Research on Emotion Recognition Method of Cerebral Blood Oxygen Signal Based on CNN-Transformer Network.

In recent years, research on emotion recognition has become more and more popular, but there are few studies on emotion recognition based on cerebral blood oxygen signals. Since the electroencephalogram (EEG) is easily disturbed by eye movement and the portability is not high, this study uses a more comfortable and convenient functional near-infrared spectroscopy (fNIRS) system to record brain signals from participants while watching three different types of video clips. During the experiment, the changes in cerebral blood oxygen concentration in the 8 channels of the prefrontal cortex of the brain were collected and analyzed. We processed and divided the collected cerebral blood oxygen data, and used multiple classifiers to realize the identification of the three emotional states of joy, neutrality, and sadness. Since the classification accuracy of the convolutional neural network (CNN) in this research is not significantly superior to that of the XGBoost algorithm, this paper proposes a CNN-Transformer network based on the characteristics of time series data to improve the classification accuracy of ternary emotions. The network first uses convolution operations to extract channel features from multi-channel time series, then the features and the output information of the fully connected layer are input to the Transformer netork structure, and its multi-head attention mechanism is used to focus on different channel domain information, which has better spatiality. The experimental results show that the CNN-Transformer network can achieve 86.7% classification accuracy for ternary emotions, which is about 5% higher than the accuracy of CNN, and this provides some help for other research in the field of emotion recognition based on time series data such as fNIRS.

Bacteriological Investigation and Drug Resistance Analysis of Chronic Refractory Wound Secretions.

BACKGROUND: Chronic refractory wounds were common and the treatments were complicated for burn and plastic surgeons. This study was to investigate the bacterial distribution characteristics and bacterial drug resistance of chronic refractory wound secretions. METHODS: The authors retrospectively analyzed 425 patients with chronic refractory wound infection. The results of bacterial culture of wound secretions and drug sensitivity test were retrospectively analyzed. Further, the location area of the wound was divided into 4 regions, and the difference of the bacterial culture results between different regions was analyzed. RESULTS: The wound secretions were cultured into 401 bacterial strains, including 206 gram-positive bacteria strains, accounting for 51.4%, with the highest detection rate of Staphylococcus aureus at 26.2% (105/401). There were 195 gram-negative bacteria strains, accounting for 48.6%, with the highest detection rate of Pseudomonas aeruginosa at 14.2% (57/401). There were 6 fungal strains. The proportion of gram-negative bacteria in the III region of the wound zone was significantly greater than that in the other 3 regions. CONCLUSIONS: The detection rate of gram-positive bacteria and gram-negative bacteria of chronic refractory wound secretions is not much different. However, in the area close to the perineum (III region), gram-negative bacteria is significantly higher, which has a certain reference value for the use of antibiotics in clinical practice. LEVEL OF EVIDENCE: Level 4.

Control of transverse mode in a He-Ne laser using an astigmatic resonator.

The realization of output of the controllable transverse mode in a laser resonator has always been the key problem in applications of lasers. At present, the theory of optical resonators for passive resonators is relatively mature, but the non-uniformity of gain media greatly affects the output of the laser transverse mode for astigmatic resonators in operation; especially for gas lasers, controllable high-order modes have not been studied. To realize the theory of an astigmatic passive resonator of a gas laser as a good approximation of an active resonator, this paper develops the theory of selecting the laser eigenmode through an astigmatic resonator, and verifies that the two-dimensional tilt of the cavity mirror can break the axial symmetry. Controllable output of the laser mode is realized in real time and conveniently for the first time, to the best of our knowledge. This scheme is not only common to all kinds of lasers, but also has important research significance for the high-order modes required for real-time and rapid regulation of gas lasers under operating conditions.

Focal shift of an axisymmetric Bessel-Gaussian beam under Airy mixing modulation.

In this paper, the focusing characteristics of Bessel-Gaussian beams are studied by means of vector diffraction theory. The vector field distribution of the axisymmetric Bessel-Gaussian beam of a cylindrical vector is derived by calculating and adding Airy mixing modulation to the Bessel-Gaussian beam. It is found that with a series of regular focusing change characteristics, the focusing presents strong stability of the optical chain structure, and the number of optical chain links can be adjusted. At the same time, it is pointed out that in the case of a tightly focused helically polarized beam, the polarization in the focal region is not uniform, but there was a similar horizontal shift in focus. Finally, the relevant practical application scenarios are briefly introduced. The correlation focus shift conversion can be widely used in electronic acceleration, optical sampling and operation, and biological imaging.

Linc-OIP5 loss regulates migration and invasion in MDA-MB-231 breast cancer cells by inhibiting YAP1/JAG1 signaling.

