Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 8 de 8
Filter
Add more filters










Database
Language
Publication year range
1.
Molecules ; 25(3)2020 Jan 29.
Article in English | MEDLINE | ID: mdl-32013151

ABSTRACT

To clarify whether flavonoids and phenols in Allium mongolicum Regel have the effect of improving gastrointestinal function and analyze its quality, this study was designed to isolate and identify them from the aerial parts of A. mongolicum by using various chromatographic and spectrophotometric methods, a bioassay on motility of mouse isolated intestine tissue, as well as qualitative analysis using liquid chromatography/mass spectrometry (LC-MS) analysis. As a result, 31 flavonoids and phenolic acids were obtained and identified, including six new flavonoid glycosides, mongoflavonosides A1 (1), A2 (2), A3 (3), A4 (4), B1 (5), B2 (6), and four new phenolic acid glycosides, mongophenosides A1 (7), A2 (8), A3 (9), B (10). Among them, eleven flavonoids and three phenolic acids showed significant increase in the height of mouse small intestinal muscle. It was a first systematic bioactive constituents' study for A. mongolicum on gastrointestinal tract. Furthermore, according to the retention time (tR) and the exact mass-to-charge ratio (m/z), thirty-one compounds were unambiguously identified by comparing to the standard references by using LC-MS. Then, on the basis of generalized rules of MS/MS fragmentation pattern, chromatographic behaviors, as well as biosynthetic laws of the 31 isolates, five flavonoid glycosides and one phenolic acid glycoside were tentatively speculated. On the basis of the study, a fast analysis method for flavonoids and phenolic acids in A. mongolicum was established.


Subject(s)
Allium/chemistry , Flavonoids/chemistry , Hydroxybenzoates/chemistry , Phytochemicals/chemistry , Plant Components, Aerial/chemistry , Plant Extracts/chemistry , Animals , Flavonoids/pharmacology , Gastrointestinal Motility/drug effects , Hydroxybenzoates/pharmacology , Mice , Molecular Structure , Phytochemicals/pharmacology , Plant Extracts/pharmacology , Spectrum Analysis
2.
Steroids ; 112: 95-102, 2016 08.
Article in English | MEDLINE | ID: mdl-27234504

ABSTRACT

Seven new steroid glycosides, dioscorosides A1 (1), A2 (2), B1 (3), B2 (4), C1 (5), C2 (6), and D (7), together with 22 known ones (8-29) were isolated from the rhizomes of Dioscorea septemloba, their structures were elucidated by chemical and spectroscopic methods. All isolates were evaluated for in vitro anti-inflammatory potential using LPS-stimulated RAW 264.7 murine macrophages. Among them, spirostane glycosides 18 and 21-24 exhibited significant inhibition of nitrite production. Moreover, the structure-activity relationship was summarized.


Subject(s)
Dioscorea/chemistry , Rhizome/chemistry , Steroids/chemistry , Animals , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/pharmacology , Macrophages/drug effects , Magnetic Resonance Spectroscopy , Mice , Molecular Structure , Nitrogen Oxides/metabolism , RAW 264.7 Cells , Steroids/pharmacology , Structure-Activity Relationship
3.
Molecules ; 21(3): 354, 2016 Mar 16.
Article in English | MEDLINE | ID: mdl-26999087

ABSTRACT

Four new aromatic constituents, astraflavonoids A (1), B (2), C (3), and astramemoside A (4), along with sixteen known ones 5-20 were obtained from the stems of A. membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao. Their structures were elucidated by chemical and spectroscopic methods. Among the known isolates, 14 was obtained from the Astragalus genus for the first time, while 7-12, 18-20 were isolated from the species for the first time. The effects of the compounds obtained from the plant on glucose consumption were analyzed in differentiated L6 myotubes in vitro, whereby compounds 1, 2, 3, 7, 8, 10, 11, 14, 15 and 18 displayed significant promoting effects on glucose consumption in L6 myotubes. Among them, the activities of 1, 2 and 7 were comparable to that of insulin, which suggested that these compounds may be involved in glucose metabolism and transport.


Subject(s)
Astragalus propinquus/chemistry , Organic Chemicals/chemistry , Plant Stems/chemistry , Astragalus propinquus/metabolism , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Stems/metabolism , Structure-Activity Relationship
4.
Fitoterapia ; 109: 99-105, 2016 Mar.
Article in English | MEDLINE | ID: mdl-26687558

ABSTRACT

Astragalus membranaceus is a Traditional Chinese Medicine (TCM) belonging to the Leguminosae family. It has been used as antidiabetic, cardioprotective, and hepatoprotective in the TCM clinic. From the stems of A. membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao, 14 oleanane type saponins (1-14) including eight new ones, astroolesaponins A (1), B (2), C1 (3), C2 (4), D (5), E1 (6), E2 (7), and F (8) were obtained, and their structures were elucidated by chemical and spectroscopic methods. Compounds 1-5, 7, 8, 11, and 13 showed decreased effects on triglyceride levels in sodium oleate induced HepG2 cells.


