHDDO). The structures of these compounds have been confirmed by physicochemical and spectral data. A preliminary screening of these compounds for biological activity against several microorganisms has indicated that they are selective growth inhibitors of m-tuberculosis, in particular.