Breast cancer is the most prevalent cancer among women, and diagnosis and treatment represent a substantial challenge due to the lack of adequate molecular targets. It has been shown that long noncoding RNAs (lncRNAs) serve pivotal roles in regulating gene expression in tumors. The roles of long intervening noncoding RNA (Linc)-OIP5 has been demonstrated in different types of cancer; however, its function in breast cancer has not been determined. In the present study, expression of Linc-OIP5, YAP1 (Hippo signaling component) and JAG1 (Notch signaling component) in breast cancer cells with different degrees of malignancy were determined. To assess whether Linc-OIP5 regulated the malignant biological behaviors of MDA-MB-231 cells, its expression was knocked down using a specific small interfering RNA (siRNA), and cell proliferation was determined using a CCK-8 assay, apoptosis was evaluated using an Annexin V-FITC apoptosis detection kit, migration was assessed using a wound healing and transwell migration assays, and cell invasion examined using a transwell invasion assays. The effect of Linc-OIP5 knockdown on YAP1 and JAG1 expression was quantified using reverse transcription-quantitative PCR and immunoblotting. Cell proliferation, migration and invasion were reduced, while apoptosis was increased in MDA-MB-231 cells transfected with Linc-OIP5-specific siRNA. Mechanistic investigations showed that Linc-OIP5 knockdown downregulated YAP1 and JAG1 expression. The results of the present study suggest that Linc-OIP5 affects the malignant biological behaviors of MDA-MB-231 cells, at least partly through its effects on YAP1/JAG1 signaling. Whilst there are a number of mechanisms underlying the pathogenesis of breast cancer, the results of the present study highlight Linc-OIP5 as a potential therapeutic target in breast cancer.

Upregulated circular RNA circ_0016760 indicates unfavorable prognosis in NSCLC and promotes cell progression through miR-1287/GAGE1 axis.

Non-small cell lung cancer (NSCLC) is an aggressive malignancy with increasing worldwide incidence and is characterized by dismal prognosis due to its early recurrence and metastasis. Accumulating evidence documented that aberrantly expressed circular RNAs (circRNAs) are critically involved in tumorigenesis. In the current study, we focused on a novel circRNA, circ_0016760 in NSCLC. qRT-PCR was carried out to determine the expression level of circ_0016760 in tissue samples and cell lines. The clinical value of circ_0016760 was also investigated. The functions of circ_0016760 was explored by CCK-8, colony formation, flow cytometry, Transwell and animal experiments. Luciferase reporter assays were conducted to investigate the association between circ_0016760 and miR-1287 and miR-1287 and GAGE1. We found that circ_0016760 was enhanced in NSCLC tissues and cells and the upregulation of circ_0016760 is associated with advanced TNM stages, lymph node metastasis and adverse prognosis in NSCLC patients. Moreover, circ_0016760 could significantly promote cell growth and metastatic properties and inhibit cell apoptosis in NSCLC cells. In mechanism, circ_0016760 functioned as a sponge for miR-1287 and regulated the expression of GAGE1. Subsequently, functional assays illustrated that the oncogenic properties of circ_0016760 is partly attribute to the regulation of miR-1287/GAGE1 axis. In summary, circ_0016760/miR-1287/GAGE1 signaling might play vital roles in the tumorigenesis and progression of NSCLC.

Control of the multifocal properties of composite vector beams in tightly focusing systems.

The numbers of the focal spots and the dominant field (i.e., whether the axial field or the transverse fields play dominant role in the focusing field) have significant effects on various applications. In this paper, we have derived the universal imaging model of the composite vector beam (CVB) composed of two orthogonally linearly polarized beams with inhomogeneous polarization modulation, which is also suitable for various polarized beams, such as linearly, circularly, radially, azimuthally, and vortex polarized beams. Moreover, the sin&cos amplitude modulation with arbitrary orders and the pupil filters with cylindrical symmetry are also involved in this imaging model. On the basis of this imaging model, the regulars to control the focal numbers and the dominant field are drawn. For the various applications, some important conclusions and constructive advices are given.

Multifocus with small size, uniform intensity, and nearly circular symmetry.

We investigate in detail the focusing properties of the composite vector beam (CVB) composed of two orthogonally linearly polarized beams with inhomogeneous polarization modulation. By optimizing the modulation factor, a multifocus with excellent quality is obtained, where the sizes of each focus are fairly smaller than that of the focusing spot of a radially polarized beam, the uniformity in the intensity of the focal spots is as high as 1, and the distributions of each focal spot have nearly circular symmetry. In order to decrease the power loss of the incident beam, the CVB formed by an annular beam is demonstrated as the substitute for the optimized CVB formed by a Gaussian beam. This work is important for high-resolution and high-speed imaging in biology and micro-nanofabrication.