Subject(s)
Astragalus propinquus/chemistry , Oleanolic Acid/analogs & derivatives , Saponins/chemistry , Hep G2 Cells , Humans , Molecular Structure , Oleanolic Acid/chemistry , Oleanolic Acid/isolation & purification , Plants, Medicinal/chemistry , Saponins/isolation & purification
5.
Molecules ; 20(12): 21946-59, 2015 Dec 08.
Article in English | MEDLINE | ID: mdl-26670227

ABSTRACT

Five new compounds, lippianosides A (1), B (2), C (3), D (4), and E (5), along with 26 (6-31) known ones were obtained from the 95% EtOH extract of Lippia triphylla (L. triphylla) aerial parts collected from Rwanda, Africa. Among the known compounds, 11 and 17-30 were isolated from the Lippia genus for the first time. In addition, 12, 13, and 16 were firstly obtained from this species. The structures of them were elucidated by chemical and spectroscopic methods. The antioxidant and triglyceride accumulation inhibition effects of the 31 compounds were examined in L6 cells and HepG2 cells, respectively.


Subject(s)
Antineoplastic Agents/pharmacology , Antioxidants/pharmacology , Cell Proliferation/drug effects , Lippia/chemistry , Muscle, Skeletal/drug effects , Plant Components, Aerial/chemistry , Triterpenes/pharmacology , Animals , Antineoplastic Agents/chemistry , Antioxidants/chemistry , Cells, Cultured , Hep G2 Cells , Humans , Muscle, Skeletal/pathology , Rats , Triglycerides/metabolism , Triterpenes/chemistry
6.
Molecules ; 20(8): 13982-96, 2015 Aug 03.
Article in English | MEDLINE | ID: mdl-26247923

ABSTRACT

Seven new rare sinapoyl acylated flavonoid glycosides, apetalumosides A1 (1), B8 (2), B9 (3), B10 (4), B11 (5), B12 (6), and C1 (7) were isolated from the seeds of Lepidium apetalum Willd. Their structures were elucidated by chemical and spectroscopic methods.


Subject(s)
Acyl Coenzyme A/chemistry , Flavonoids/isolation & purification , Glycosides/isolation & purification , Lepidium/chemistry , Seeds/chemistry , Acylation , Carbon-13 Magnetic Resonance Spectroscopy , Dimethyl Sulfoxide/chemistry , Flavonoids/chemistry , Glycosides/chemistry , Proton Magnetic Resonance Spectroscopy
7.
Fitoterapia ; 102: 7-14, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25677352

ABSTRACT

Hollyhock (Althaea rosea (Linn.) Cavan) belongs to Althaea genus, Malvaceae family, is a perennial garden plant distributed throughout the world in warm temperate and tropical regions, which was used as anti-diabetes ingredient in traditional Chinese medicine. In the process of our research, ethanolic extract of hollyhock flower (HFE) was found to decrease serum triglyceride and glucose levels significantly in KK-A(y) mice after oral administration for 8weeks. Meanwhile, gene expressions on AMPK, IRS2, PI3K, AKT and GLUT4 in liver were remarkably up-regulated. Three new dihydroflavonol glycosides, named as roseaflavanonolosides A (1), B (2), and C (3), together with two known ones were obtained from HFE. Their structures were elucidated by chemical and spectroscopic methods. Hepatic cell glucose uptake experiment was performed using 2-NBDG as a glucose uptake indicator. At the dosage of 20µg/mL for 1-5, the glucose uptake increasing level was nearly 30%-40% in HepG2 cells. We partly revealed the glucose metabolism regulation effect may relate to dihydroflavonols in hollyhock flower.


Subject(s)
Althaea/chemistry , Flavonols/pharmacology , Flowers/chemistry , Glycosides/pharmacology , Hypoglycemic Agents/pharmacology , Animals , Blood Glucose/drug effects , Flavonols/isolation & purification , Glycosides/isolation & purification , Hep G2 Cells , Humans , Liver/drug effects , Liver/metabolism , Male , Mice, Inbred C57BL , Molecular Structure , Triglycerides/blood
8.
Molecules ; 19(11): 18850-80, 2014 Nov 17.
Article in English | MEDLINE | ID: mdl-25407722

ABSTRACT

Astragalus L., is one of the largest genuses of flowering plants in the Leguminosae family. Roots of A. membranaceus Bge. var. mongholicus (Bge.) Hsiao, A. membranaceus (Fisch.) Bge. and its processed products are listed in the China Pharmacopeia for "qi deficiency" syndrome treatment. However, more and more researches on other species of Astragalus have been conducted recently. We summarize the recent researches of Astragalus species in phytochemistry and pharmacology. More than 200 constituents, including saponins and flavonoids, obtained from 46 species of Astragalus genus were collected for this article. In pharmacological studies, crude extracts of Astragalus, as well as isolated constituents showed anti-inflammatory, immunostimulant, antioxidative, anti-cancer, antidiabetic, cardioprotective, hepatoprotective, and antiviral activities. The goal of this article is to provide an overview of chemical and pharmacological studies on the Astragalus species over the last 10 years, which could be of value to new drug or food supplement research and development.


Subject(s)
Astragalus Plant/chemistry , Astragalus Plant/metabolism , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plants, Medicinal/chemistry , Animals , Dietary Supplements , Flavonoids/chemistry , Flavonoids/pharmacology , Saponins/chemistry , Saponins/pharmacology
SELECTION OF CITATIONS
SEARCH DETAIL
...