Facile green synthesis of silver nanoparticles using Mangifera indica seed aqueous extract and its antimicrobial, antioxidant and cytotoxic potential (3-in-1 system).

A novel approach for the utilisation of fruit waste is attempted in the present investigation. Mangifera indica seed aqueous extract was utilised for green synthesis of silver nanoparticles (AgNPs). The phytoconstituents in the seed acted as reducing and stabilising agent for AgNP formation. UV-Vis, Zeta potential, FT-IR, XRD, TEM, SAED, EDX analysis were used to characterise the green synthesised AgNPs. UV-vis spectra showed characteristic spectra at 450 nm; XRD and SAED confirmed the crystalline nature while TEM revealed the shape to be round and average size was 26.85 nm. FT-IR revealed functional groups like alcohol or phenols, carboxylic acids, ketones, amines, aromatic amines, aliphatic amines, alkyl halides and alkynes which were responsible for AgNP formation. The nanoparticles showed more antibacterial activity than antifungal activity and antibacterial activity towards Gram-negative bacteria was more than Gram-positive bacteria. Dose dependent antioxidant activity (DPPH, SO and ABTS) and dose dependent cytotoxic effect against HeLa, MCF-7 and normal fibroblast cell lines was envisaged. The green synthesised AgNPs exhibited three different bioactivities (3-in-1 system) i.e. dose dependent antimicrobial, antioxidant and cytotoxic activity. Fruit waste can be successfully utilised for silver nanoparticles formation which can be therapeutically useful and effective.[Figure: see text]HighlightsSilver nanoparticles were synthesised from M. indica fruit waste i.e. seedCharacterisation by spectroscopic techniques: UV-Vis, Zeta, FTIR, XRD, SAED, EDX and TEM analysis.Silver nanoparticles were 26.85 nm in size and round in shapeAntimicrobial activity against 14 microorganismsAntioxidant activity in terms of DPPH, SO and ABTSCytotoxic activity against HeLa, MCF-7 and Fibroblast normal cell lines.

Antimicrobial, antioxidant and anticancer activities of gold nanoparticles green synthesized using Mangifera indica seed aqueous extract.

In this study, gold nanoparticles (AuNPs) were synthesised using seed extract of mango (Mangifera indica) which is considered as waste and generally thrown away into the environment. The bioactive molecules in the seed act as reducing agent to synthesise AuNPs without using any external agent. The characterisation of green synthesised AuNPs was done using various spectroscopic techniques. Visual colour change from colourless to ruby red colour confirmed the formation of AuNPs which was further confirmed by maximum absorption peak at 550 nm by UV-spectra. Crystalline nature was confirmed by XRD technique while round, triangle and irregular shape and 19.45 nm size was confirmed by TEM and SAED analysis. FTIR analysis confirmed the presence of alcohol or phenol, carboxylic acid, ketones, amines, aromatic amines, aliphatic amines, alkyl halides and alkynes in M. indica seed which were responsible for the reduction of gold to AuNPs. The green synthesised AuNPs were evaluated for their antimicrobial, antioxidant and cytotoxic potential. They showed moderate antibacterial, cytotoxic and dose-dependent antioxidant activity. Seeds of M. indica instead of discarding can be successfully utilised for AuNPs synthesis which can be used as a natural source of antimicrobial, antioxidant and anticancer agent. Highlights Green synthesis of gold nanoparticles (AuNPs) from fruit (Mangifera indica) waste (seed). Characterisation using various spectroscopic techniques: UV-Vis spectroscopy, Zeta potential, FTIR, XRD and TEM analysis. Synthesized AuNPs were round, triangle and irregular in shape and 19.45 nm in size. Antimicrobial activity of AuNPs against 14 microorganisms. Antioxidant activity of AuNPs in terms of DPPH, SO and ABTS. Cytotoxic activity against HeLa, MCF-7 and fibroblast normal cell lines.