ABSTRACT
This present study was designed to investigate the production of huperzine A (HupA), an acetylcholine inhibitor, which was produced by an endophytic fungi isolated from Huperzia serrata. Screening of 94 endophytic fungal isolates obtained from plant H. serrata was carried out for the production of HupA. Their morphological characteristics were studied and rDNA sequence analysis was carried out. The cultures were grown in liquid culture medium and the extracted metabolites were analyzed by thin layer chromatography and high performance liquid chromatograph for the presence of HupA. The DPPH scavenging ratio and inhibition ratio of acetylcholinesterase (AchE) of the same were determined. 3 out of 94 strains i.e. S29, L44 and S94 showed significant AchE-inhibitory activity and antioxidant activity. Strain L44 which exhibited maximum yield of HupA (37.63 µg/g on dry weight basis) was identified as Trichoderma species by ITS sequence analysis. In conclusion, endophytic fungi from H. serrata can be used as a new resource of HupA.
Subject(s)
Alkaloids/metabolism , Endophytes/classification , Endophytes/metabolism , Huperzia/microbiology , Sesquiterpenes/metabolism , Trichoderma/classification , Trichoderma/metabolism , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Cluster Analysis , DNA, Fungal/chemistry , DNA, Fungal/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Endophytes/isolation & purification , Molecular Sequence Data , Phylogeny , Sequence Analysis, DNA , Trichoderma/isolation & purificationABSTRACT
There are many hydrogen bonds in coal, which affect the chemical structure and properties of coal. FTIR has been applied to the characterization study of the hydrogen bonds of Dongpang coals, which were under drop weight impact. There exists five kinds of hydrogen bonds in the coal: free OH groups, OH...π, OH...OH, cyclic OH tetramers and OH...N. Absorption strength of intermolecular hydrogen bonds markedly declined after impact. Free OH groups mechanical-power chemical reacted in drop weight impact testing. The infrared spectrum were curve-resolved into their component bands. The absorption strength of various hydrogen bonds decreased with the increase of impact energy, but the trend was slowing. By statistical relationship between then, we find then complying with power function relationship. By comparing the exponents of fitted equations, we concluded that failure sensitivity sequence of hydrogen bonds to the impact: free OH groups > cyclic OH tetramers > OH...N > OH...π > OH...OH.
